Construct: sgRNA BRDN0001149447
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGCAGAAGTTGCTGTCCTG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000015.10 | 15 | 42675729 | + | STARD9 | NNNCAGAGGCTGCTGTCCTG | NGG | 2 | 0.3911 | Tier I |
2 | NC_000005.10 | 5 | 56882666 | + | MAP3K1 | NNNCAGAAGTTGCTGTCCTG | NCT | 2 | 0.0 | Tier I |
3 | NC_000005.10 | 5 | 1336619 | + | CLPTM1L | NNNCAGAACTTGCTGTCCTG | NGG | 1 | 0.5385 | Tier II |
4 | NC_000019.10 | 19 | 47665391 | + | BICRA | NNNCAAAAGTTCCTGTCCTG | NGG | 2 | 0.5294 | Tier II |
5 | NC_000002.12 | 2 | 134447997 | - | MGAT5 | NNNCAGAAGGTGCTGTCCTG | NGG | 1 | 0.5 | Tier II |
6 | NC_000001.11 | 1 | 183102253 | + | LAMC1 | NNNCAGAAGTTGCTGTTCTG | NGG | 1 | 0.4667 | Tier II |
7 | NC_000020.11 | 20 | 9563652 | + | PAK5 | NNNCAGAAATTGCTGCCCTG | NGG | 2 | 0.4286 | Tier II |
8 | NC_000002.12 | 2 | 115360263 | + | DPP10 | NNNCAGAAGTTGCTGTCATT | NGG | 2 | 0.3769 | Tier II |
9 | NC_000010.11 | 10 | 116101135 | - | GFRA1 | NNNCAGAAGGAGCTGTCCTG | NGG | 2 | 0.375 | Tier II |
10 | NC_000016.10 | 16 | 81625287 | + | CMIP | NNNCAGAAGCTGCAGTCCTG | NGG | 2 | 0.3302 | Tier II |
11 | NC_000010.11 | 10 | 101580158 | - | POLL | NNNCAGAAGTTCCAGTCCTG | NGG | 2 | 0.3277 | Tier II |
12 | NC_000001.11 | 1 | 181556308 | - | CACNA1E | NNNGAGAAATTGCTGTCCTG | NGG | 2 | 0.3214 | Tier II |
13 | NC_000022.11 | 22 | 38326025 | + | TPTEP2-CSNK1E | NNNCTGAAGATGCTGTCCTG | NGG | 2 | 0.3117 | Tier II |
14 | NC_000007.14 | 7 | 158217466 | - | PTPRN2 | NNNTTGAAGTTGCTGTCCTG | NGG | 2 | 0.2909 | Tier II |
15 | NC_000010.11 | 10 | 51331023 | + | PRKG1 | NNNCAGCAGTTGCAGTCCTG | NGG | 2 | 0.2708 | Tier II |
16 | NC_000001.11 | 1 | 178767352 | + | RALGPS2 | NNNCTGAAGTTGCTGTCCTT | NGG | 2 | 0.2545 | Tier II |
17 | NC_000013.11 | 13 | 99311352 | + | UBAC2 | NNNGAGAAGGTGCTGTCCTG | NGG | 2 | 0.25 | Tier II |
18 | NC_000010.11 | 10 | 105219587 | - | SORCS3 | NNNCTGAAATTGCTGTCCTG | NGG | 2 | 0.2338 | Tier II |
19 | NC_000001.11 | 1 | 11149447 | - | MTOR | NNNCAGAAGCTTCTGTCCTG | NGG | 2 | 0.2051 | Tier II |
20 | NC_000001.11 | 1 | 243783422 | - | AKT3 | NNNCAGAAGCTTCTGTCCTG | NGG | 2 | 0.2051 | Tier II |
