Transcript: Human XR_943522.3

PREDICTED: Homo sapiens zinc finger and BTB domain containing 25 (ZBTB25), transcript variant X7, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
ZBTB25 (7597)
Length:
1784
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_943522.3
NBCI Gene record:
ZBTB25 (7597)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_943522.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000021294 GCCGACTACCTTTCTCACATT pLKO.1 1049 3UTR 100% 4.950 6.930 N ZBTB25 n/a
2 TRCN0000275885 GCCGACTACCTTTCTCACATT pLKO_005 1049 3UTR 100% 4.950 6.930 N ZBTB25 n/a
3 TRCN0000021298 CCAACCTGACATATTCAGCTA pLKO.1 943 3UTR 100% 2.640 3.696 N ZBTB25 n/a
4 TRCN0000285454 CCAACCTGACATATTCAGCTA pLKO_005 943 3UTR 100% 2.640 3.696 N ZBTB25 n/a
5 TRCN0000021297 GCTTCCATTCTGGAAAGTAAT pLKO.1 1550 3UTR 100% 13.200 9.240 N ZBTB25 n/a
6 TRCN0000275884 GCTTCCATTCTGGAAAGTAAT pLKO_005 1550 3UTR 100% 13.200 9.240 N ZBTB25 n/a
7 TRCN0000275946 TTGGAGGAAGGGATTCGATTT pLKO_005 1022 3UTR 100% 10.800 6.480 N ZBTB25 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_943522.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01803 pDONR223 100% 50.8% None (many diffs) n/a
2 ccsbBroad304_01803 pLX_304 0% 50.8% V5 (many diffs) n/a
3 TRCN0000481016 CAGCGCAGAGCACTAAGCAGGTTG pLX_317 31.6% 50.8% V5 (many diffs) n/a
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