Construct: ORF TRCN0000481524

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011106.2_s317c1
Derived from:
ccsbBroadEn_07013
DNA Barcode:
AGCCGTACAACCACTACAGACGTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STX1A (6804)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481524
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6804 STX1A syntaxin 1A NM_004603.4 99.8% 99.6% 217G>A
2 human 6804 STX1A syntaxin 1A NM_001165903.2 87% 79.4% 217G>A;676_677ins46;753_754ins65
3 human 6804 STX1A syntaxin 1A XM_017012567.2 77.7% 69.5% (many diffs)
4 human 6804 STX1A syntaxin 1A XR_242263.2 39.2% (many diffs)
5 human 6804 STX1A syntaxin 1A XR_927527.2 37.3% (many diffs)
6 mouse 20907 Stx1a syntaxin 1A (brain) NM_016801.4 91% 97.9% (many diffs)
7 mouse 20907 Stx1a syntaxin 1A (brain) XM_006504400.3 78% 70.5% (many diffs)
8 mouse 20907 Stx1a syntaxin 1A (brain) NR_133683.1 35.1% (many diffs)
9 mouse 20907 Stx1a syntaxin 1A (brain) XR_377104.1 33.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
930
ORF length:
864
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa ggaccgaacc caggagctcc gcacggccaa ggacagcgat gatgatgatg
121atgtcgctgt caccgtggac cgagaccgct tcatggatga gttctttgag caggtggagg
181agattcgagg cttcattgac aagatcgcag agaacgtgga ggaggtgaag cggaagcaca
241gtgccatcct ggcatccccc aaccccgatg agaagacgaa gaaggagctg gaagaactca
301tgtccgacat aaagaagaca gcaaacaaag ttcgttccaa gttaaagagc atcgagcagt
361ccatcgagca agaggaaggc ctgaaccgct cctccgctga cctgaggaTC CGGAAGACAC
421AGCACTCCAC GCTGTCCAGA AAGTTTGTGG AGGTCATGTC GGAGTACAAC GCCACGCAGT
481CCGACTACCG CGAGCGCTGC AAAGGCCGCA TCCAGAGGCA GCTGGAGATC ACCGGCAGGA
541CCACGACCAG TGAGGAGCTG GAGGACATGC TGGAGAGTGG GAACCCCGCC ATCTTTGCCT
601CTGGGATCAT CATGGACTCC AGCATCTCGA AGCAGGCTCT GAGCGAGATT GAGACGCGGC
661ACAGTGAGAT CATCAAGCTG GAGAACAGCA TCCGTGAGCT ACACGACATG TTCATGGACA
721TGGCCATGCT CGTGGAGAGC CAGGGAGAGA TGATTGACAG GATCGAGTAC AATGTGGAAC
781ACGCGGTAGA CTATGTGGAG AGGGCCGTGT CTGACACCAA GAAGGCCGTC AAGTACCAGA
841GCAAGGCGCG CCGGAAGAAA ATCATGATCA TCATCTGCTG TGTGATCCTG GGCATCGTCA
901TCGCCTCCAC TGTTGGGGGC ATCTTCGCCT ACCTAACTTT CTTGTACAAA GTGGTTGATA
961TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1021CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGCCG
1081TACAACCACT ACAGACGTGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1141tgaaagatt

Download FASTA (ORF) (Full)