21 | NC_000006.12 | 6 | 34391130 | - | NUDT3 | NNNCAGGAGTTGCTGTCCAG | NGG | 2 | 0.2017 | Tier II |
22 | NC_000006.12 | 6 | 34391130 | - | RPS10-NUDT3 | NNNCAGGAGTTGCTGTCCAG | NGG | 2 | 0.2017 | Tier II |
23 | NC_000022.11 | 22 | 35417972 | + | MCM5 | NNNCAGATGTTGCTGTTCTG | NGG | 2 | 0.2 | Tier II |
24 | NC_000023.11 | X | 85055345 | + | APOOL | NNNCAGCAGTGGCTGTCCTG | NGG | 2 | 0.175 | Tier II |
25 | NC_000002.12 | 2 | 75650905 | - | MRPL19 | NNNCAGAAGGTGCTGTCCAG | NGG | 2 | 0.1429 | Tier II |
26 | NC_000010.11 | 10 | 101526493 | + | BTRC | NNNCAGAAGTTGGTATCCTG | NGG | 2 | 0.1283 | Tier II |
27 | NC_000006.12 | 6 | 146212043 | + | GRM1 | NNNCAGAAGTTGCTGTCCAC | NGG | 2 | 0.1224 | Tier II |
28 | NC_000015.10 | 15 | 71705537 | + | THSD4 | NNNCAGATGTTGCTGTCCTG | NAG | 2 | 0.1111 | Tier II |
29 | NC_000010.11 | 10 | 126108878 | - | ADAM12 | NNNCAGAAGTTGCTGTCTTG | NGA | 2 | 0.0446 | Tier II |
30 | NC_000012.12 | 12 | 51826688 | - | FIGNL2 | NNNCAGAAGTTGCTGACCTG | NTG | 2 | 0.0354 | Tier II |
31 | NC_000001.11 | 1 | 213603272 | + | RPS6KC1 | NNNCAGAGGTTGCTGTCCTG | NTG | 2 | 0.0286 | Tier II |
32 | NC_000020.11 | 20 | 16323820 | + | KIF16B | NNNCAGAAGCTGCTGTCCTG | NTG | 2 | 0.0208 | Tier II |
33 | NC_000003.12 | 3 | 70099939 | + | SAMMSON | NNNCAGAAGTAACTGTCCTG | NGG | 2 | 0.7 | Tier III |
34 | NC_000003.12 | 3 | 110658391 | + | LOC105374037 | NNNCAGAAGCTGCTGACCTG | NGG | 2 | 0.4848 | Tier III |
35 | NC_000010.11 | 10 | 5275276 | - | AKR1C7P | NNNAAGAAGCTGCTGTCCTG | NGG | 2 | 0.4491 | Tier III |
36 | NC_000020.11 | 20 | 9563652 | + | LOC105372523 | NNNCAGAAATTGCTGCCCTG | NGG | 2 | 0.4286 | Tier III |
37 | NC_000018.10 | 18 | 75439548 | + | LOC124900405 | NNNCAGAAGTGGCTGTCCTG | NGG | 1 | 0.4 | Tier III |
38 | NC_000011.10 | 11 | 30296314 | - | ARL14EP-DT | NNNTTGAAGTTGCTGTCCTG | NGG | 2 | 0.2909 | Tier III |
39 | NC_000011.10 | 11 | 34771917 | + | LOC102723568 | NNNCTGAAGTTGCTGTCCTT | NGG | 2 | 0.2545 | Tier III |
40 | NC_000010.11 | 10 | 3529825 | - | LOC105376360 | NNNCAGCAGGTGCTGTCCTG | NGG | 2 | 0.2188 | Tier III |
41 | NC_000010.11 | 10 | 3529825 | - | LOC124902539 | NNNCAGCAGGTGCTGTCCTG | NGG | 2 | 0.2188 | Tier III |
42 | NC_000001.11 | 1 | 11149447 | - | MTOR-AS1 | NNNCAGAAGCTTCTGTCCTG | NGG | 2 | 0.2051 | Tier III |
