Construct: sgRNA BRDN0001147152
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCCACAAAAACTCGCTAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80228
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
66300268 |
+ |
DIS3L |
NNNCACAAAAACATGCTAAG |
NGG |
2 |
0.5077 |
Tier II |
| 2 |
NC_000011.10 |
11 |
89224522 |
+ |
TYR |
NNNCACAAAAACTTTCTAAG |
NGG |
2 |
0.1048 |
Tier II |
| 3 |
NC_000020.11 |
20 |
19622042 |
- |
SLC24A3 |
NNNCACAAAAACTCCCTAAG |
NAG |
2 |
0.0707 |
Tier II |
| 4 |
NC_000005.10 |
5 |
60678377 |
- |
DEPDC1B |
NNNCACAAAAACTCGTTAAG |
NGA |
2 |
0.0214 |
Tier II |
| 5 |
NC_000006.12 |
6 |
24991707 |
+ |
RIPOR2 |
NNNCACCAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA9 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHAC2 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHAC1 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA13 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA12 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA11 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA10 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA8 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA7 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA6 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA5 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA4 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA3 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA2 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA1 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 21 |
NC_000007.14 |
7 |
25741253 |
+ |
LINC03007 |
NNNCACAAAAATTCCCTAAG |
NGG |
2 |
0.1469 |
Tier III |
| 22 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHA@ |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier III |
| 23 |
NC_000005.10 |
5 |
141009964 |
- |
PCDHACT |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
23476431 |
- |
Masp1 |
NNNAACAAAAACTAGCTAAG |
NGG |
2 |
0.2947 |
Tier II |
| 2 |
NC_000075.6 |
9 |
45402200 |
- |
Fxyd2 |
NNNCACAAAAGCTCCCTAAG |
NGG |
2 |
0.1773 |
Tier II |
| 3 |
NC_000072.6 |
6 |
129853616 |
+ |
Klra17 |
NNNCACAAAGACTCGCTAAG |
NAG |
2 |
0.144 |
Tier II |
| 4 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha4 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 5 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha6 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha7 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha5 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha11 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 9 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha10 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha1 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha9 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha3 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha12 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha2 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdha8 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdhac1 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 17 |
NC_000084.6 |
18 |
37185283 |
- |
Pcdhac2 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 18 |
NC_000084.6 |
18 |
37185283 |
- |
Gm37013 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 19 |
NC_000084.6 |
18 |
37185283 |
- |
Gm38666 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000084.6 |
18 |
37185283 |
- |
Gm38667 |
NNNTACAAAAACTGGCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 21 |
NC_000084.6 |
18 |
26095655 |
- |
Gm33228 |
NNNCACAAATAATCGCTAAG |
NGG |
2 |
0.6303 |
Tier III |
| 22 |
NC_000084.6 |
18 |
32705199 |
+ |
4930455D15Rik |
NNNCACCAAAACTAGCTAAG |
NGG |
2 |
0.1531 |
Tier III |
Other clones with same target sequence:
(none)
