Construct: sgRNA BRDN0001148630

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGCACAGGAACACCCATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEK (7010)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75723

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 27173316 - TEK NNNCACAGGAACACCCATAG NGG 0 1.0 Tier I
2 NC_000016.10 16 82660417 - CDH13 NNNCACAGGAACGCCCATAG NGG 1 0.6522 Tier II
3 NC_000017.11 17 28897310 + FLOT2 NNNCACAGGAAAACCCACAG NGG 2 0.3061 Tier II
4 NC_000018.10 18 45458399 + SLC14A2 NNNCACAGGAAAACCCAGAG NGG 2 0.2381 Tier II
5 NC_000007.14 7 82036072 - CACNA2D1 NNNCACAGGAACACACATAG NGG 1 0.2222 Tier II
6 NC_000002.12 2 45567964 + SRBD1 NNNCACAGGAAGACCCATGG NGG 2 0.1667 Tier II
7 NC_000007.14 7 23188089 - NUP42 NNNCCCAGGAACACCCAGAG NGG 2 0.1667 Tier II
8 NC_000012.12 12 121755954 + TMEM120B NNNCCCAGGAACACCCAGAG NGG 2 0.1667 Tier II
9 NC_000006.12 6 75309004 + FILIP1 NNNCACAGGAACACCCACAG NAG 2 0.1111 Tier II
10 NC_000014.9 14 101878843 + PPP2R5C NNNCACAGGAACACCCACAG NAG 2 0.1111 Tier II
11 NC_000010.11 10 123838901 - CPXM2 NNNCACAGGAACACCCCTGG NGG 2 0.0662 Tier II
12 NC_000002.12 2 23733932 + ATAD2B NNNCACAGAAACACCCATAG NTG 2 0.025 Tier II
13 NC_000002.12 2 27103237 - CGREF1 NNNCACAGGAACAGCCACAG NGG 2 0.0 Tier II
14 NC_000018.10 18 45458399 + SLC14A2-AS1 NNNCACAGGAAAACCCAGAG NGG 2 0.2381 Tier III
15 NC_000004.12 4 187656495 + LINC02492 NNNCACAGGAACACACATAA NGG 2 0.2083 Tier III
16 NC_000004.12 4 100191422 + DDIT4L-AS1 NNNCACAGGAACACACAGAG NGG 2 0.0741 Tier III
17 NC_000010.11 10 64649663 + LOC124902439 NNNCACAGGAACACCCTTAG NGA 2 0.0093 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000084.6 18 36994517 - Pcdha8 NNNCACAGTAACACCCACAG NGG 2 0.2286 Tier I
2 NC_000070.6 4 94804907 - Tek NNNCACAGGAACACCCGTAA NGG 2 0.1654 Tier I
3 NC_000070.6 4 118484123 + Tie1 NNNCACAGGAACAGCCATAG NGA 2 0.0 Tier I
4 NC_000070.6 4 85736014 + Adamtsl1 NNNCCCAGGAACACCAATAG NGG 2 0.5 Tier II
5 NC_000075.6 9 73768462 + Unc13c NNNCACAAGAAGACCCATAG NGG 2 0.4444 Tier II
6 NC_000075.6 9 50809256 + Alg9 NNNCACAGGAACACCCAGAA NGG 2 0.3125 Tier II
7 NC_000072.6 6 21390842 + Kcnd2 NNNCACAGGTACACCCATAG NAG 2 0.2288 Tier II
8 NC_000084.6 18 36994517 - Pcdha4 NNNCACAGTAACACCCACAG NGG 2 0.2286 Tier II
9 NC_000084.6 18 36994517 - Pcdha6 NNNCACAGTAACACCCACAG NGG 2 0.2286 Tier II
10 NC_000084.6 18 36994517 - Pcdha7 NNNCACAGTAACACCCACAG NGG 2 0.2286 Tier II
11 NC_000084.6 18 36994517 - Pcdha5 NNNCACAGTAACACCCACAG NGG 2 0.2286 Tier II
12 NC_000084.6 18 36994517 - Pcdha1 NNNCACAGTAACACCCACAG NGG 2 0.2286 Tier II
13 NC_000084.6 18 36994517 - Pcdha3 NNNCACAGTAACACCCACAG NGG 2 0.2286 Tier II
14 NC_000084.6 18 36994517 - Pcdha2 NNNCACAGTAACACCCACAG NGG 2 0.2286 Tier II
15 NC_000084.6 18 36994517 - Gm37013 NNNCACAGTAACACCCACAG NGG 2 0.2286 Tier II
16 NC_000084.6 18 36994517 - Gm38666 NNNCACAGTAACACCCACAG NGG 2 0.2286 Tier II
17 NC_000084.6 18 36994517 - Gm38667 NNNCACAGTAACACCCACAG NGG 2 0.2286 Tier II
18 NC_000071.6 5 120557509 + Tpcn1 NNNCCCAGGAAGACCCATAG NGG 2 0.2222 Tier II
19 NC_000076.6 10 10878419 - Grm1 NNNCACAGGTACACACATAG NGG 2 0.1961 Tier II
