Construct: sgRNA BRDN0001145051

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CAGGAGGTGGAATGAATGTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MYO3B (140469)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75568

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 170402870 + MYO3B NNNGAGGTGGAATGAATGTG NGG 0 1.0 Tier I
2 NC_000004.12 4 118887329 + SYNPO2 NNNGAGGAGGAATGAATGTA NGG 2 0.75 Tier II
3 NC_000011.10 11 32660045 + CCDC73 NNNGAGGTGGAATGAATGTT NGG 1 0.7 Tier II
4 NC_000013.11 13 29557750 + SLC7A1 NNNGAGGAGGAGTGAATGTG NGG 2 0.5778 Tier II
5 NC_000009.12 9 72773943 + TMC1 NNNGAGGTGTAATGGATGTG NGG 2 0.5281 Tier II
6 NC_000003.12 3 131950816 - CPNE4 NNNAAGTTGGAATGAATGTG NGG 2 0.5143 Tier II
7 NC_000010.11 10 115449373 - ATRNL1 NNNGAGGTGGAAAGAATATG NGG 2 0.4793 Tier II
8 NC_000002.12 2 120862238 + GLI2 NNNGACCTGGAATGAATGTG NGG 2 0.4687 Tier II
9 NC_000001.11 1 149984638 - OTUD7B NNNGAGGTGGAAAGAATTTG NGG 2 0.4615 Tier II
10 NC_000001.11 1 119799065 + REG4 NNNGAGGTTGAACGAATGTG NGG 2 0.4211 Tier II
11 NC_000006.12 6 52678290 + TMEM14A NNNGAGGTGGAAAGAAAGTG NGG 2 0.3692 Tier II
12 NC_000020.11 20 50853039 - BCAS4 NNNCAGGTGGAAAGAATGTG NGG 2 0.3665 Tier II
13 NC_000005.10 5 107948404 - FBXL17 NNNGTGATGGAATGAATGTG NGG 2 0.3636 Tier II
14 NC_000005.10 5 60498941 - PDE4D NNNTAGGTGGAATGAATGTG NGG 1 0.3636 Tier II
15 NC_000004.12 4 15359401 + C1QTNF7 NNNGAGGTGGAAAGAATGTC NGG 2 0.2967 Tier II
16 NC_000017.11 17 63445497 + CYB561 NNNAAGGTGGAATGAATGAG NGG 2 0.2571 Tier II
17 NC_000001.11 1 246294155 - SMYD3 NNNGAGGTGGAAGGAATGTA NGG 2 0.2446 Tier II
18 NC_000020.11 20 9603397 - PAK5 NNNGAGGTGGAATGAATGGA NGG 2 0.2344 Tier II
19 NC_000006.12 6 160242702 - SLC22A2 NNNGGGGTGGAATGCATGTG NGG 2 0.1964 Tier II
20 NC_000001.11 1 7434047 - CAMTA1 NNNGAGGTGGAAGGGATGTG NGG 2 0.1696 Tier II
21 NC_000008.11 8 40563157 - ZMAT4 NNNGAGGTGGAATGAGTTTG NGG 2 0.1282 Tier II
22 NC_000002.12 2 190657338 + NAB1 NNNGAGGTGGCATGAATGTG NAG 2 0.1037 Tier II
23 NC_000011.10 11 28366725 + METTL15 NNNGAGGAGGAATGAAGGTG NGG 2 0.0941 Tier II
24 NC_000004.12 4 185889823 - SORBS2 NNNGAGGTGGAATGAGTGTC NGG 2 0.0824 Tier II
25 NC_000002.12 2 190169410 + AKAP19 NNNGAGGTGGTCTGAATGTG NGG 2 0.081 Tier II
26 NC_000012.12 12 11685034 + ETV6 NNNGAGGTGGCATGAGTGTG NGG 2 0.0769 Tier II
27 NC_000012.12 12 123685969 - TCTN2 NNNGAGGTGGAATGAATGTT NGA 2 0.0486 Tier II
28 NC_000006.12 6 163164659 - PACRG NNNGAGGTGGAATGAATTTG NGA 2 0.0463 Tier II
29 NC_000007.14 7 71564632 - GALNT17 NNNGATGTGGAATGAATGTG NGA 2 0.0463 Tier II
30 NC_000013.11 13 69908019 + KLHL1 NNNGAGGTGGGATGAATGTG NTG 2 0.0253 Tier II
31 NC_000010.11 10 7272221 - SFMBT2 NNNGAGGTGAAATGAATGTG NGT 2 0.0151 Tier II
32 NC_000007.14 7 127770748 + SND1 NNNGTGGTGGAATGAATGTG NTG 2 0.0142 Tier II
33 NC_000016.10 16 73445866 + ZFHX3 NNNGAGGTGGAATAACTGTG NGG 2 0.0 Tier II
