Construct: ORF TRCN0000472243
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005126.1_s317c1
- Derived from:
- ccsbBroadEn_04159
- DNA Barcode:
- TCTCTCTGACATTTTGCCTTCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJB14 (79982)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472243
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79982 | DNAJB14 | DnaJ heat shock protein fam... | NM_001031723.4 | 100% | 100% | |
| 2 | human | 79982 | DNAJB14 | DnaJ heat shock protein fam... | NM_001278310.2 | 82.3% | 74.5% | 0_1ins29;103_104ins172 |
| 3 | human | 79982 | DNAJB14 | DnaJ heat shock protein fam... | XM_011532262.3 | 76.1% | 71.7% | (many diffs) |
| 4 | human | 79982 | DNAJB14 | DnaJ heat shock protein fam... | XM_017008628.2 | 74.5% | 71.6% | (many diffs) |
| 5 | human | 79982 | DNAJB14 | DnaJ heat shock protein fam... | XR_938772.2 | 58.8% | 1_142del;875_990del;1079_1080ins316 | |
| 6 | human | 79982 | DNAJB14 | DnaJ heat shock protein fam... | NM_001278311.2 | 28.4% | 26.6% | (many diffs) |
| 7 | human | 79982 | DNAJB14 | DnaJ heat shock protein fam... | XR_001741327.1 | 18.6% | 1_116del;422_477del;1310_6092del | |
| 8 | mouse | 70604 | Dnajb14 | DnaJ heat shock protein fam... | NM_001033155.1 | 85.9% | 93.4% | (many diffs) |
| 9 | mouse | 70604 | Dnajb14 | DnaJ heat shock protein fam... | XM_006502055.2 | 74.5% | 81.2% | (many diffs) |
| 10 | mouse | 70604 | Dnajb14 | DnaJ heat shock protein fam... | XM_006502056.2 | 71.7% | 71.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1203
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggggaacagg gatgaggctg agaaatgtgt cgagatcgcc cgggaggccc 121 tgaacgccgg caaccgcgag aaggcccagc gcttcctgca gaaggccgag aagctctacc 181 cactgccctc ggcccgcgca ctattggaaa taattatgaa aaatggaagc acggctggaa 241 atagccctca ttgccgaaaa ccatcaggta gtggcgatca aagcaagcct aattgcacaa 301 aggacagcac atctggtagt ggtgaaggtg gaaaaggcta taccaaagac caagtagatg 361 gagttctcag cataaacaaa tgtaaaaatt actatgaagt acttggagtt acgaaagatg 421 ctggtgatga agatttgaaa aaagcttata gaaagcttgc tttgaagttt catccagaca 481 aaaaccatgc acctggagca acagatgctt ttaaaaagat tggaaatgct tatgctgttt 541 taagtaatcc agaaaagcga aaacagtatg acctcacggg caatgaagaa caagcatgta 601 accaccaaaa caatggcaga tttaatttcc atagaggttg tgaagctgat ataactccag 661 aagacttgtt taatatattt tttgggggtg gatttccttc aggtagtgta cattcttttt 721 caaatggaag agctggttat agccaacaac atcagcatcG ACATAGTGGA CATGAAAGAG 781 AAGAGGAAAG AGGAGATGGA GGTTTTTCTG TGTTTATCCA GCTGATGCCC ATAATTGTAT 841 TGATCCTCGT GTCATTATTA AGCCAGTTGA TGGTCTCTAA TCCTCCTTAT TCCTTATATC 901 CCAGATCTGG AACTGGGCAA ACTATTAAAA TGCAAACAGA AAACTTGGGT GTTGTTTATT 961 ATGTCAACAA GGACTTCAAA AATGAATATA AAGGAATGTT ATTACAAAAG GTAGAAAAGA 1021 GTGTGGAGGA AGATTATGTG ACTAATATTC GAAATAACTG CTGGAAAGAA AGACAACAAA 1081 AAACAGATAT GCAGTATGCA GCAAAAGTAT ACCGTGATGA TCGACTCCGA AGGAAGGCAG 1141 ATGCCTTGAG CATGGACAAC TGTAAAGAAT TAGAGCGGCT TACCAGTCTT TATAAAGGAG 1201 GATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGATC TCTCTGACAT TTTGCCTTCC TTACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt