Construct: sgRNA BRDN0001149447

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGCAGAAGTTGCTGTCCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80259

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 42675729 + STARD9 NNNCAGAGGCTGCTGTCCTG NGG 2 0.3911 Tier I
2 NC_000005.10 5 56882666 + MAP3K1 NNNCAGAAGTTGCTGTCCTG NCT 2 0.0 Tier I
3 NC_000005.10 5 1336619 + CLPTM1L NNNCAGAACTTGCTGTCCTG NGG 1 0.5385 Tier II
4 NC_000019.10 19 47665391 + BICRA NNNCAAAAGTTCCTGTCCTG NGG 2 0.5294 Tier II
5 NC_000002.12 2 134447997 - MGAT5 NNNCAGAAGGTGCTGTCCTG NGG 1 0.5 Tier II
6 NC_000001.11 1 183102253 + LAMC1 NNNCAGAAGTTGCTGTTCTG NGG 1 0.4667 Tier II
7 NC_000020.11 20 9563652 + PAK5 NNNCAGAAATTGCTGCCCTG NGG 2 0.4286 Tier II
8 NC_000002.12 2 115360263 + DPP10 NNNCAGAAGTTGCTGTCATT NGG 2 0.3769 Tier II
9 NC_000010.11 10 116101135 - GFRA1 NNNCAGAAGGAGCTGTCCTG NGG 2 0.375 Tier II
10 NC_000016.10 16 81625287 + CMIP NNNCAGAAGCTGCAGTCCTG NGG 2 0.3302 Tier II
11 NC_000010.11 10 101580158 - POLL NNNCAGAAGTTCCAGTCCTG NGG 2 0.3277 Tier II
12 NC_000001.11 1 181556308 - CACNA1E NNNGAGAAATTGCTGTCCTG NGG 2 0.3214 Tier II
13 NC_000022.11 22 38326025 + TPTEP2-CSNK1E NNNCTGAAGATGCTGTCCTG NGG 2 0.3117 Tier II
14 NC_000007.14 7 158217466 - PTPRN2 NNNTTGAAGTTGCTGTCCTG NGG 2 0.2909 Tier II
15 NC_000010.11 10 51331023 + PRKG1 NNNCAGCAGTTGCAGTCCTG NGG 2 0.2708 Tier II
16 NC_000001.11 1 178767352 + RALGPS2 NNNCTGAAGTTGCTGTCCTT NGG 2 0.2545 Tier II
17 NC_000013.11 13 99311352 + UBAC2 NNNGAGAAGGTGCTGTCCTG NGG 2 0.25 Tier II
18 NC_000010.11 10 105219587 - SORCS3 NNNCTGAAATTGCTGTCCTG NGG 2 0.2338 Tier II
19 NC_000001.11 1 11149447 - MTOR NNNCAGAAGCTTCTGTCCTG NGG 2 0.2051 Tier II
20 NC_000001.11 1 243783422 - AKT3 NNNCAGAAGCTTCTGTCCTG NGG 2 0.2051 Tier II
21 NC_000006.12 6 34391130 - NUDT3 NNNCAGGAGTTGCTGTCCAG NGG 2 0.2017 Tier II
22 NC_000006.12 6 34391130 - RPS10-NUDT3 NNNCAGGAGTTGCTGTCCAG NGG 2 0.2017 Tier II
23 NC_000022.11 22 35417972 + MCM5 NNNCAGATGTTGCTGTTCTG NGG 2 0.2 Tier II
24 NC_000023.11 X 85055345 + APOOL NNNCAGCAGTGGCTGTCCTG NGG 2 0.175 Tier II
25 NC_000002.12 2 75650905 - MRPL19 NNNCAGAAGGTGCTGTCCAG NGG 2 0.1429 Tier II
26 NC_000010.11 10 101526493 + BTRC NNNCAGAAGTTGGTATCCTG NGG 2 0.1283 Tier II
27 NC_000006.12 6 146212043 + GRM1 NNNCAGAAGTTGCTGTCCAC NGG 2 0.1224 Tier II
28 NC_000015.10 15 71705537 + THSD4 NNNCAGATGTTGCTGTCCTG NAG 2 0.1111 Tier II
29 NC_000010.11 10 126108878 - ADAM12 NNNCAGAAGTTGCTGTCTTG NGA 2 0.0446 Tier II
30 NC_000012.12 12 51826688 - FIGNL2 NNNCAGAAGTTGCTGACCTG NTG 2 0.0354 Tier II
31 NC_000001.11 1 213603272 + RPS6KC1 NNNCAGAGGTTGCTGTCCTG NTG 2 0.0286 Tier II
32 NC_000020.11 20 16323820 + KIF16B NNNCAGAAGCTGCTGTCCTG NTG 2 0.0208 Tier II
33 NC_000003.12 3 70099939 + SAMMSON NNNCAGAAGTAACTGTCCTG NGG 2 0.7 Tier III
34 NC_000003.12 3 110658391 + LOC105374037 NNNCAGAAGCTGCTGACCTG NGG 2 0.4848 Tier III
35 NC_000010.11 10 5275276 - AKR1C7P NNNAAGAAGCTGCTGTCCTG NGG 2 0.4491 Tier III
36 NC_000020.11 20 9563652 + LOC105372523 NNNCAGAAATTGCTGCCCTG NGG 2 0.4286 Tier III
