Construct: sgRNA BRDN0001148231
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AACAGATGTCAGGTTATAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAOK3 (51347)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76737
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
118181545 |
- |
TAOK3 |
NNNAGATGTCAGGTTATAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
65665380 |
- |
TRAPPC13 |
NNNAAATATCAGGTTATAAG |
NGG |
2 |
0.8667 |
Tier II |
| 3 |
NC_000020.11 |
20 |
19813571 |
+ |
RIN2 |
NNNACATGTCAGGTTATAAT |
NGG |
2 |
0.55 |
Tier II |
| 4 |
NC_000007.14 |
7 |
90260761 |
- |
CFAP69 |
NNNAGATGTCAGGTTAAAAT |
NGG |
2 |
0.3733 |
Tier II |
| 5 |
NC_000001.11 |
1 |
61232448 |
+ |
NFIA |
NNNAGATGACATGTTATAAG |
NGG |
2 |
0.3571 |
Tier II |
| 6 |
NC_000001.11 |
1 |
85035112 |
- |
MCOLN3 |
NNNAGATGTCAAGTTATAGG |
NGG |
2 |
0.35 |
Tier II |
| 7 |
NC_000009.12 |
9 |
19774602 |
+ |
SLC24A2 |
NNNAGATGACAGTTTATAAG |
NGG |
2 |
0.2786 |
Tier II |
| 8 |
NC_000015.10 |
15 |
72022042 |
- |
MYO9A |
NNNAGATGTCAGGTAATAAC |
NGG |
2 |
0.2481 |
Tier II |
| 9 |
NC_000010.11 |
10 |
13793816 |
+ |
FRMD4A |
NNNAGATGTCAGGTTATAAA |
NAG |
2 |
0.2431 |
Tier II |
| 10 |
NC_000010.11 |
10 |
52000631 |
- |
PRKG1 |
NNNAGATGTCAAGTTATAAG |
NAG |
2 |
0.242 |
Tier II |
| 11 |
NC_000005.10 |
5 |
33625445 |
- |
ADAMTS12 |
NNNAGATGTCAGCTTATTAG |
NGG |
2 |
0.2105 |
Tier II |
| 12 |
NC_000017.11 |
17 |
82171663 |
+ |
CCDC57 |
NNNAGATTTCAGTTTATAAG |
NGG |
2 |
0.1875 |
Tier II |
| 13 |
NC_000012.12 |
12 |
88102545 |
+ |
CEP290 |
NNNAGATGTCTGGTTAAAAG |
NGG |
2 |
0.1641 |
Tier II |
| 14 |
NC_000023.11 |
X |
38660497 |
- |
TSPAN7 |
NNNAGATGTTAGGTTTTAAG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000013.11 |
13 |
71595716 |
+ |
DACH1 |
NNNATATGTCAGGTTCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000009.12 |
9 |
138140609 |
+ |
IL9RP1 |
NNNACATGTCAGGTTACAAG |
NGG |
2 |
0.5546 |
Tier III |
| 17 |
NC_000010.11 |
10 |
83700 |
- |
IL9RP2 |
NNNACATGTCAGGTTACAAG |
NGG |
2 |
0.5546 |
Tier III |
| 18 |
NC_000016.10 |
16 |
32724 |
- |
IL9RP3 |
NNNACATGTCAGGTTACAAG |
NGG |
2 |
0.5546 |
Tier III |
| 19 |
NC_000018.10 |
18 |
83533 |
- |
IL9RP4 |
NNNACATGTCAGGTTACAAG |
NGG |
2 |
0.5546 |
Tier III |
| 20 |
NC_000016.10 |
16 |
55446329 |
- |
MMP2-AS1 |
NNNAGATGTCACGTTATAAA |
NGG |
2 |
0.4963 |
Tier III |
| 21 |
NC_000015.10 |
15 |