43 | NC_000008.11 | 8 | 6656283 | + | MCPH1-AS1 | NNNCAGAAGGTTCTGTCCTG | NGG | 2 | 0.1923 | Tier III |
44 | NC_000005.10 | 5 | 135040409 | - | PITX1-AS1 | NNNCAGAAGTGTCTGTCCTG | NGG | 2 | 0.1538 | Tier III |
45 | NC_000002.12 | 2 | 75650905 | - | SUPT4H1P1 | NNNCAGAAGGTGCTGTCCAG | NGG | 2 | 0.1429 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000081.6 | 15 | 75451293 | + | Ly6l | NNNCAGAAGATGCTGTTCTG | NGG | 2 | 0.4 | Tier I |
2 | NC_000075.6 | 9 | 44514254 | + | Cxcr5 | NNNCAGAAGTTACTGTCCTG | NAG | 2 | 0.242 | Tier I |
3 | NC_000068.7 | 2 | 25264007 | - | Tprn | NNNCAGAAATTGCTGGCCTG | NGG | 2 | 0.2225 | Tier I |
4 | NC_000083.6 | 17 | 25389404 | - | Cacna1h | NNNCTGAAGTTGCTGGCCTG | NGG | 2 | 0.1259 | Tier I |
5 | NC_000075.6 | 9 | 14592792 | - | Amotl1 | NNNCAGAAGTGGCCGTCCTG | NGG | 2 | 0.1143 | Tier I |
6 | NC_000085.6 | 19 | 29373737 | - | Cd274 | NNNCTGAAGTTGCTGTGCTG | NGG | 2 | 0.0214 | Tier I |
7 | NC_000067.6 | 1 | 171277081 | - | Ppox | NNNCAGCAGTTGCTGTCCTG | NGC | 2 | 0.0097 | Tier I |
8 | NC_000071.6 | 5 | 117231119 | + | Taok3 | NNNCAGAAGAAGCTGTCCTG | NGG | 2 | 0.6429 | Tier II |
9 | NC_000081.6 | 15 | 99962056 | + | Fam186a | NNNTAGAAGTAGCTGTCCTG | NGG | 2 | 0.6 | Tier II |
10 | NC_000070.6 | 4 | 144786564 | + | Aadacl4fm5 | NNNAAGGAGTTGCTGTCCTG | NGG | 2 | 0.5944 | Tier II |
11 | NC_000083.6 | 17 | 3165740 | + | Scaf8 | NNNAAGAAGCTGCTGTCCTG | NGG | 2 | 0.4491 | Tier II |
12 | NC_000071.6 | 5 | 117250718 | - | Taok3 | NNNCAGATGTTGCTGACCTG | NGG | 2 | 0.3896 | Tier II |
13 | NC_000073.6 | 7 | 72083429 | + | Mctp2 | NNNCACAAGTTGCTGTCATG | NGG | 2 | 0.3671 | Tier II |
14 | NC_000075.6 | 9 | 121276978 | + | Ulk4 | NNNGATAAGTTGCTGTCCTG | NGG | 2 | 0.3333 | Tier II |
15 | NC_000071.6 | 5 | 118133379 | - | Fbxw8 | NNNCAGGAGTTGCTGTCCTC | NGG | 2 | 0.3025 | Tier II |
16 | NC_000072.6 | 6 | 39008179 | - | Tbxas1 | NNNCTTAAGTTGCTGTCCTG | NGG | 2 | 0.2424 | Tier II |
17 | NC_000067.6 | 1 | 172284418 | - | Atp1a2 | NNNCAGCAGCTGCTGTCCTG | NGG | 2 | 0.2333 | Tier II |
18 | NC_000085.6 | 19 | 31143155 | - | Prkg1 | NNNCAGTAGCTGCTGTCCTG | NGG | 2 | 0.2333 | Tier II |
