Construct: sgRNA BRDN0001146809

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACCTCTCAAAGTATTCCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRKAR1A (5573)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77722

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000017.11 17 68515552 - PRKAR1A NNNTCTCAAAGTATTCCCTG NGG 0 1.0 Tier I
2 NC_000015.10 15 83754204 + ADAMTSL3 NNNTCTCAAAATGTTCCCTG NGG 2 0.6522 Tier II
3 NC_000001.11 1 92125455 - BTBD8 NNNTCTCAAAGTGTTCCCTG NGG 1 0.6522 Tier II
4 NC_000016.10 16 47464445 - PHKB NNNCCTCAAAATATTCCCTG NGG 2 0.6471 Tier II
5 NC_000011.10 11 16099657 + SOX6 NNNTCTAAAAGAATTCCCTG NGG 2 0.6 Tier II
6 NC_000006.12 6 169492371 - WDR27 NNNTCTCAAAGTATTATCTG NGG 2 0.4667 Tier II
7 NC_000012.12 12 95066885 - NR2C1 NNNTCTCAAAGTATTATCTG NGG 2 0.4667 Tier II
8 NC_000003.12 3 171157394 - TNIK NNNTCTCCAAGCATTCCCTG NGG 2 0.406 Tier II
9 NC_000006.12 6 89528542 + ANKRD6 NNNTCTCAAAGTAATCCATG NGG 2 0.3333 Tier II
10 NC_000001.11 1 233143447 - PCNX2 NNNCCTGAAAGTATTCCCTG NGG 2 0.3045 Tier II
11 NC_000016.10 16 12236706 - SNX29 NNNTCTCAAAGTGTTCACTG NGG 2 0.3043 Tier II
12 NC_000005.10 5 32590108 - SUB1 NNNGCTCTAAGTATTCCCTG NGG 2 0.2679 Tier II
13 NC_000004.12 4 155683866 + GUCY1A1 NNNTCTCAAAGTATTCCCTG NAG 1 0.2593 Tier II
14 NC_000018.10 18 13464602 - LDLRAD4 NNNCCTCAAATTATTCCCTG NGG 2 0.2489 Tier II
15 NC_000013.11 13 75642180 + LMO7 NNNTGTCAAAGTATTTCCTG NGG 2 0.1846 Tier II
16 NC_000008.11 8 11014606 + XKR6 NNNGCTCAAAGTCTTCCCTG NGG 2 0.1316 Tier II
17 NC_000004.12 4 174672407 + GLRA3 NNNTCTCCAAGTCTTCCCTG NGG 2 0.0902 Tier II
18 NC_000023.11 X 134404273 - PHF6 NNNTCTCAAAGTAATCCGTG NGG 2 0.0825 Tier II
19 NC_000002.12 2 27615627 - ZNF512 NNNTCTCAAAATATTCCCTG NGA 2 0.0694 Tier II
20 NC_000009.12 9 70309757 + SMC5 NNNTCTCAAAGTCTTCCCTG NAG 2 0.0546 Tier II
21 NC_000002.12 2 148054746 - MBD5 NNNTCTCAAAGTATTCACTG NGA 2 0.0324 Tier II
22 NC_000008.11 8 19675939 + CSGALNACT1 NNNTCTCAAAGTAATCCCTG NTG 2 0.0241 Tier II
23 NC_000006.12 6 85454237 - NT5E NNNACTCAAAGTATGCCCTG NGG 2 0.0238 Tier II
24 NC_000023.11 X 44237263 - EFHC2 NNNTCTCAAAGTATGCCCAG NGG 2 0.0143 Tier II
25 NC_000001.11 1 92125455 - PRKAR1AP1 NNNTCTCAAAGTGTTCCCTG NGG 1 0.6522 Tier III
26 NC_000003.12 3 171157394 - LOC105374216 NNNTCTCCAAGCATTCCCTG NGG 2 0.406 Tier III
27 NC_000003.12 3 144304515 - LOC105374140 NNNTCTCATAGTATACCCTG NGG 2 0.3474 Tier III
28 NC_000002.12 2 134721146 + LOC105373629 NNNTCTCAAAGTATCACCTG NGG 2 0.2727 Tier III
29 NC_000001.11 1 193724487 - LOC124904475 NNNTCTAAAAGTATTCCCAG NGG 2 0.2143 Tier III
