Construct: sgRNA BRDN0001144965
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTTGACTCAGGTCACCTCA
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- ACVR1B (91)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000012.12 | 12 | 51976333 | + | ACVR1B | NNNTGACTCAGGTCACCTCA | NGG | 0 | 1.0 | Tier I |
2 | NC_000001.11 | 1 | 109628633 | + | AMPD2 | NNNTGACTCAGCTCACCTCA | NGT | 2 | 0.0085 | Tier I |
3 | NC_000009.12 | 9 | 133737029 | - | SARDH | NNNTGACCCTGGTCACCTCA | NGG | 2 | 0.8824 | Tier II |
4 | NC_000023.11 | X | 152116652 | - | LOC100533997 | NNNTGACTCAGGTCAACACA | NGG | 2 | 0.6667 | Tier II |
5 | NC_000023.11 | X | 152716005 | + | MAGEA2B | NNNTGACTCAGGTCAACACA | NGG | 2 | 0.6667 | Tier II |
6 | NC_000023.11 | X | 152735196 | + | MAGEA12 | NNNTGACTCAGGTCAACACA | NGG | 2 | 0.6667 | Tier II |
7 | NC_000023.11 | X | 152752466 | - | MAGEA2 | NNNTGACTCAGGTCAACACA | NGG | 2 | 0.6667 | Tier II |
8 | NC_000023.11 | X | 151922574 | + | MAGEA4 | NNNTGACTCAGGTCACCACA | NGG | 1 | 0.6667 | Tier II |
9 | NC_000007.14 | 7 | 1122100 | - | C7orf50 | NNNTGACTCTGGTTACCTCA | NGG | 2 | 0.6471 | Tier II |
10 | NC_000002.12 | 2 | 11721688 | + | LPIN1 | NNNTGACTTGGGTCACCTCA | NGG | 2 | 0.4861 | Tier II |
11 | NC_000009.12 | 9 | 127029826 | + | RALGPS1 | NNNTGTCTCAGCTCACCTCA | NGG | 2 | 0.3782 | Tier II |
12 | NC_000011.10 | 11 | 120954563 | + | GRIK4 | NNNACACTCAGGTCACCTCA | NGG | 2 | 0.3741 | Tier II |
13 | NC_000005.10 | 5 | 107605986 | + | EFNA5 | NNNTCACTCAGGTCACTTCA | NGG | 2 | 0.3667 | Tier II |
14 | NC_000011.10 | 11 | 110436929 | - | FDX1 | NNNTGACTCAGGTAACCTCA | NGG | 1 | 0.35 | Tier II |
15 | NC_000005.10 | 5 | 149843845 | + | PPARGC1B | NNNTGACTCACGTCACCACA | NGG | 2 | 0.2857 | Tier II |
16 | NC_000018.10 | 18 | 36709487 | - | FHOD3 | NNNTGACCCAGGTCACCTCA | NAG | 2 | 0.2593 | Tier II |
17 | NC_000007.14 | 7 | 133315068 | - | EXOC4 | NNNTGACTCAGCTCACATCA | NGG | 2 | 0.2471 | Tier II |
18 | NC_000017.11 | 17 | 35825124 | - | TAF15 | NNNTTTCTCAGGTCACCTCA | NGG | 2 | 0.2143 | Tier II |
19 | NC_000002.12 | 2 | 110747616 | - | ACOXL | NNNTGACTCAGGTCATCACA | NGG | 2 | 0.2051 | Tier II |
20 | NC_000002.12 | 2 | 4107976 | - | LOC105373397 | NNNTGACTCAGTTCACTTCA | NGG | 2 | 0.1795 | Tier II |
21 | NC_000008.11 | 8 | 54709161 | - | RP1 | NNNTGACTCTGGTCTCCTCA | NGG | 2 | 0.1765 | Tier II |
22 | NC_000006.12 | 6 | 1698581 | - | GMDS | NNNTGACTCAGCTCACCTCC | NGG | 2 | 0.1203 | Tier II |
23 | NC_000018.10 | 18 | 23291509 | + | TMEM241 | NNNTGACTGAGGTCACCTGA | NGG | 2 | 0.0774 | Tier II |
