Construct: sgRNA BRDN0001144965

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTTGACTCAGGTCACCTCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACVR1B (91)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76680

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 51976333 + ACVR1B NNNTGACTCAGGTCACCTCA NGG 0 1.0 Tier I
2 NC_000001.11 1 109628633 + AMPD2 NNNTGACTCAGCTCACCTCA NGT 2 0.0085 Tier I
3 NC_000009.12 9 133737029 - SARDH NNNTGACCCTGGTCACCTCA NGG 2 0.8824 Tier II
4 NC_000023.11 X 152116652 - LOC100533997 NNNTGACTCAGGTCAACACA NGG 2 0.6667 Tier II
5 NC_000023.11 X 152716005 + MAGEA2B NNNTGACTCAGGTCAACACA NGG 2 0.6667 Tier II
6 NC_000023.11 X 152735196 + MAGEA12 NNNTGACTCAGGTCAACACA NGG 2 0.6667 Tier II
7 NC_000023.11 X 152752466 - MAGEA2 NNNTGACTCAGGTCAACACA NGG 2 0.6667 Tier II
8 NC_000023.11 X 151922574 + MAGEA4 NNNTGACTCAGGTCACCACA NGG 1 0.6667 Tier II
9 NC_000007.14 7 1122100 - C7orf50 NNNTGACTCTGGTTACCTCA NGG 2 0.6471 Tier II
10 NC_000002.12 2 11721688 + LPIN1 NNNTGACTTGGGTCACCTCA NGG 2 0.4861 Tier II
11 NC_000009.12 9 127029826 + RALGPS1 NNNTGTCTCAGCTCACCTCA NGG 2 0.3782 Tier II
12 NC_000011.10 11 120954563 + GRIK4 NNNACACTCAGGTCACCTCA NGG 2 0.3741 Tier II
13 NC_000005.10 5 107605986 + EFNA5 NNNTCACTCAGGTCACTTCA NGG 2 0.3667 Tier II
14 NC_000011.10 11 110436929 - FDX1 NNNTGACTCAGGTAACCTCA NGG 1 0.35 Tier II
15 NC_000005.10 5 149843845 + PPARGC1B NNNTGACTCACGTCACCACA NGG 2 0.2857 Tier II
16 NC_000018.10 18 36709487 - FHOD3 NNNTGACCCAGGTCACCTCA NAG 2 0.2593 Tier II
17 NC_000007.14 7 133315068 - EXOC4 NNNTGACTCAGCTCACATCA NGG 2 0.2471 Tier II
18 NC_000017.11 17 35825124 - TAF15 NNNTTTCTCAGGTCACCTCA NGG 2 0.2143 Tier II
19 NC_000002.12 2 110747616 - ACOXL NNNTGACTCAGGTCATCACA NGG 2 0.2051 Tier II
20 NC_000002.12 2 4107976 - LOC105373397 NNNTGACTCAGTTCACTTCA NGG 2 0.1795 Tier II
21 NC_000008.11 8 54709161 - RP1 NNNTGACTCTGGTCTCCTCA NGG 2 0.1765 Tier II
22 NC_000006.12 6 1698581 - GMDS NNNTGACTCAGCTCACCTCC NGG 2 0.1203 Tier II
23 NC_000018.10 18 23291509 + TMEM241 NNNTGACTGAGGTCACCTGA NGG 2 0.0774 Tier II
24 NC_000011.10 11 4022843 + STIM1 NNNTGACTCAGGGCCCCTCA NGG 2 0.0711 Tier II
25 NC_000006.12 6 129061275 + LAMA2 NNNTGACTCAGGTCATCTCC NGG 2 0.0699 Tier II
26 NC_000017.11 17 1644662 + SCARF1 NNNTGACTCAGGTCATCTCC NGG 2 0.0699 Tier II
27 NC_000001.11 1 41037697 - SCMH1 NNNTGACTAAGGTCACCTCA NGA 2 0.0595 Tier II
28 NC_000006.12 6 138896598 + ECT2L NNNTGACTCAGGTCAGCTCA NAG 2 0.0399 Tier II
29 NC_000001.11 1 3353650 + PRDM16 NNNTGACTCAGGCCACCTCA NTG 2 0.0308 Tier II
30 NC_000014.9 14 102263038 + MOK NNNTGACTGAGGTCACCTCA NGC 2 0.0138 Tier II
31 NC_000001.11 1 109628612 + AMPD2 NNNTGACTCAGCTCACCTCA NGT 2 0.0085 Tier II
32 NC_000012.12 12 119179798 + HSPB8 NNNTGACACAGGTGACCTCA NGG 2 0.0 Tier II
33 NC_000006.12 6 130343226 - SAMD3 NNNTGACTCAGGGGACCTCA NGG 2 0.0 Tier II
34 NC_000023.11 X 152116652 - MAGEA5P NNNTGACTCAGGTCAACACA NGG 2 0.6667 Tier III
35 NC_000023.11 X 152116652 - LOC105373369 NNNTGACTCAGGTCAACACA NGG 2 0.6667 Tier III
36 NC_000023.11 X 152735196 + CSAG4 NNNTGACTCAGGTCAACACA NGG 2 0.6667 Tier III
