Construct: sgRNA BRDN0001147052

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AATGAAGAGTACAACCTCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
JAK2 (3717)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75727

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 5069087 + JAK2 NNNGAAGAGTACAACCTCAG NGG 0 1.0 Tier I
2 NC_000009.12 9 5069087 + INSL6 NNNGAAGAGTACAACCTCAG NGG 0 1.0 Tier II
3 NC_000019.10 19 45598836 - GPR4 NNNGAAGAGAAAAACCTCAG NGG 2 0.6122 Tier II
4 NC_000021.9 21 46421139 - PCNT NNNGCAGAGTACAACCTCAG NGG 1 0.5 Tier II
5 NC_000015.10 15 68354996 + ITGA11 NNNGAAGATTGCAACCTCAG NGG 2 0.3467 Tier II
6 NC_000009.12 9 16039636 - CCDC171 NNNGAAGAGGACAACCTTAG NGG 2 0.3214 Tier II
7 NC_000008.11 8 123930955 - FER1L6 NNNGAAGAGTAGAACCCCAG NGG 2 0.3137 Tier II
8 NC_000007.14 7 158701259 + NCAPG2 NNNGAAGAGCACAACCTAAG NGG 2 0.2872 Tier II
9 NC_000005.10 5 54206265 - ARL15 NNNGAAGAGTACAAACTCAG NAG 2 0.0576 Tier II
10 NC_000017.11 17 37119037 + ACACA NNNGAAGAGTACATTCTCAG NGG 2 0.0356 Tier II
11 NC_000002.12 2 15616324 - DDX1 NNNGAAGAGTACCATCTCAG NGG 2 0.014 Tier II
12 NC_000014.9 14 106769875 - IGH NNNGATGAGTACAACCTCTG NGG 2 0.3846 Tier III
13 NC_000014.9 14 106722871 - IGH NNNGACGAGTACAACCTCTG NGG 2 0.2448 Tier III
14 NC_000015.10 15 93705774 - LOC107983974 NNNGAAGAGCAGAACCTCAG NGG 2 0.237 Tier III
15 NC_000002.12 2 181278592 - LINC01934 NNNGAAGAGCACAACCTCCG NGG 2 0.1103 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 29287966 + Jak2 NNNGGAGAATACAACCTCAG NGG 2 0.4629 Tier I
2 NC_000076.6 10 112282307 + Kcnc2 NNNGAACAGTACAACATCAG NGG 2 0.6875 Tier II
3 NC_000075.6 9 79684109 + Col12a1 NNNGAAGAAAACAACCTCAG NGG 2 0.551 Tier II
4 NC_000080.6 14 35186955 - Grid1 NNNGAAGAAAACAACCTCAG NGG 2 0.551 Tier II
5 NC_000077.6 11 12434140 + Cobl NNNGAAGAGTATAACCTCAG NGG 1 0.5385 Tier II
6 NC_000084.6 18 75071646 - Dym NNNGAAGGGTACAACCCCAG NGG 2 0.5176 Tier II
7 NC_000081.6 15 74537928 + Adgrb1 NNNGAAGACAACAACCTCAG NGG 2 0.4615 Tier II
8 NC_000072.6 6 105702148 + Cntn4 NNNGAAGATTACAACCTCAT NGG 2 0.3733 Tier II
9 NC_000068.7 2 4886188 - Sephs1 NNNGTAGAGTACAACCTCAG NGG 1 0.3636 Tier II
10 NC_000075.6 9 7967017 + Yap1 NNNGGAGAGGACAACCTCAG NGG 2 0.36 Tier II
11 NC_000067.6 1 177017798 + Sdccag8 NNNGAGGAGTCCAACCTCAG NGG 2 0.2857 Tier II
12 NC_000078.6 12 119432225 + Macc1 NNNGAGGAGTCCAACCTCAG NGG 2 0.2857 Tier II
13 NC_000071.6 5 100140710 + Tmem150c NNNGAAGAGCAGAACCTCAG NGG 2 0.237 Tier II
14 NC_000077.6 11 98838023 - Rapgefl1 NNNGCAGCGTACAACCTCAG NGG 2 0.2143 Tier II
15 NC_000070.6 4 131994335 + Epb41 NNNGAAGAGTTCAACCTCTG NGG 2 0.1657 Tier II
16 NC_000083.6 17 73828021 - Ehd3 NNNGAAGAGGACAACCTCAG NAG 2 0.1296 Tier II
17 NC_000078.6 12 98255799 + Galc NNNGAAGAGTACATACTCAG NGG 2 0.1185 Tier II
18 NC_000082.6 16 94835452 - Kcnj6 NNNGAAGAGTAAAAGCTCAG NGG 2 0.0357 Tier II
19 NC_000085.6 19 44050240 - Erlin1 NNNGAAGAGTGCAAGCTCAG NGG 2 0.0325 Tier II
20 NC_000085.6 19 46359697 + Mfsd13a NNNGAAGAGGACAACCTCAG NTG 2 0.0195 Tier II
21 NC_000078.6 12 82076473 - Gm30799 NNNGCAGAGTACAACCTTAG NGG 2 0.3214 Tier III
22 NC_000084.6 18 26120204 - Gm33228 NNNGAATATTACAACCTCAG NGG 2 0.3048 Tier III
23 NC_000083.6 17 31868545 + Gm30509 NNNGAAGACTACAACCTGAG NGG 2 0.0718 Tier III
Download CSV

Other clones with same target sequence:

(none)