Construct: sgRNA BRDN0001144977

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGAAATTGCCTCACATGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PHKA1 (5255)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77838

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000023.11 X 72652587 - PHKA1 NNNAAATTGCCTCACATGTG NGG 0 1.0 Tier I
2 NC_000011.10 11 125644267 - CHEK1 NNNAAATTCCCTCACCTGTG NGG 2 0.0 Tier I
3 NC_000013.11 13 113044220 - MCF2L NNNAAAATGCCTCACATGTA NGG 2 0.8203 Tier II
4 NC_000007.14 7 80621555 + CD36 NNNAAGTTGTCTCACATGTG NGG 2 0.6723 Tier II
5 NC_000022.11 22 21839989 - MAPK1 NNNAAATGGCATCACATGTG NGG 2 0.55 Tier II
6 NC_000002.12 2 233108463 + INPP5D NNNAAATTGCATCACATATG NGG 2 0.5192 Tier II
7 NC_000001.11 1 96740509 + PTBP2 NNNAAATTGCATCACATTTG NGG 2 0.5 Tier II
8 NC_000020.11 20 37758422 + CTNNBL1 NNNAAACTGCCTTACATGTG NGG 2 0.4812 Tier II
9 NC_000015.10 15 98998368 - PGPEP1L NNNACAATGCCTCACATGTG NGG 2 0.4375 Tier II
10 NC_000023.11 X 72361868 + HDAC8 NNNAAATGCCCTCACATGTG NGG 2 0.3949 Tier II
11 NC_000013.11 13 92367755 - GPC5 NNNAAATTGCCTTACAAGTG NGG 2 0.3733 Tier II
12 NC_000010.11 10 76257868 + LRMDA NNNAAATGGCCTCACATGTC NGG 2 0.3143 Tier II
13 NC_000021.9 21 44033432 + TRAPPC10 NNNAAATTGCTTAACATGTG NGG 2 0.1183 Tier II
14 NC_000015.10 15 29831374 - TJP1 NNNCAATTGCCTCACATGGG NGG 2 0.0882 Tier II
15 NC_000011.10 11 56611517 - OR5M1 NNNAAATTGCCTCATATGTT NGG 2 0.0467 Tier II
16 NC_000005.10 5 13707338 - DNAH5 NNNAAATTGCCTCACATGTG NGC 1 0.0222 Tier II
17 NC_000013.11 13 94459357 + DCT NNNAAATTGCCTCAAATGTG NGT 2 0.0036 Tier II
18 NC_000019.10 19 42000507 + GRIK5 NNNAAATTGCCTCACAGGTG NGC 2 0.0026 Tier II
19 NC_000008.11 8 42961116 + HOOK3 NNNAAATTCCCTCACTTGTG NGG 2 0.0 Tier II
20 NC_000019.10 19 23259442 - IPO5P1 NNNTAATTGCCTCTCATGTG NGG 2 0.3394 Tier III
21 NC_000007.14 7 35349729 - LOC401324 NNNAAATTGCCTCTCATCTG NGG 2 0.254 Tier III
22 NC_000009.12 9 70465860 + KLF9-DT NNNAAATTGCATCACATGTG NGC 2 0.0167 Tier III
23 NC_000005.10 5 119938218 - LOC105379144 NNNAAATTGCCTCAATTGTG NGG 2 0.0 Tier III
24 NC_000002.12 2 21948188 - LOC124908056 NNNAAATTGCCTGACCTGTG NGG 2 0.0 Tier III
25 NC_000009.12 9 11381827 + LOC105375974 NNNAAATTGCCTTACTTGTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000086.7 X 102597445 - Phka1 NNNAAATTGCCTCACATGTG NGG 0 1.0 Tier I
2 NC_000087.7 Y 726184 - Zfy1 NNNAAATTTCCTCACATTTG NGG 2 0.3556 Tier I
3 NC_000087.7 Y 2107071 - Zfy2 NNNAAATTTCCTCACATTTG NGG 2 0.3556 Tier I
4 NC_000085.6 19 48789736 - Sorcs3 NNNAAAAAGCCTCACATGTG NGG 2 0.7 Tier II
5 NC_000072.6 6 145114169 - Lrmp NNNAAACTGACTCACATGTG NGG 2 0.5958 Tier II
6 NC_000071.6 5 129165309 + Adgrd1 NNNGAAGTGCCTCACATGTG NGG 2 0.3676 Tier II
7 NC_000084.6 18 37329226 - Gm37013 NNNAAATTCCTTCACATGTG NGG 2 0.1657 Tier II
8 NC_000084.6 18 37329226 - Gm38666 NNNAAATTCCTTCACATGTG NGG 2 0.1657 Tier II
9 NC_000084.6 18 37329226 - Gm38667 NNNAAATTCCTTCACATGTG NGG 2 0.1657 Tier II
10 NC_000068.7 2 120717203 + Cdan1 NNNCACTTGCCTCACATGTG NGG 2 0.1604 Tier II
11 NC_000068.7 2 120717203 + Stard9 NNNCACTTGCCTCACATGTG NGG 2 0.1604 Tier II
12 NC_000077.6 11 104371202 - Kansl1 NNNAAATTGCCTCAAATTTG NGG 2 0.1481 Tier II
13 NC_000086.7 X 134549787 + Btk NNNAAAGTGCCTCAAATGTG NGG 2 0.1307 Tier II
14 NC_000068.7 2 115983495 - Meis2 NNNAAATTGCCTCACATGTG NGA 1 0.0694 Tier II
15 NC_000084.6 18 65882029 - Grp NNNAAATTGCCACAGATGTG NGG 2 0.04 Tier II
16 NC_000075.6 9 67905468 + Vps13c NNNAAATTGCCTCACATGTT NTG 2 0.0273 Tier II
17 NC_000084.6 18 65882029 - Gm30094 NNNAAATTGCCACAGATGTG NGG 2 0.04 Tier III
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Other clones with same target sequence:

(none)