Construct: ORF TRCN0000481524
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011106.2_s317c1
- Derived from:
- ccsbBroadEn_07013
- DNA Barcode:
- AGCCGTACAACCACTACAGACGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STX1A (6804)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481524
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6804 | STX1A | syntaxin 1A | NM_004603.4 | 99.8% | 99.6% | 217G>A |
| 2 | human | 6804 | STX1A | syntaxin 1A | NM_001165903.2 | 87% | 79.4% | 217G>A;676_677ins46;753_754ins65 |
| 3 | human | 6804 | STX1A | syntaxin 1A | XM_017012567.2 | 77.7% | 69.5% | (many diffs) |
| 4 | human | 6804 | STX1A | syntaxin 1A | XR_242263.2 | 39.2% | (many diffs) | |
| 5 | human | 6804 | STX1A | syntaxin 1A | XR_927527.2 | 37.3% | (many diffs) | |
| 6 | mouse | 20907 | Stx1a | syntaxin 1A (brain) | NM_016801.4 | 91% | 97.9% | (many diffs) |
| 7 | mouse | 20907 | Stx1a | syntaxin 1A (brain) | XM_006504400.3 | 78% | 70.5% | (many diffs) |
| 8 | mouse | 20907 | Stx1a | syntaxin 1A (brain) | NR_133683.1 | 35.1% | (many diffs) | |
| 9 | mouse | 20907 | Stx1a | syntaxin 1A (brain) | XR_377104.1 | 33.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 930
- ORF length:
- 864
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggaccgaacc caggagctcc gcacggccaa ggacagcgat gatgatgatg 121 atgtcgctgt caccgtggac cgagaccgct tcatggatga gttctttgag caggtggagg 181 agattcgagg cttcattgac aagatcgcag agaacgtgga ggaggtgaag cggaagcaca 241 gtgccatcct ggcatccccc aaccccgatg agaagacgaa gaaggagctg gaagaactca 301 tgtccgacat aaagaagaca gcaaacaaag ttcgttccaa gttaaagagc atcgagcagt 361 ccatcgagca agaggaaggc ctgaaccgct cctccgctga cctgaggaTC CGGAAGACAC 421 AGCACTCCAC GCTGTCCAGA AAGTTTGTGG AGGTCATGTC GGAGTACAAC GCCACGCAGT 481 CCGACTACCG CGAGCGCTGC AAAGGCCGCA TCCAGAGGCA GCTGGAGATC ACCGGCAGGA 541 CCACGACCAG TGAGGAGCTG GAGGACATGC TGGAGAGTGG GAACCCCGCC ATCTTTGCCT 601 CTGGGATCAT CATGGACTCC AGCATCTCGA AGCAGGCTCT GAGCGAGATT GAGACGCGGC 661 ACAGTGAGAT CATCAAGCTG GAGAACAGCA TCCGTGAGCT ACACGACATG TTCATGGACA 721 TGGCCATGCT CGTGGAGAGC CAGGGAGAGA TGATTGACAG GATCGAGTAC AATGTGGAAC 781 ACGCGGTAGA CTATGTGGAG AGGGCCGTGT CTGACACCAA GAAGGCCGTC AAGTACCAGA 841 GCAAGGCGCG CCGGAAGAAA ATCATGATCA TCATCTGCTG TGTGATCCTG GGCATCGTCA 901 TCGCCTCCAC TGTTGGGGGC ATCTTCGCCT ACCTAACTTT CTTGTACAAA GTGGTTGATA 961 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1021 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGCCG 1081 TACAACCACT ACAGACGTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1141 tgaaagatt