34 NC_000013.11 13 33392395 + STARD13 NNNGAGGTGGAATGAATGTG NTC 2 0.0 Tier II
35 NC_000001.11 1 234237243 + SLC35F3 NNNGAGGTGGAATGACTGTC NGG 2 0.0 Tier II
36 NC_000006.12 6 44918994 + SUPT3H NNNGAGGTGGAATGATTGTA NGG 2 0.0 Tier II
37 NC_000015.10 15 53480681 + LOC105370826 NNNAATGTGGAATGAATGTG NGG 2 0.6 Tier III
38 NC_000010.11 10 121195002 - LOC105378523 NNNGAGGTGTGATGAATGTG NGG 2 0.5281 Tier III
39 NC_000009.12 9 26409709 + LOC105375999 NNNAAGGTTGAATGAATGTG NGG 2 0.48 Tier III
40 NC_000005.10 5 60498941 - PART1 NNNTAGGTGGAATGAATGTG NGG 1 0.3636 Tier III
41 NC_000004.12 4 15359401 + C1QTNF7-AS1 NNNGAGGTGGAAAGAATGTC NGG 2 0.2967 Tier III
42 NC_000002.12 2 87495510 - CYTOR NNNGAGATGGAATGAATGAG NGG 2 0.2857 Tier III
43 NC_000002.12 2 87495510 - NCAL1 NNNGAGATGGAATGAATGAG NGG 2 0.2857 Tier III
44 NC_000002.12 2 111455337 + MIR4435-2HG NNNGAGATGGAATGAATGAG NGG 2 0.2857 Tier III
45 NC_000001.11 1 193358611 + LINC01031 NNNGAGGTGGAATGCATGTG NGG 1 0.2727 Tier III
46 NC_000015.10 15 62197616 + LOC107984784 NNNTAGGGGGAATGAATGTG NGG 2 0.2667 Tier III
47 NC_000020.11 20 9603397 - LOC105372523 NNNGAGGTGGAATGAATGGA NGG 2 0.2344 Tier III
48 NC_000002.12 2 65936638 - LINC02934 NNNGAGGTGGAATCAAAGTG NGG 2 0.2286 Tier III
49 NC_000005.10 5 1543328 + LOC124901161 NNNGAGGGGGAATGAATGGG NGG 2 0.1833 Tier III
50 NC_000005.10 5 1543387 + LOC124901161 NNNGAGGGGGAATGAATGGG NGG 2 0.1833 Tier III
51 NC_000005.10 5 1543403 + LOC124901161 NNNGAGGGGGAATGAATGGG NGG 2 0.1833 Tier III
52 NC_000005.10 5 1543550 + LOC124901161 NNNGAGGGGGAATGAATGGG NGG 2 0.1833 Tier III
53 NC_000008.11 8 122567132 + SMILR NNNGAGATGGAATGAATGTG NGT 2 0.0161 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000083.6 17 67821686 - Lama1 NNNGAGGTGGAATGGGTGTG NGG 2 0.125 Tier I
2 NC_000069.6 3 75636525 - Serpini1 NNNGAGGAGGAATGAATGTA NGG 2 0.75 Tier II
3 NC_000067.6 1 140584804 + Kcnt2 NNNGAGATGGAATGAATTTG NGG 2 0.6667 Tier II
4 NC_000071.6 5 121941490 - Cux2 NNNGAGGAGGAATGAACGTG NGG 2 0.5647 Tier II
5 NC_000077.6 11 36080180 + Tenm2 NNNGAGGTGGAAAAAATGTG NGG 2 0.5192 Tier II
6 NC_000068.7 2 96754643 + Lrrc4c NNNGAGGGGGAATGAATATG NGG 2 0.5077 Tier II
7 NC_000072.6 6 91989531 + Fgd5 NNNGGGGTGGAATGGATGTG NGG 2 0.468 Tier II
8 NC_000074.6 8 81666756 - Inpp4b NNNGAGGAGGAATGAAAGTG NGG 2 0.4267 Tier II
9 NC_000068.7 2 154995712 - a NNNGAGGAGGAATGAATCTG NGG 2 0.381 Tier II
10 NC_000071.6 5 19780666 - Magi2 NNNGAGGAGGAATGAATCTG NGG 2 0.381 Tier II
11 NC_000075.6 9 72049473 + Tcf12 NNNGTGGGGGAATGAATGTG NGG 2 0.2667 Tier II
12 NC_000076.6 10 103166821 + Lrriq1 NNNGTGGGGGAATGAATGTG NGG 2 0.2667 Tier II
13 NC_000086.7 X 60027831 + F9 NNNGAGATGGAAGGAATGTG NGG 2 0.2609 Tier II
14 NC_000082.6 16 11377922 - Snx29 NNNGAGGTGGCATGGATGTG NGG 2 0.26 Tier II
15 NC_000074.6 8 120449524 + Gse1 NNNAAGGTGGAAGGAATGTG NGG 2 0.2348 Tier II