72022042 |
- |
LOC107984714 |
NNNAGATGTCAGGTAATAAC |
NGG |
2 |
0.2481 |
Tier III |
| 22 |
NC_000006.12 |
6 |
31298656 |
- |
LINC02571 |
NNNAGATGTCAGGTTCTCAG |
NGG |
2 |
0.0 |
Tier III |
| 23 |
NC_000006.12 |
6 |
31298656 |
- |
LOC112267902 |
NNNAGATGTCAGGTTCTCAG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
117250924 |
+ |
Taok3 |
NNNAGATGTCAGGTTATAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000077.6 |
11 |
77553847 |
- |
Taok1 |
NNNAGATGTCTGGTTATAAG |
NGG |
1 |
0.3077 |
Tier I |
| 3 |
NC_000074.6 |
8 |
33257235 |
+ |
Wrn |
NNNAGATGGCAGGTTCTAAG |
NGG |
2 |
0.0 |
Tier I |
| 4 |
NC_000071.6 |
5 |
148892572 |
+ |
Katnal1 |
NNNAGATGTCAGGGTAGAAG |
NGG |
2 |
0.0 |
Tier I |
| 5 |
NC_000075.6 |
9 |
118875070 |
- |
Itga9 |
NNNAGATGTTAAGTTATAAG |
NGG |
2 |
0.8784 |
Tier II |
| 6 |
NC_000069.6 |
3 |
63948383 |
+ |
Slc33a1 |
NNNAGATGCCAAGTTATAAG |
NGG |
2 |
0.8615 |
Tier II |
| 7 |
NC_000077.6 |
11 |
54064000 |
+ |
Pdlim4 |
NNNAGATGTAAGGTTATATG |
NGG |
2 |
0.4667 |
Tier II |
| 8 |
NC_000070.6 |
4 |
97916118 |
+ |
Nfia |
NNNAGATGACATGTTATAAG |
NGG |
2 |
0.3571 |
Tier II |
| 9 |
NC_000084.6 |
18 |
37291029 |
- |
Gm37013 |
NNNAAATGTCAGGTTATGAG |
NGG |
2 |
0.3467 |
Tier II |
| 10 |
NC_000084.6 |
18 |
37291029 |
- |
Gm38666 |
NNNAAATGTCAGGTTATGAG |
NGG |
2 |
0.3467 |
Tier II |
| 11 |
NC_000084.6 |
18 |
37291029 |
- |
Gm38667 |
NNNAAATGTCAGGTTATGAG |
NGG |
2 |
0.3467 |
Tier II |
| 12 |
NC_000069.6 |
3 |
155030009 |
- |
Tnni3k |
NNNAGATGTCTGGATATAAG |
NGG |
2 |
0.1905 |
Tier II |
| 13 |
NC_000084.6 |
18 |
33118453 |
- |
Camk4 |
NNNAGATGCCAGGTTGTAAG |
NGG |
2 |
0.1775 |
Tier II |
| 14 |
NC_000078.6 |
12 |
31305043 |
- |
Lamb1 |
NNNAGAGGTCAGGTTATAAG |
NAG |
2 |
0.1525 |
Tier II |
| 15 |
NC_000081.6 |
15 |
47626473 |
+ |
Csmd3 |
NNNATATGTCATGTTATAAG |
NGG |
2 |
0.1154 |
Tier II |
| 16 |
NC_000080.6 |
14 |
10621500 |
- |
Fhit |
NNNGGATGTCAGGTTAGAAG |
NGG |
2 |
0.0735 |
Tier II |
| 17 |
NC_000082.6 |
16 |
9783079 |
+ |
Grin2a |
NNNAGATGTCAGGTTATAAG |
NGC |
1 |
0.0222 |
Tier II |
| 18 |
NC_000067.6 |
1 |
80677435 |
+ |
Dock10 |
NNNAGATGTCAGGTTGTAAG |
NGC |
2 |
0.0043 |
Tier II |
| 19 |
NC_000083.6 |
17 |
84552805 |
+ |
Plekhh2 |
NNNAGATGTCAGGATCTAAG |
NGG |
2 |
0.0 |
Tier II |
| 20 |
NC_000069.6 |
3 |
119293052 |
+ |
Dpyd |
NNNAGATGTCAGGTTTTAAG |
NGC |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
