Construct: ORF BRDN0000556276
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- n/a
- Derived from:
- BRDN0000464774
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- eGFP (eGFP)
Vector Information:
- Vector Backbone:
- pLXI_401
- Pol II Cassette 1:
- TRE-Gateway-No Tag
- Pol II Cassette 2:
- PGK-PuroR-2A-rtTA-VP16
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | CONTROL | eGFP | eGFP | Hahn Lab eGFP | eGFP.1 | 100% | 100% | |
| 2 | CONTROL | GFP | GFP | clonetechGfp | clonetechGfp.1 | 99.7% | 99.5% | 93C>T;491T>C |
| 3 | CONTROL | BFP | BFP | Hahn Lab BFP | BFP.1 | 99.1% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 792
- ORF length:
- 717
- Sequence:
-
1 gctcgtttag tgaaccgtca gatcgcctgg agaattggct agcatcaaca agtttgtaca 61 aaaaagttgg caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 121 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtctggc gagggcgagg 181 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 241 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 301 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 361 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 421 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 481 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 541 acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggca 601 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 661 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 721 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 781 agctgtacaa gtccccaact ttcttgtaca aagtggttta gtaatgaacc ggtccaccac 841 caccaccacc actaaggatc cggggttggg gttgcgc