Construct: ORF TRCN0000472118
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007774.1_s317c1
- Derived from:
- ccsbBroadEn_02052
- DNA Barcode:
- GGGCGCATCCCAGTACGAATTCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KYNU (8942)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472118
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8942 | KYNU | kynureninase | NM_001032998.2 | 100% | 100% | |
| 2 | human | 8942 | KYNU | kynureninase | XM_017005217.1 | 100% | 100% | |
| 3 | human | 8942 | KYNU | kynureninase | NM_001199241.2 | 65.6% | 64.9% | (many diffs) |
| 4 | human | 8942 | KYNU | kynureninase | NM_003937.3 | 65.6% | 64.9% | (many diffs) |
| 5 | human | 8942 | KYNU | kynureninase | XM_024453208.1 | 65.6% | 64.9% | (many diffs) |
| 6 | human | 8942 | KYNU | kynureninase | XR_923058.1 | 51.8% | (many diffs) | |
| 7 | human | 8942 | KYNU | kynureninase | XM_011512102.2 | 37% | 36.3% | (many diffs) |
| 8 | mouse | 70789 | Kynu | kynureninase (L-kynurenine ... | NM_001289594.1 | 80.9% | 79.6% | (many diffs) |
| 9 | mouse | 70789 | Kynu | kynureninase (L-kynurenine ... | NM_001289593.1 | 61.7% | 60.5% | (many diffs) |
| 10 | mouse | 70789 | Kynu | kynureninase (L-kynurenine ... | NM_027552.2 | 56.8% | 55.6% | (many diffs) |
| 11 | mouse | 70789 | Kynu | kynureninase (L-kynurenine ... | XM_006498325.3 | 56.8% | 55.6% | (many diffs) |
| 12 | mouse | 70789 | Kynu | kynureninase (L-kynurenine ... | XM_011239178.1 | 52.2% | 50.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 987
- ORF length:
- 921
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gccttcatct cttgagctgc cggctgacac agtgcagcgc attgcggctg 121 aactcaaatg ccacccaacg gatgagaggg tggctctcca cctagatgag gaagataagc 181 tgaggcactt cagggagtgc ttttatattc ccaaaataca ggatctgcct ccagttgatt 241 tatcattagt gaataaagat gaaaatgcca tctatttctt gggaaattct cttggccttc 301 aaccaaaaat ggttaaaaca tatcttgaag aagaactaga taagtgggcc aaaatagcag 361 cctatggtca tgaagtgggg aagcgtcctt ggattacagg agatgagagt attgtaggcc 421 ttatgaagga cattgtagga gccaatgaga aagaaatagc cctaatgaat gctttgactg 481 taaatttaca tcttctaatg ttatcatttt ttaagcctac gccaaaacga tataaaattc 541 ttctagaagc caaagccttc ccttcTGATC ATTATGCTAT TGAGTCACAA CTACAACTTC 601 ACGGACTTAA CATTGAAGAA AGTATGCGGA TGATAAAGCC AAGAGAGGGG GAAGAAACCT 661 TAAGAATAGA GGATATCCTT GAAGTAATTG AGAAGGAAGG AGACTCAATT GCAGTGATCC 721 TGTTCAGTGG GGTGCATTTT TACACTGGAC AGCACTTTAA TATTCCTGCC ATCACAAAAG 781 CTGGACAAGC GAAGGGTTGT TATGTTGGCT TTGATCTAGC ACATGCAGTT GGAAATGTTG 841 AACTCTACTT ACATGACTGG GGAGTTGATT TTGCCTGCTG GTGTTCCTAC AAGTATTTAA 901 ATGCAGGAGC AGGAGGAATT GCTGGTGCCT TCATTCATGA AAAGCATGCC CATACGATTA 961 AACCTGCGAG ATCGGAGTTC TTTAATTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1021 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1081 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGGCGCAT 1141 CCCAGTACGA ATTCCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1201 aagatt