Transcript: Human XR_935231.2

PREDICTED: Homo sapiens zinc finger protein 532 (ZNF532), transcript variant X19, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZNF532 (55205)
Length:
2846
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_935231.2
NBCI Gene record:
ZNF532 (55205)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_935231.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000218616 TGCAATGCTCCCACTTAATTT pLKO_005 2289 3UTR 100% 15.000 21.000 N ZNF532 n/a
2 TRCN0000230136 CGGTGAAAGCCACGGTCATAT pLKO_005 1689 3UTR 100% 13.200 18.480 N ZNF532 n/a
3 TRCN0000242174 CGGTGAAAGCCACGGTCATAT pLKO_005 1689 3UTR 100% 13.200 18.480 N Zfp532 n/a
4 TRCN0000153605 CCAGATATGGTCGATCCTAAA pLKO.1 326 3UTR 100% 10.800 15.120 N ZNF532 n/a
5 TRCN0000154157 CGTCAAGAATGTTCGGAACAT pLKO.1 457 3UTR 100% 4.950 6.930 N ZNF532 n/a
6 TRCN0000156384 CGTTGCGCCATCAAAGACAAA pLKO.1 1030 3UTR 100% 4.950 6.930 N ZNF532 n/a
7 TRCN0000151697 CCAAACCTCAGCAACAAATAA pLKO.1 1899 3UTR 100% 15.000 10.500 N ZNF532 n/a
8 TRCN0000156932 GCAAAGAACCAGTGGCCAATT pLKO.1 1125 3UTR 100% 10.800 7.560 N ZNF532 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_935231.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14189 pDONR223 100% 67.2% None 1_265del;2612_2721del;2846_2847ins829 n/a
2 ccsbBroad304_14189 pLX_304 0% 67.2% V5 (not translated due to prior stop codon) 1_265del;2612_2721del;2846_2847ins829 n/a
3 TRCN0000476591 TGGGGGAACTCGCTGTCTTTAGGC pLX_317 9.1% 67.2% V5 (not translated due to prior stop codon) 1_265del;2612_2721del;2846_2847ins829 n/a
4 TRCN0000479609 CGTCGCCAGGTAAAATCCAATCGC pLX_317 8.6% 67.2% V5 (not translated due to prior stop codon) 1_265del;2612_2721del;2846_2847ins829 n/a
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