Construct: sgRNA BRDN0001147365

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GATAGCCATTTACTGCATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGFR2 (2263)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76183

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 121515257 - FGFR2 NNNAGCCATTTACTGCATAG NGG 0 1.0 Tier I
2 NC_000005.10 5 139127963 + SIL1 NNNAGACATTTCCTGCATAG NGG 2 0.2444 Tier I
3 NC_000005.10 5 22763918 - CDH12 NNNAGCCAATTACTGCATAG NGG 1 0.9286 Tier II
4 NC_000018.10 18 11826718 + GNAL NNNTGCCATTTACTGCAAAG NGG 2 0.4242 Tier II
5 NC_000019.10 19 54141054 - CNOT3 NNNAGCCTCTTACTGCATAG NGG 2 0.3956 Tier II
6 NC_000016.10 16 23280711 - SCNN1B NNNAGCCACTGACTGCATAG NGG 2 0.3692 Tier II
7 NC_000004.12 4 172784866 - GALNTL6 NNNAGGCATTTGCTGCATAG NGG 2 0.3611 Tier II
8 NC_000004.12 4 21029814 + KCNIP4 NNNAGCCTTTAACTGCATAG NGG 2 0.3214 Tier II
9 NC_000012.12 12 40814447 + CNTN1 NNNAGCCATTTACTGAATAG NAG 2 0.2593 Tier II
10 NC_000001.11 1 45196942 + ZSWIM5 NNNAGCCATTTACTGACTAG NGG 2 0.1765 Tier II
11 NC_000004.12 4 93219758 + GRID2 NNNATCCATTTACTGCTTAG NGG 2 0.04 Tier II
12 NC_000006.12 6 110158983 + WASF1 NNNAGCCATTTACTGCATTG NGA 2 0.0374 Tier II
13 NC_000005.10 5 141380667 + PCDHGB3 NNNAGCCATTTACTCCATAG NGT 2 0.0044 Tier II
14 NC_000005.10 5 141380667 + PCDHGB2 NNNAGCCATTTACTCCATAG NGT 2 0.0044 Tier II
15 NC_000005.10 5 141380667 + PCDHGB1 NNNAGCCATTTACTCCATAG NGT 2 0.0044 Tier II
16 NC_000005.10 5 141380667 + PCDHGA6 NNNAGCCATTTACTCCATAG NGT 2 0.0044 Tier II
17 NC_000005.10 5 141380667 + PCDHGA5 NNNAGCCATTTACTCCATAG NGT 2 0.0044 Tier II
18 NC_000005.10 5 141380667 + PCDHGA4 NNNAGCCATTTACTCCATAG NGT 2 0.0044 Tier II
19 NC_000005.10 5 141380667 + PCDHGA3 NNNAGCCATTTACTCCATAG NGT 2 0.0044 Tier II
20 NC_000005.10 5 141380667 + PCDHGA2 NNNAGCCATTTACTCCATAG NGT 2 0.0044 Tier II
21 NC_000005.10 5 141380667 + PCDHGA1 NNNAGCCATTTACTCCATAG NGT 2 0.0044 Tier II
22 NC_000022.11 22 35132057 + LINC01399 NNNAGCCACTTACTGCAAAG NGG 2 0.6154 Tier III
23 NC_000016.10 16 17932956 + RPL7P47 NNNAGCCATATACTGCATGG NGG 2 0.3214 Tier III
24 NC_000016.10 16 17932956 + LOC107984893 NNNAGCCATATACTGCATGG NGG 2 0.3214 Tier III
25 NC_000013.11 13 41552209 - LOC105370174 NNNAGCCATTCACTGCACAG NGG 2 0.2857 Tier III
26 NC_000013.11 13 41552209 - LOC105370175 NNNAGCCATTCACTGCACAG NGG 2 0.2857 Tier III
27 NC_000004.12 4 96732136 - LINC02267 NNNAGCCATTTACTGCATAC NAG 2 0.1111 Tier III
28 NC_000005.10 5 141380667 + PCDHG@ NNNAGCCATTTACTCCATAG NGT 2 0.0044 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 130196312 - Fgfr2 NNNAGCTATTTACTGCATAG NGG 1 0.8125 Tier I
2 NC_000067.6 1 32358026 + Khdrbs2 NNNGGCCATTTACTGAATAG NGG 2 0.625 Tier II
3 NC_000073.6 7 55260872 - Luzp2 NNNAGCAATTTGCTGCATAG NGG 2 0.5417 Tier II
4 NC_000069.6 3 28301428 + Tnik NNNAGCCATATACAGCATAG NGG 2 0.5306 Tier II
5 NC_000077.6 11 58875070 - 2810021J22Rik NNNTCCCATTTACTGCATAG NGG 2 0.5 Tier II
6 NC_000075.6 9 97611621 + Clstn2 NNNAGCCATTTGCAGCATAG NGG 2 0.4471 Tier II
7 NC_000067.6 1 88409542 - Spp2 NNNAGCCATTTACTGCAATG NGG 2 0.359 Tier II
8 NC_000077.6 11 33452421 - Ranbp17 NNNAGCTATTTACTGCACAG NGG 2 0.3482 Tier II
9 NC_000076.6 10 77774119 - Tspear NNNAGCCATTAACTGCACAG NGG 2 0.3214 Tier II
10 NC_000067.6 1 8930194 + Sntg1 NNNAGCCAGGTACTGCATAG NGG 2 0.3095 Tier II
11 NC_000068.7 2 65141448 - Cobll1 NNNAGCCCTTTACTGCATTG NGG 2 0.2308 Tier II
12 NC_000084.6 18 33946336 - Epb41l4a NNNAGGCATTTACTGCACAG NGG 2 0.2143 Tier II
13 NC_000080.6 14 121663447 + Dock9 NNNAGCCATCTACTGCATGG NGG 2 0.2 Tier II
14 NC_000078.6 12 44281856 + Pnpla8 NNNAGCCATTTACTAGATAG NGG 2 0.1448 Tier II
15 NC_000078.6 12 108373464 + Eml1 NNNTGCCATTTACTGCCTAG NGG 2 0.1123 Tier II
16 NC_000076.6 10 53629270 + Mcm9 NNNAGCCATTTACTGTCTAG NGG 2 0.0543 Tier II
17 NC_000071.6 5 64381164 + Gm43837 NNNAGCCATTTACTGCTAAG NGG 2 0.0889 Tier III
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Other clones with same target sequence:

(none)