Transcript: Human XR_927527.2

PREDICTED: Homo sapiens syntaxin 1A (STX1A), transcript variant X2, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
STX1A (6804)
Length:
2312
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_927527.2
NBCI Gene record:
STX1A (6804)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_927527.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000218716 AGAACTCATGTCCGACATAAA pLKO_005 270 3UTR 100% 13.200 18.480 N STX1A n/a
2 TRCN0000230306 CAAGCCAGCGTTGCATGTTTG pLKO_005 1852 3UTR 100% 10.800 15.120 N STX1A n/a
3 TRCN0000257113 CAAAGTTCGTTCCAAGTTAAA pLKO_005 303 3UTR 100% 13.200 9.240 N STX1A n/a
4 TRCN0000257133 AGGAGATTCGAGGCTTCATTG pLKO_005 155 3UTR 100% 10.800 7.560 N STX1A n/a
5 TRCN0000230305 TTGACAGGATCGAGTACAATG pLKO_005 937 3UTR 100% 10.800 7.560 N STX1A n/a
6 TRCN0000236766 TTGACAGGATCGAGTACAATG pLKO_005 937 3UTR 100% 10.800 7.560 N Stx1a n/a
7 TRCN0000065012 CATAAAGAAGACAGCAAACAA pLKO.1 285 3UTR 100% 5.625 3.938 N STX1A n/a
8 TRCN0000065010 GAGGAGATTCGAGGCTTCATT pLKO.1 154 3UTR 100% 5.625 3.938 N STX1A n/a
9 TRCN0000382305 ACTCCACGCTGTCCAGAAAGT pLKO_005 401 3UTR 100% 4.950 3.465 N STX1A n/a
10 TRCN0000381347 CTCTGTGAGTGTGCGTCTGTA pLKO_005 1296 3UTR 100% 4.950 3.465 N STX1A n/a
11 TRCN0000381955 CTTTGCCTCTGGGATCATCAT pLKO_005 570 3UTR 100% 4.950 3.465 N STX1A n/a
12 TRCN0000380398 GAACACGCGGTAGACTATGTG pLKO_005 960 3UTR 100% 4.950 3.465 N STX1A n/a
13 TRCN0000065008 GAAGAACTCATGTCCGACATA pLKO.1 268 3UTR 100% 4.950 3.465 N STX1A n/a
14 TRCN0000381844 GGAGGTCATGTCGGAGTACAA pLKO_005 426 3UTR 100% 4.950 3.465 N STX1A n/a
15 TRCN0000110583 GATGATTGACAGGATCGAGTA pLKO.1 932 3UTR 100% 4.050 2.835 N Stx1a n/a
16 TRCN0000380705 TGAGTCTCAGTCGCTGATCAC pLKO_005 1606 3UTR 100% 4.050 2.835 N STX1A n/a
17 TRCN0000065011 AGGATCGAGTACAATGTGGAA pLKO.1 942 3UTR 100% 2.640 1.848 N STX1A n/a
18 TRCN0000065009 CCAGAAAGTTTGTGGAGGTCA pLKO.1 413 3UTR 100% 2.640 1.848 N STX1A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_927527.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07013 pDONR223 100% 37.3% None (many diffs) n/a
2 ccsbBroad304_07013 pLX_304 0% 37.3% V5 (many diffs) n/a
3 TRCN0000481524 AGCCGTACAACCACTACAGACGTG pLX_317 60.1% 37.3% V5 (many diffs) n/a
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