24 | NC_000011.10 | 11 | 4022843 | + | STIM1 | NNNTGACTCAGGGCCCCTCA | NGG | 2 | 0.0711 | Tier II |
25 | NC_000006.12 | 6 | 129061275 | + | LAMA2 | NNNTGACTCAGGTCATCTCC | NGG | 2 | 0.0699 | Tier II |
26 | NC_000017.11 | 17 | 1644662 | + | SCARF1 | NNNTGACTCAGGTCATCTCC | NGG | 2 | 0.0699 | Tier II |
27 | NC_000001.11 | 1 | 41037697 | - | SCMH1 | NNNTGACTAAGGTCACCTCA | NGA | 2 | 0.0595 | Tier II |
28 | NC_000006.12 | 6 | 138896598 | + | ECT2L | NNNTGACTCAGGTCAGCTCA | NAG | 2 | 0.0399 | Tier II |
29 | NC_000001.11 | 1 | 3353650 | + | PRDM16 | NNNTGACTCAGGCCACCTCA | NTG | 2 | 0.0308 | Tier II |
30 | NC_000014.9 | 14 | 102263038 | + | MOK | NNNTGACTGAGGTCACCTCA | NGC | 2 | 0.0138 | Tier II |
31 | NC_000001.11 | 1 | 109628612 | + | AMPD2 | NNNTGACTCAGCTCACCTCA | NGT | 2 | 0.0085 | Tier II |
32 | NC_000012.12 | 12 | 119179798 | + | HSPB8 | NNNTGACACAGGTGACCTCA | NGG | 2 | 0.0 | Tier II |
33 | NC_000006.12 | 6 | 130343226 | - | SAMD3 | NNNTGACTCAGGGGACCTCA | NGG | 2 | 0.0 | Tier II |
34 | NC_000023.11 | X | 152116652 | - | MAGEA5P | NNNTGACTCAGGTCAACACA | NGG | 2 | 0.6667 | Tier III |
35 | NC_000023.11 | X | 152116652 | - | LOC105373369 | NNNTGACTCAGGTCAACACA | NGG | 2 | 0.6667 | Tier III |
36 | NC_000023.11 | X | 152735196 | + | CSAG4 | NNNTGACTCAGGTCAACACA | NGG | 2 | 0.6667 | Tier III |
37 | NC_000007.14 | 7 | 1122100 | - | LOC102723758 | NNNTGACTCTGGTTACCTCA | NGG | 2 | 0.6471 | Tier III |
38 | NC_000002.12 | 2 | 11721688 | + | LOC101929752 | NNNTGACTTGGGTCACCTCA | NGG | 2 | 0.4861 | Tier III |
39 | NC_000011.10 | 11 | 120954563 | + | LOC101929208 | NNNACACTCAGGTCACCTCA | NGG | 2 | 0.3741 | Tier III |
40 | NC_000007.14 | 7 | 133315068 | - | RPS15AP23 | NNNTGACTCAGCTCACATCA | NGG | 2 | 0.2471 | Tier III |
41 | NC_000011.10 | 11 | 112709910 | + | LOC105369496 | NNNTGACTCAGGTCACCACA | NAG | 2 | 0.1728 | Tier III |
42 | NC_000011.10 | 11 | 112338992 | - | LINC02762 | NNNGGACTCAGGTCACCTCA | NAG | 2 | 0.162 | Tier III |
43 | NC_000011.10 | 11 | 112338992 | - | LOC107984388 | NNNGGACTCAGGTCACCTCA | NAG | 2 | 0.162 | Tier III |
44 | NC_000001.11 | 1 | 41037697 | - | SLFNL1-AS1 | NNNTGACTAAGGTCACCTCA | NGA | 2 | 0.0595 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000069.6 | 3 | 146604970 | + | Uox | NNNTGACTCTGGTTACCTCA | NGG | 2 | 0.6471 | Tier II |
2 | NC_000069.6 | 3 | 146604970 | + | Dnase2b | NNNTGACTCTGGTTACCTCA | NGG | 2 | 0.6471 | Tier II |
3 | NC_000081.6 | 15 | 82161878 | + | Srebf2 | NNNTGAAACAGGTCACCTCA | NGG | 2 | 0.6 | Tier II |
