Construct: ORF TRCN0000472389
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004232.1_s317c1
- Derived from:
- ccsbBroadEn_14246
- DNA Barcode:
- CAAACTAGACCTTGCACTCTCTGA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MFSD1 (64747)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472389
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64747 | MFSD1 | major facilitator superfami... | NM_022736.4 | 99.7% | 99.5% | 502A>G;1393G>A;1395delA |
| 2 | human | 64747 | MFSD1 | major facilitator superfami... | NM_001167903.2 | 91.3% | 87.9% | (many diffs) |
| 3 | human | 64747 | MFSD1 | major facilitator superfami... | NM_001289407.2 | 84% | 83.8% | (many diffs) |
| 4 | human | 64747 | MFSD1 | major facilitator superfami... | XM_006713730.2 | 82.6% | 79.5% | (many diffs) |
| 5 | human | 64747 | MFSD1 | major facilitator superfami... | NM_001289406.2 | 78.9% | 75.8% | (many diffs) |
| 6 | human | 64747 | MFSD1 | major facilitator superfami... | NR_110328.2 | 54.2% | (many diffs) | |
| 7 | human | 64747 | MFSD1 | major facilitator superfami... | XR_924162.3 | 40.8% | (many diffs) | |
| 8 | mouse | 66868 | Mfsd1 | major facilitator superfami... | NM_025813.3 | 85% | 87.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1461
- ORF length:
- 1395
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggaggaggat gaggaagcgc gggcgctcct ggcaggcggc cctgacgagg 121 ccgacagagg tgccccggcc gcccctggag ccctgccggc cctctgcgac cccagtcgcc 181 tggcgcaccg gcttttggtg ctgttactga tgtgcttcct tggctttggc agctattttt 241 gctatgataa tcctgctgcc cttcagactc aagttaaacg agatatgcaa gtgaatacca 301 cgaaattcat gctgctgtat gcctggtatt cttggcccaa tgtagttttg tgtttctttg 361 gtggcttttt gatagaccga gtatttggaa tacgatgggg cacaatcatt tttagctgct 421 ttgtttgcat tggacaggtt gtttttgccc tgggtggaat atttaatgct ttttggctga 481 tggaatttgg aagatttgta tttgggattg gtggcgagtc cttagcagtt gcccagaata 541 catatgctgt gagctggttt aaaggcgaag aattaaacct ggtgtttgga cttcaactta 601 gcatggctag aattggaagt acagtaaaca tgaacctcat gggatggctg tattctaaga 661 ttgaagcttt gttaggttct gctggtcaca caaccctcgg gatcacactt atgattgggg 721 gtataacgtg tattctttca ctaatctgtg ccttggctct tgcctacttg gatcagagag 781 cagagagaat ccttcataaa gaacaaggaa aaacaggtga agttattaaa ttaactgatg 841 taaaggactt ctccttaccc ctgtggctta tatttatcat ctgtgtctgc tattatgttg 901 ctgtgttccc ttttattgga cttgggaaag ttttctttac agagaaattt ggattttctt 961 cccaggcagc aagtgcaatt aacagtgttg tatatgtcat atcagctccc atgtccccgg 1021 tgtttgggct ccTGGTGGAT AAAACAGGGA AGAACATCAT CTGGGTTCTT TGCGCAGTAG 1081 CAGCCACTCT TGTGTCCCAC ATGATGCTGG CCTTTACGAT GTGGAACCCT TGGATTGCTA 1141 TGTGTCTTCT GGGACTCTCC TACTCATTGC TTGCCTGTGC ATTGTGGCCA ATGGTGGCAT 1201 TTGTAGTTCC TGAACATCAG CTGGGAACTG CATATGGCTT CATGCAGTCC ATTCAGAATC 1261 TTGGGTTGGC CATCATTTCC ATCATTGCTG GTATGATACT GGATTCTCGG GGGTATTTGT 1321 TTTTGGAAGT GTTCTTCATT GCCTGTGTTT CTTTGTCACT TTTATCTGTG GTCTTACTCT 1381 ATTTGGTGAA TCGTGCCCAG GGTGGGAACC TAAATTATTC TGCAAGACAA AGGGAAGAAA 1441 TAAAATTTTC CCATACTAAT GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC 1501 TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA 1561 AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACAAAC TAGACCTTGC 1621 ACTCTCTGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt