Construct: sgRNA BRDN0001147643

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGTGGAGAGTACATCAGCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP3K12 (7786)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76761

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 53484301 + MAP3K12 NNNGGAGAGTACATCAGCTG NGG 0 1.0 Tier I
2 NC_000006.12 6 143176316 + AIG1 NNNGAAGAGTGCATCAGCTG NGG 2 0.5633 Tier II
3 NC_000020.11 20 9328141 + PLCB4 NNNGGAGAGTACATCAAATG NGG 2 0.5026 Tier II
4 NC_000007.14 7 110737714 - IMMP2L NNNGAAGAGCACATCAGCTG NGG 2 0.4622 Tier II
5 NC_000015.10 15 54198357 - UNC13C NNNCAAGAGTACATCAGCTG NGG 2 0.4588 Tier II
6 NC_000005.10 5 177261317 - NSD1 NNNGGAGAGTAGATCAACTG NGG 2 0.4148 Tier II
7 NC_000005.10 5 149823568 - PPARGC1B NNNGGAGAGTTAATCAGCTG NGG 2 0.2198 Tier II
8 NC_000001.11 1 161915095 - ATF6 NNNGGTGAGTTCATCAGCTG NGG 2 0.2198 Tier II
9 NC_000009.12 9 124963035 - SCAI NNNGGAGGGTACATCACCTG NGG 2 0.1725 Tier II
10 NC_000013.11 13 19730740 + PSPC1 NNNGGCGAGTACATCACCTG NGG 2 0.107 Tier II
11 NC_000009.12 9 129069747 + MIGA2 NNNGGAAAGTACATGAGCTG NGG 2 0.05 Tier II
12 NC_000013.11 13 49585239 + RCBTB1 NNNGGAGAGCACATTAGCTG NGG 2 0.0356 Tier II
13 NC_000001.11 1 226640391 + ITPKB NNNGGAGAGTACTTCAGCTG NGT 2 0.0048 Tier II
14 NC_000009.12 9 96858890 + ZNF782 NNNGGAGAGCACAGCAGCTG NGG 2 0.0 Tier II
15 NC_000013.11 13 99056680 + DOCK9 NNNGGAGAGTACACCTGCTG NGG 2 0.0 Tier II
16 NC_000010.11 10 102411909 + PSD NNNTGAGAGTACAGCAGCTG NGG 2 0.0 Tier II
17 NC_000003.12 3 82204978 - LINC02008 NNNGGAGAGGAAATCAGCTG NGG 2 0.3571 Tier III
18 NC_000013.11 13 58751233 + DNAJA1P1 NNNAGAGAGTTCATCAGCTG NGG 2 0.2769 Tier III
19 NC_000013.11 13 58751233 + LOC105370220 NNNAGAGAGTTCATCAGCTG NGG 2 0.2769 Tier III
20 NC_000008.11 8 116110828 + LINC00536 NNNGGAGAGGAGATCAGCTG NGG 2 0.2222 Tier III
21 NC_000011.10 11 59547528 + LOC102723575 NNNGGAGAGTAAATCACCTG NGG 2 0.1681 Tier III
22 NC_000012.12 12 105567506 - LOC124903006 NNNGGAGAGTGCATCAGCTG NTG 2 0.0253 Tier III
23 NC_000003.12 3 30385229 - LOC101927995 NNNGGAGAGTACAGCAACTG NGG 2 0.0 Tier III
24 NC_000003.12 3 66922301 + LOC105377144 NNNGGAGAGTGCAGCAGCTG NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000067.6 1 37625639 - Cracdl NNNGGAGAGTACATCAGAGG NGG 2 0.1346 Tier I
2 NC_000070.6 4 131886836 + Srsf4 NNNGAGGAGTACATCAGCTG NGG 2 0.619 Tier II
3 NC_000071.6 5 105577058 + Lrrc8c NNNGGAGAGAATATCAGCTG NGG 2 0.4615 Tier II
4 NC_000067.6 1 85613359 + Sp140 NNNGGTGATTACATCAGCTG NGG 2 0.381 Tier II
5 NT_166280.1 1 128666 + LOC101055672 NNNGGTGATTACATCAGCTG NGG 2 0.381 Tier II
6 NC_000070.6 4 118119843 - St3gal3 NNNAGAGAGTCCATCAGCTG NGG 2 0.36 Tier II
7 NC_000067.6 1 85095762 - A530032D15Rik NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier II
8 NC_000067.6 1 85162510 + Gm38510 NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier II
9 NC_000067.6 1 85255982 - C130026I21Rik NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier II
10 NC_000067.6 1 85255982 - Gm38510 NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier II
11 NC_000067.6 1 85489857 + Gm7592 NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier II
12 NT_162750.1 1 118760 + Csprs NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier II
13 NT_166280.1 1 14433 + LOC100041708 NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier II
14 NT_166281.1 1 180815 + LOC100041057 NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier II
15 NT_166282.1 1 90458 - LOC100041903 NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier II
16 NC_000072.6 6 106664735 - Cntn4 NNNGGAGAGTAGATCAGCTC NGG 2 0.1905 Tier II
17 NC_000068.7 2 72001143 - Rapgef4 NNNGGAGAGTGCATCAGCAG NGG 2 0.1857 Tier II
18 NC_000067.6 1 131271462 + Ikbke NNNGGAGAGTTTATCAGCTG NGG 2 0.1657 Tier II
19 NC_000081.6 15 94723093 - Tmem117 NNNTGAGTGTACATCAGCTG NGG 2 0.1558 Tier II
20 NC_000071.6 5 31943350 + Babam2 NNNGGAGACTACATCAGCAG NGG 2 0.1538 Tier II
21 NC_000072.6 6 52722705 - Tax1bp1 NNNGGAGAGTACTTCAGCTG NAG 2 0.0778 Tier II
22 NC_000078.6 12 3783441 - Dtnb NNNGAAGAGTACATTAGCTG NGG 2 0.0578 Tier II
23 NC_000077.6 11 37222894 + Tenm2 NNNGGAGAGTACCTCAGGTG NGG 2 0.0281 Tier II
24 NC_000070.6 4 106431971 - Usp24 NNNGGAGAGTACAGCAGCTG NTG 2 0.0 Tier II
25 NC_000072.6 6 30615707 + Cpa5 NNNGGAGAGTACGGCAGCTG NGG 2 0.0 Tier II
26 NC_000077.6 11 57314150 + Gria1 NNNGGAGAGTGCATCTGCTG NGG 2 0.0 Tier II
27 NT_162750.1 1 51367 + LOC115489307 NNNGGTGATTACATCAGCTG NGG 2 0.381 Tier III
28 NT_162750.1 1 51367 + LOC115489308 NNNGGTGATTACATCAGCTG NGG 2 0.381 Tier III
29 NC_000067.6 1 85489857 + Gm2666 NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier III
30 NT_162750.1 1 118760 + LOC105242429 NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier III
31 NT_166456.1 23720 + LOC673191 NNNGGCGATTACATCAGCTG NGG 2 0.2424 Tier III
32 NC_000080.6 14 59070094 - Gm41162 NNNGGAGAGTATATCAGCTC NGG 2 0.2308 Tier III
33 NC_000070.6 4 135288858 - Gm30767 NNNGGAGAGTACAGCAGCTG NGG 1 0.0 Tier III
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Other clones with same target sequence:

(none)