4 | NC_000074.6 | 8 | 16101541 | - | Csmd1 | NNNTGACACAGGTCACATCA | NGG | 2 | 0.3733 | Tier II |
5 | NC_000074.6 | 8 | 104227971 | - | Tk2 | NNNTGCATCAGGTCACCTCA | NGG | 2 | 0.3409 | Tier II |
6 | NC_000071.6 | 5 | 33609597 | - | Fam53a | NNNTGTCTCAGGTCACCCCA | NGG | 2 | 0.3061 | Tier II |
7 | NC_000069.6 | 3 | 142729325 | - | Kyat3 | NNNAGACTGAGGTCACCTCA | NGG | 2 | 0.2948 | Tier II |
8 | NC_000072.6 | 6 | 37397405 | + | Creb3l2 | NNNTGAGTCAGCTCACCTCA | NGG | 2 | 0.2491 | Tier II |
9 | NC_000068.7 | 2 | 54633198 | + | Galnt13 | NNNGGACTCAGGTAACCTCA | NGG | 2 | 0.2188 | Tier II |
10 | NC_000068.7 | 2 | 125713595 | + | Shc4 | NNNTGAATCAGGGCACCTCA | NGG | 2 | 0.1957 | Tier II |
11 | NC_000083.6 | 17 | 24152485 | + | Pdpk1 | NNNTGACTCAGCTAACCTCA | NGG | 2 | 0.1853 | Tier II |
12 | NC_000067.6 | 1 | 123721937 | - | Dpp10 | NNNTGACTCAGGTTACCTCC | NGG | 2 | 0.1667 | Tier II |
13 | NC_000083.6 | 17 | 62819399 | + | Efna5 | NNNTTACTCAGGTCACTTCA | NGG | 2 | 0.14 | Tier II |
14 | NC_000071.6 | 5 | 116239877 | + | Ccdc60 | NNNTGCCTCAGGGCACCTCA | NGG | 2 | 0.1186 | Tier II |
15 | NC_000076.6 | 10 | 94122229 | - | Fgd6 | NNNTGACTCAGTTCAGCTCA | NGG | 2 | 0.0592 | Tier II |
16 | NC_000075.6 | 9 | 49653131 | - | Ncam1 | NNNTGACTCAGGTAAGCTCA | NGG | 2 | 0.0538 | Tier II |
17 | NC_000080.6 | 14 | 27727436 | + | Erc2 | NNNGGACTCAGGTCACCTCA | NGA | 2 | 0.0434 | Tier II |
18 | NC_000068.7 | 2 | 125472145 | - | Fbn1 | NNNTGACTCAGGCCACCTCA | NTG | 2 | 0.0308 | Tier II |
19 | NC_000078.6 | 12 | 70865937 | + | Frmd6 | NNNTGACTCAGGTCACCTCG | NTG | 2 | 0.0298 | Tier II |
20 | NC_000073.6 | 7 | 105568036 | + | Apbb1 | NNNTGACCCAGGTCACCTCA | NGT | 2 | 0.0161 | Tier II |
21 | NC_000074.6 | 8 | 123527391 | + | Gas8 | NNNTGACTCAGGGCACCTCA | NTG | 2 | 0.0102 | Tier II |
22 | NC_000078.6 | 12 | 3580823 | - | Dtnb | NNNTGACTCAGGTCACCTCT | NGT | 2 | 0.0097 | Tier II |
23 | NC_000074.6 | 8 | 41239609 | + | Pcm1 | NNNTGACTCAGGTCACCGCA | NGC | 2 | 0.0074 | Tier II |
24 | NC_000071.6 | 5 | 140129821 | + | Mad1l1 | NNNTGACTCAGGTGACCACA | NGG | 2 | 0.0 | Tier II |
25 | NC_000072.6 | 6 | 72026744 | - | 9130221F21Rik | NNNTCACTCAGGTCACCTCT | NGG | 2 | 0.4714 | Tier III |
26 | NC_000078.6 | 12 | 25155707 | - | Gm36287 | NNNTGACTCAAGGCACCTCA | NGG | 2 | 0.2609 | Tier III |
27 | NC_000073.6 | 7 | 98726362 | - | Gm19656 | NNNTGATTCAGGTCCCCTCA | NGG | 2 | 0.2216 | Tier III |
28 | NC_000078.6 | 12 | 87863530 | - | Gm1995 | NNNTGACTCGGGTCACCTCC | NGG | 2 | 0.1263 | Tier III |
29 | NC_000078.6 | 12 | 88284974 | + | Gm7104 | NNNTGACTCGGGTCACCTCC | NGG | 2 | 0.1263 | Tier III |