37 NC_000007.14 7 1122100 - LOC102723758 NNNTGACTCTGGTTACCTCA NGG 2 0.6471 Tier III
38 NC_000002.12 2 11721688 + LOC101929752 NNNTGACTTGGGTCACCTCA NGG 2 0.4861 Tier III
39 NC_000011.10 11 120954563 + LOC101929208 NNNACACTCAGGTCACCTCA NGG 2 0.3741 Tier III
40 NC_000007.14 7 133315068 - RPS15AP23 NNNTGACTCAGCTCACATCA NGG 2 0.2471 Tier III
41 NC_000011.10 11 112709910 + LOC105369496 NNNTGACTCAGGTCACCACA NAG 2 0.1728 Tier III
42 NC_000011.10 11 112338992 - LINC02762 NNNGGACTCAGGTCACCTCA NAG 2 0.162 Tier III
43 NC_000011.10 11 112338992 - LOC107984388 NNNGGACTCAGGTCACCTCA NAG 2 0.162 Tier III
44 NC_000001.11 1 41037697 - SLFNL1-AS1 NNNTGACTAAGGTCACCTCA NGA 2 0.0595 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000069.6 3 146604970 + Uox NNNTGACTCTGGTTACCTCA NGG 2 0.6471 Tier II
2 NC_000069.6 3 146604970 + Dnase2b NNNTGACTCTGGTTACCTCA NGG 2 0.6471 Tier II
3 NC_000081.6 15 82161878 + Srebf2 NNNTGAAACAGGTCACCTCA NGG 2 0.6 Tier II
4 NC_000074.6 8 16101541 - Csmd1 NNNTGACACAGGTCACATCA NGG 2 0.3733 Tier II
5 NC_000074.6 8 104227971 - Tk2 NNNTGCATCAGGTCACCTCA NGG 2 0.3409 Tier II
6 NC_000071.6 5 33609597 - Fam53a NNNTGTCTCAGGTCACCCCA NGG 2 0.3061 Tier II
7 NC_000069.6 3 142729325 - Kyat3 NNNAGACTGAGGTCACCTCA NGG 2 0.2948 Tier II
8 NC_000072.6 6 37397405 + Creb3l2 NNNTGAGTCAGCTCACCTCA NGG 2 0.2491 Tier II
9 NC_000068.7 2 54633198 + Galnt13 NNNGGACTCAGGTAACCTCA NGG 2 0.2188 Tier II
10 NC_000068.7 2 125713595 + Shc4 NNNTGAATCAGGGCACCTCA NGG 2 0.1957 Tier II
11 NC_000083.6 17 24152485 + Pdpk1 NNNTGACTCAGCTAACCTCA NGG 2 0.1853 Tier II
12 NC_000067.6 1 123721937 - Dpp10 NNNTGACTCAGGTTACCTCC NGG 2 0.1667 Tier II
13 NC_000083.6 17 62819399 + Efna5 NNNTTACTCAGGTCACTTCA NGG 2 0.14 Tier II
14 NC_000071.6 5 116239877 + Ccdc60 NNNTGCCTCAGGGCACCTCA NGG 2 0.1186 Tier II
15 NC_000076.6 10 94122229 - Fgd6 NNNTGACTCAGTTCAGCTCA NGG 2 0.0592 Tier II
16 NC_000075.6 9 49653131 - Ncam1 NNNTGACTCAGGTAAGCTCA NGG 2 0.0538 Tier II
17 NC_000080.6 14 27727436 + Erc2 NNNGGACTCAGGTCACCTCA NGA 2 0.0434 Tier II
18 NC_000068.7 2 125472145 - Fbn1 NNNTGACTCAGGCCACCTCA NTG 2 0.0308 Tier II
19 NC_000078.6 12 70865937 + Frmd6 NNNTGACTCAGGTCACCTCG NTG 2 0.0298 Tier II
20 NC_000073.6 7 105568036 + Apbb1 NNNTGACCCAGGTCACCTCA NGT 2 0.0161 Tier II
21 NC_000074.6 8 123527391 + Gas8 NNNTGACTCAGGGCACCTCA NTG 2 0.0102 Tier II
22 NC_000078.6 12 3580823 - Dtnb NNNTGACTCAGGTCACCTCT NGT 2 0.0097 Tier II
23 NC_000074.6 8 41239609 + Pcm1 NNNTGACTCAGGTCACCGCA NGC 2 0.0074 Tier II
24 NC_000071.6 5 140129821 + Mad1l1 NNNTGACTCAGGTGACCACA NGG 2 0.0 Tier II
25 NC_000072.6 6 72026744 - 9130221F21Rik NNNTCACTCAGGTCACCTCT NGG 2 0.4714 Tier III
26 NC_000078.6 12 25155707 - Gm36287 NNNTGACTCAAGGCACCTCA NGG 2 0.2609 Tier III
27 NC_000073.6 7 98726362 - Gm19656 NNNTGATTCAGGTCCCCTCA NGG 2 0.2216 Tier III
28 NC_000078.6 12 87863530 - Gm1995 NNNTGACTCGGGTCACCTCC NGG 2 0.1263 Tier III
29 NC_000078.6 12 88284974 + Gm7104 NNNTGACTCGGGTCACCTCC NGG 2 0.1263 Tier III
Download CSV

Other clones with same target sequence:

(none)