16 NC_000083.6 17 56954103 + Acer1 NNNGAGGTGGAATGAATGGA NGG 2 0.2344 Tier II
17 NC_000085.6 19 4666463 - Gm960 NNNGAGGAGGAATGAATGTG NAG 2 0.2074 Tier II
18 NC_000082.6 16 89994803 - Tiam1 NNNGAGGTGGTATGAATTTG NGG 2 0.2051 Tier II
19 NC_000072.6 6 116168034 - Tmcc1 NNNGAGGTGGAATGAATTAG NGG 2 0.1905 Tier II
20 NC_000076.6 10 32396071 + Nkain2 NNNGAGGTGGAAAGAATGTG NAG 2 0.1795 Tier II
21 NC_000071.6 5 137787884 + Zcwpw1 NNNGAGGTGGAAGGGATGTG NGG 2 0.1696 Tier II
22 NC_000072.6 6 40327794 + Agk NNNGAGGTGGAAGGGATGTG NGG 2 0.1696 Tier II
23 NC_000077.6 11 80484134 + Myo1d NNNGAGGTGGAACGAGTGTG NGG 2 0.1518 Tier II
24 NC_000083.6 17 24182434 - Tbc1d24 NNNGAGTTGGACTGAATGTG NGG 2 0.1504 Tier II
25 NC_000081.6 15 12547717 + Pdzd2 NNNGAGGGGGAATGTATGTG NGG 2 0.1467 Tier II
26 NC_000070.6 4 130804703 - Sdc3 NNNGAGTTGGAATGAATGGG NGG 2 0.1429 Tier II
27 NC_000072.6 6 86625664 + Gm46963 NNNGAGGTGGGATGAGTGTG NGG 2 0.125 Tier II
28 NC_000067.6 1 74119996 + Tns1 NNNGAGGTGGAATCAATGAG NGG 2 0.1224 Tier II
29 NC_000067.6 1 166118793 + Dusp27 NNNGAGGTGAAATGAAGGTG NGG 2 0.1098 Tier II
30 NC_000069.6 3 127116318 - Ank2 NNNGAGGTGGAATCAATGGG NGG 2 0.1071 Tier II
31 NC_000084.6 18 68213354 - Ldlrad4 NNNGAGCTGGAATGAAGGTG NGG 2 0.0809 Tier II
32 NC_000077.6 11 109795729 + 1700012B07Rik NNNGTGGTGGAATGAGTGTG NGG 2 0.0699 Tier II
33 NC_000072.6 6 38890073 - Tbxas1 NNNGAGGTGGAATGGATGTG NGA 2 0.0451 Tier II
34 NC_000081.6 15 95967506 + Ano6 NNNGAGGTGAAATGAATGTG NTG 2 0.0364 Tier II
35 NC_000075.6 9 61399859 + Tle3 NNNGAGGTGGAATGAATGGG NGA 2 0.0174 Tier II
36 NC_000083.6 17 33584056 - Myo1f NNNGAGGTGGAATGAATTTG NGT 2 0.0108 Tier II
37 NC_000072.6 6 60985721 + Mmrn1 NNNGAGGTGGAAAGATTGTG NGG 2 0.0 Tier II
38 NC_000073.6 7 76815368 - Agbl1 NNNGAGGTGGAATGCTTGTG NGG 2 0.0 Tier II
39 NC_000072.6 6 68146866 - Igk NNNAAAGTGGAATGAATGTG NGG 2 0.9 Tier III
40 NC_000072.6 6 68297466 - Igk NNNAAGGTGGAATGAATGTG NGG 1 0.9 Tier III
41 NC_000067.6 1 140584804 + 4930590L20Rik NNNGAGATGGAATGAATTTG NGG 2 0.6667 Tier III
42 NC_000074.6 8 71044812 + Gm17576 NNNGAGGAGGAATGGATGTG NGG 2 0.52 Tier III
43 NC_000074.6 8 12236464 - Gm33029 NNNGCAGTGGAATGAATGTG NGG 2 0.5 Tier III
44 NC_000071.6 5 19780666 - Gm52765 NNNGAGGAGGAATGAATCTG NGG 2 0.381 Tier III
45 NC_000081.6 15 42771797 - Gm41314 NNNGAGGAGGAATGAATCTG NGG 2 0.381 Tier III
46 NC_000068.7 2 132624099 + AU019990 NNNTAGGTGTAATGAATGTG NGG 2 0.2955 Tier III
47 NC_000071.6 5 50124583 - Gm40300 NNNGGGGTGCAATGAATGTG NGG 2 0.288 Tier III
48 NC_000070.6 4 55604438 + Gm12506 NNNGAGGTGGAATTAATGTG NGA 2 0.0185 Tier III
49 NC_000085.6 19 27134882 + Gm35438 NNNGAGGTGGAAGGAATGTG NGC 2 0.0058 Tier III
50 NC_000068.7 2 73602879 + Chn1os3 NNNGAGGTGGAATGATCGTG NGG 2 0.0 Tier III
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Other clones with same target sequence:

(none)