20 NC_000076.6 10 20473286 - Pde7b NNNCACAGCAACAACCATAG NGG 2 0.1885 Tier II
21 NC_000077.6 11 72408750 - Smtnl2 NNNCACAGGAACACCCACAC NGG 2 0.1837 Tier II
22 NC_000085.6 19 48354856 + Sorcs3 NNNCAGAGGAACACACATAG NGG 2 0.1111 Tier II
23 NC_000078.6 12 98824179 + Eml5 NNNCACAGGAACACACATAG NAG 2 0.0576 Tier II
24 NC_000071.6 5 12406785 - Sema3d NNNCACAGTAACACCCATAG NGA 2 0.037 Tier II
25 NC_000080.6 14 75192452 - Lcp1 NNNCACAGGAACACCCTTAG NAG 2 0.0346 Tier II
26 NC_000071.6 5 27275426 - Dpp6 NNNCACAGGAACACACATAG NGA 2 0.0154 Tier II
27 NC_000080.6 14 59470672 + Cab39l NNNGACAGGAACACCCATAG NGC 2 0.0111 Tier II
28 NC_000072.6 6 145784011 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
29 NC_000072.6 6 145784160 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
30 NC_000072.6 6 145784293 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
31 NC_000072.6 6 145784459 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
32 NC_000072.6 6 145784558 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
33 NC_000072.6 6 145784789 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
34 NC_000072.6 6 145784922 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
35 NC_000072.6 6 145785088 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
36 NC_000072.6 6 145785204 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
37 NC_000072.6 6 145785370 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
38 NC_000072.6 6 145785519 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
39 NC_000072.6 6 145785618 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
40 NC_000072.6 6 145785783 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
41 NC_000072.6 6 145785932 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
42 NC_000072.6 6 145786116 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
43 NC_000072.6 6 145786433 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
44 NC_000072.6 6 145786648 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
45 NC_000072.6 6 145786764 + Rassf8 NNNCACAGGAACACCCACAG NGC 2 0.0095 Tier II
46 NC_000077.6 11 5250984 - Kremen1 NNNCACAGCAACACCCATAG NGT 2 0.0087 Tier II
47 NC_000078.6 12 81052100 + Smoc1 NNNCACAGGAACACGGATAG NGG 2 0.0077 Tier II
48 NC_000080.6 14 56084297 - Mcpt8 NNNCACAGGAACACCCGTAG NTG 2 0.0069 Tier II
49 NC_000079.6 13 102354326 - Gm36703 NNNCACAGGACCACCAATAG NGG 2 0.4 Tier III
50 NC_000074.6 8 39749859 - Gm2033 NNNCACAGGAACACCAATGG NGG 2 0.375 Tier III
51 NC_000073.6 7 61138418 - A230006K03Rik NNNCACAGGATCACCAATAG NGG 2 0.3077 Tier III
52 NC_000067.6 1 161206347 - Gm6185 NNNCACAGGAAGACCCACAG NGG 2 0.1905 Tier III
53 NC_000084.6 18 83011340 - Gm41795 NNNCACAGGAACCCCCAAAG NGG 2 0.1404 Tier III
54 NC_000075.6 9 83885209 - Gm39387 NNNCACAGGAACTCACATAG NGG 2 0.0667 Tier III
55 NC_000078.6 12 98824179 + Gm35463 NNNCACAGGAACACACATAG NAG 2 0.0576 Tier III
56 NC_000071.6 5 28084979 + Gm35223 NNNCACAGGAACACCCCTCG NGG 2 0.0365 Tier III
57 NC_000075.6 9 8683691 - Gm32014 NNNCACAGGAACACACATAG NTG 2 0.0087 Tier III
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Other clones with same target sequence:

(none)