19 | NC_000070.6 | 4 | 57465286 | - | Pakap | NNNCAGAAGCTGATGTCCTG | NGG | 2 | 0.2051 | Tier II |
20 | NC_000072.6 | 6 | 140463154 | + | Plekha5 | NNNGAGAAGTGGCTGTCCTG | NGG | 2 | 0.2 | Tier II |
21 | NC_000083.6 | 17 | 34684032 | - | Tnxb | NNNCAGAAGTGGCTGGCCTG | NGG | 2 | 0.1385 | Tier II |
22 | NC_000080.6 | 14 | 64725157 | + | Kif13b | NNNCAGAAGTTGCTGTACTG | NAG | 2 | 0.121 | Tier II |
23 | NC_000084.6 | 18 | 78133370 | - | Slc14a1 | NNNCAGGAGTTGCTTTCCTG | NGG | 2 | 0.1008 | Tier II |
24 | NC_000069.6 | 3 | 35811503 | + | Atp11b | NNNCAGAAGCTGCTTTCCTG | NGG | 2 | 0.0762 | Tier II |
25 | NC_000084.6 | 18 | 75222762 | + | Dym | NNNCAGAAGTTGCTGAGCTG | NGG | 2 | 0.0535 | Tier II |
26 | NC_000067.6 | 1 | 184962209 | - | Mark1 | NNNCAGAAGTTGCTGTCCTC | NTG | 2 | 0.0167 | Tier II |
27 | NC_000073.6 | 7 | 76663156 | - | Agbl1 | NNNCAGAAGTTGCTGTGCTG | NGC | 2 | 0.0013 | Tier II |
28 | NC_000073.6 | 7 | 127590010 | + | Rnf40 | NNNCAGAAGTTGCTGTCCTG | NAA | 2 | 0.0 | Tier II |
29 | NC_000078.6 | 12 | 24958126 | + | Mboat2 | NNNCAGAAGTTGCTGTCCTG | NAC | 2 | 0.0 | Tier II |
30 | NC_000078.6 | 12 | 3174271 | + | Gm9077 | NNNAAGAAGCTGCTGTCCTG | NGG | 2 | 0.4491 | Tier III |
31 | NC_000071.6 | 5 | 28642411 | - | 9530036O11Rik | NNNTAGAAGCTGCTGTCCTG | NGG | 2 | 0.4267 | Tier III |
32 | NC_000081.6 | 15 | 98937573 | + | Gm41396 | NNNCAGAAGCTGCAGTCCTG | NGG | 2 | 0.3302 | Tier III |
33 | NC_000067.6 | 1 | 172284418 | - | LOC115487369 | NNNCAGCAGCTGCTGTCCTG | NGG | 2 | 0.2333 | Tier III |
34 | NC_000084.6 | 18 | 26082330 | + | Gm33228 | NNNCAGTAGCTGCTGTCCTG | NGG | 2 | 0.2333 | Tier III |
35 | NC_000079.6 | 13 | 10543285 | + | Gm36525 | NNNCAGAAGTTGCTGGTCTG | NGG | 2 | 0.1615 | Tier III |
36 | NC_000079.6 | 13 | 10543285 | + | Gm26861 | NNNCAGAAGTTGCTGGTCTG | NGG | 2 | 0.1615 | Tier III |
37 | NC_000082.6 | 16 | 85356974 | - | Gm41483 | NNNCAAAAGTTGCTTTCCTG | NGG | 2 | 0.1429 | Tier III |
38 | NC_000083.6 | 17 | 25389404 | - | Gm33617 | NNNCTGAAGTTGCTGGCCTG | NGG | 2 | 0.1259 | Tier III |
39 | NC_000084.6 | 18 | 78133370 | - | Gm36338 | NNNCAGGAGTTGCTTTCCTG | NGG | 2 | 0.1008 | Tier III |
40 | NC_000067.6 | 1 | 79033139 | - | Gm29536 | NNNCAGAAGTTGCTGTCCTG | NAA | 2 | 0.0 | Tier III |