30 NC_000007.14 7 56664933 - LOC102723684 NNNTCTCACAGTATCCCCTG NGG 2 0.1558 Tier III
31 NC_000007.14 7 1588891 + PSMG3-AS1 NNNCCTCAAAGTCTTCCCTG NGG 2 0.1362 Tier III
32 NC_000001.11 1 218919687 + LOC124904513 NNNTCTCAAAGTCATCCCTG NGG 2 0.1303 Tier III
33 NC_000002.12 2 148054746 - LOC105373673 NNNTCTCAAAGTATTCACTG NGA 2 0.0324 Tier III
34 NC_000005.10 5 181431511 + LOC124901156 NNNTCTCAAAGTATTCTCTG NGT 2 0.0075 Tier III
35 NC_000006.12 6 170707115 + LOC101929692 NNNTCTCAAAGTATTCTCTG NGT 2 0.0075 Tier III
36 NC_000008.11 8 101113 - LOC101927506 NNNTCTCAAAGTATTCTCTG NGT 2 0.0075 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000077.6 11 109653937 - Prkar1a NNNTCTCAAAGTATTCCCGA NGG 2 0.2344 Tier I
2 NC_000068.7 2 164497922 + Pigt NNNTCTCCAAGTACTCCCTG NGG 2 0.1224 Tier I
3 NC_000067.6 1 171226143 - Apoa2 NNNTCTCAAAGTATGCCCTG NGG 1 0.05 Tier I
4 NC_000073.6 7 82383968 - Adamtsl3 NNNTATCAAAGCATTCCCTG NGG 2 0.5414 Tier II
5 NC_000084.6 18 37452080 + Gm37013 NNNTCTCATTGTATTCCCTG NGG 2 0.5294 Tier II
6 NC_000084.6 18 37452080 + Gm38666 NNNTCTCATTGTATTCCCTG NGG 2 0.5294 Tier II
7 NC_000084.6 18 37452080 + Gm38667 NNNTCTCATTGTATTCCCTG NGG 2 0.5294 Tier II
8 NC_000069.6 3 154116213 - Slc44a5 NNNTCTCAAAGCATTCTCTG NGG 2 0.4421 Tier II
9 NC_000075.6 9 123828141 - Fyco1 NNNTCTCAAGGTATTCCTTG NGG 2 0.3571 Tier II
10 NC_000077.6 11 36035547 - Tenm2 NNNTCTCAAAGTATTCACTT NGG 2 0.3267 Tier II
11 NC_000084.6 18 61078411 - Pdgfrb NNNTCTCTAAGTGTTCCCTG NGG 2 0.2795 Tier II
12 NC_000077.6 11 30445898 - 4930505A04Rik NNNTCTCAAAGTATACTCTG NGG 2 0.2702 Tier II
13 NC_000080.6 14 10322953 - Fhit NNNTCTCAAAGCATTCCCTG NAG 2 0.2456 Tier II
14 NC_000082.6 16 18453418 + Txnrd2 NNNTCTCAACGTGTTCCCTG NGG 2 0.2174 Tier II
15 NC_000077.6 11 29736684 - Rtn4 NNNTCTTAAAGTATTCCCTG NAG 2 0.2106 Tier II
16 NC_000085.6 19 29367319 - Cd274 NNNTCTCATAGTATTTCCTG NGG 2 0.1846 Tier II
17 NC_000076.6 10 4961269 - Esr1 NNNTCTGAAAGTTTTCCCTG NGG 2 0.1412 Tier II
18 NC_000084.6 18 5006994 + Svil NNNTGTCAAAGTCTTCCCTG NGG 2 0.1263 Tier II
19 NC_000080.6 14 117427984 + Gpc6 NNNTCTCAAAGTATTTCCTG NAG 2 0.0798 Tier II
20 NC_000082.6 16 59426145 - Gabrr3 NNNTCTCAAAGAATTCCCTG NGC 2 0.0178 Tier II
21 NC_000069.6 3 132684815 + Tbck NNNTTTCAAAGTATTCCCTG NGT 2 0.0103 Tier II
22 NC_000075.6 9 82864426 + Gm38553 NNNTTTCAAAGTATTCACTG NGG 2 0.297 Tier III
23 NC_000083.6 17 16442048 + Gm41534 NNNTCTCAAATTATACCCTG NGG 2 0.2227 Tier III
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Other clones with same target sequence:

(none)