Transcript: Human XR_938772.2

PREDICTED: Homo sapiens DnaJ heat shock protein family (Hsp40) member B14 (DNAJB14), transcript variant X3, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
DNAJB14 (79982)
Length:
1079
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_938772.2
NBCI Gene record:
DNAJB14 (79982)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_938772.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000335861 GCGATCAAAGCAAGCCTAATT pLKO_005 351 3UTR 100% 13.200 18.480 N DNAJB14 n/a
2 TRCN0000154348 CGGGCAATGAAGAACAAGCAT pLKO.1 654 3UTR 100% 3.000 2.400 N DNAJB14 n/a
3 TRCN0000335863 ATCCAGCTGATGCCCATAATT pLKO_005 1009 3UTR 100% 15.000 10.500 N DNAJB14 n/a
4 TRCN0000150653 GAGGTTGTGAAGCTGATATAA pLKO.1 711 3UTR 100% 15.000 10.500 N DNAJB14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_938772.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04159 pDONR223 100% 58.8% None 1_142del;875_990del;1079_1080ins316 n/a
2 ccsbBroad304_04159 pLX_304 0% 58.8% V5 1_142del;875_990del;1079_1080ins316 n/a
3 TRCN0000472243 TCTCTCTGACATTTTGCCTTCCTT pLX_317 39.2% 58.8% V5 1_142del;875_990del;1079_1080ins316 n/a
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