Transcript: Human XR_924162.3

PREDICTED: Homo sapiens major facilitator superfamily domain containing 1 (MFSD1), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MFSD1 (64747)
Length:
2865
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_924162.3
NBCI Gene record:
MFSD1 (64747)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_924162.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000059688 GCAAGTGAATACCACGAAATT pLKO.1 1111 3UTR 100% 13.200 18.480 N MFSD1 n/a
2 TRCN0000333604 GCAAGTGAATACCACGAAATT pLKO_005 1111 3UTR 100% 13.200 18.480 N MFSD1 n/a
3 TRCN0000344891 GGGCATAATGCTAGGGAATAT pLKO_005 2587 3UTR 100% 13.200 18.480 N MFSD1 n/a
4 TRCN0000344948 TATCTGTGGTCTTACTCTATT pLKO_005 2030 3UTR 100% 13.200 18.480 N MFSD1 n/a
5 TRCN0000059689 CCTCGGGATCACACTTATGAT pLKO.1 1519 3UTR 100% 5.625 7.875 N MFSD1 n/a
6 TRCN0000059692 GTCTTACTCTATTTGGTGAAT pLKO.1 2038 3UTR 100% 4.950 6.930 N MFSD1 n/a
7 TRCN0000344892 ATCTTGGGTTGGCCATCATTT pLKO_005 1925 3UTR 100% 13.200 9.240 N MFSD1 n/a
8 TRCN0000059690 CCTTAGCAGTTGCCCAGAATA pLKO.1 1344 3UTR 100% 13.200 9.240 N MFSD1 n/a
9 TRCN0000344890 GCCCTTCAGACTCAAGTTAAA pLKO_005 1082 3UTR 100% 13.200 9.240 N MFSD1 n/a
10 TRCN0000059691 GTGGCATTTGTAGTTCCTGAA pLKO.1 1861 3UTR 100% 4.050 2.835 N MFSD1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_924162.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14246 pDONR223 100% 40.8% None (many diffs) n/a
2 ccsbBroad304_14246 pLX_304 0% 40.8% V5 (not translated due to frame shift) (many diffs) n/a
3 TRCN0000472389 CAAACTAGACCTTGCACTCTCTGA pLX_317 30.9% 40.8% V5 (not translated due to frame shift) (many diffs) n/a
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