37 NC_000018.10 18 75439548 + LOC124900405 NNNCAGAAGTGGCTGTCCTG NGG 1 0.4 Tier III
38 NC_000011.10 11 30296314 - ARL14EP-DT NNNTTGAAGTTGCTGTCCTG NGG 2 0.2909 Tier III
39 NC_000011.10 11 34771917 + LOC102723568 NNNCTGAAGTTGCTGTCCTT NGG 2 0.2545 Tier III
40 NC_000010.11 10 3529825 - LOC105376360 NNNCAGCAGGTGCTGTCCTG NGG 2 0.2188 Tier III
41 NC_000010.11 10 3529825 - LOC124902539 NNNCAGCAGGTGCTGTCCTG NGG 2 0.2188 Tier III
42 NC_000001.11 1 11149447 - MTOR-AS1 NNNCAGAAGCTTCTGTCCTG NGG 2 0.2051 Tier III
43 NC_000008.11 8 6656283 + MCPH1-AS1 NNNCAGAAGGTTCTGTCCTG NGG 2 0.1923 Tier III
44 NC_000005.10 5 135040409 - PITX1-AS1 NNNCAGAAGTGTCTGTCCTG NGG 2 0.1538 Tier III
45 NC_000002.12 2 75650905 - SUPT4H1P1 NNNCAGAAGGTGCTGTCCAG NGG 2 0.1429 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000081.6 15 75451293 + Ly6l NNNCAGAAGATGCTGTTCTG NGG 2 0.4 Tier I
2 NC_000075.6 9 44514254 + Cxcr5 NNNCAGAAGTTACTGTCCTG NAG 2 0.242 Tier I
3 NC_000068.7 2 25264007 - Tprn NNNCAGAAATTGCTGGCCTG NGG 2 0.2225 Tier I
4 NC_000083.6 17 25389404 - Cacna1h NNNCTGAAGTTGCTGGCCTG NGG 2 0.1259 Tier I
5 NC_000075.6 9 14592792 - Amotl1 NNNCAGAAGTGGCCGTCCTG NGG 2 0.1143 Tier I
6 NC_000085.6 19 29373737 - Cd274 NNNCTGAAGTTGCTGTGCTG NGG 2 0.0214 Tier I
7 NC_000067.6 1 171277081 - Ppox NNNCAGCAGTTGCTGTCCTG NGC 2 0.0097 Tier I
8 NC_000071.6 5 117231119 + Taok3 NNNCAGAAGAAGCTGTCCTG NGG 2 0.6429 Tier II
9 NC_000081.6 15 99962056 + Fam186a NNNTAGAAGTAGCTGTCCTG NGG 2 0.6 Tier II
10 NC_000070.6 4 144786564 + Aadacl4fm5 NNNAAGGAGTTGCTGTCCTG NGG 2 0.5944 Tier II
11 NC_000083.6 17 3165740 + Scaf8 NNNAAGAAGCTGCTGTCCTG NGG 2 0.4491 Tier II
12 NC_000071.6 5 117250718 - Taok3 NNNCAGATGTTGCTGACCTG NGG 2 0.3896 Tier II
13 NC_000073.6 7 72083429 + Mctp2 NNNCACAAGTTGCTGTCATG NGG 2 0.3671 Tier II
14 NC_000075.6 9 121276978 + Ulk4 NNNGATAAGTTGCTGTCCTG NGG 2 0.3333 Tier II
15 NC_000071.6 5 118133379 - Fbxw8 NNNCAGGAGTTGCTGTCCTC NGG 2 0.3025 Tier II
16 NC_000072.6 6 39008179 - Tbxas1 NNNCTTAAGTTGCTGTCCTG NGG 2 0.2424 Tier II
17 NC_000067.6 1 172284418 - Atp1a2 NNNCAGCAGCTGCTGTCCTG NGG 2 0.2333 Tier II
18 NC_000085.6 19 31143155 - Prkg1 NNNCAGTAGCTGCTGTCCTG NGG 2 0.2333 Tier II
19 NC_000070.6 4 57465286 - Pakap NNNCAGAAGCTGATGTCCTG NGG 2 0.2051 Tier II
20 NC_000072.6 6 140463154 + Plekha5 NNNGAGAAGTGGCTGTCCTG NGG 2 0.2 Tier II
21 NC_000083.6 17 34684032 - Tnxb NNNCAGAAGTGGCTGGCCTG NGG 2 0.1385 Tier II
22 NC_000080.6 14 64725157 + Kif13b NNNCAGAAGTTGCTGTACTG NAG 2 0.121 Tier II
23 NC_000084.6 18 78133370 - Slc14a1 NNNCAGGAGTTGCTTTCCTG NGG 2 0.1008 Tier II
24 NC_000069.6 3 35811503 + Atp11b NNNCAGAAGCTGCTTTCCTG NGG 2 0.0762 Tier II
25 NC_000084.6 18 75222762 + Dym NNNCAGAAGTTGCTGAGCTG NGG 2 0.0535 Tier II
26 NC_000067.6 1 184962209 - Mark1 NNNCAGAAGTTGCTGTCCTC NTG 2 0.0167 Tier II
27 NC_000073.6 7 76663156 - Agbl1 NNNCAGAAGTTGCTGTGCTG NGC 2 0.0013 Tier II
28 NC_000073.6 7 127590010 + Rnf40 NNNCAGAAGTTGCTGTCCTG NAA 2 0.0 Tier II
29 NC_000078.6 12 24958126 + Mboat2 NNNCAGAAGTTGCTGTCCTG NAC 2 0.0 Tier II
30 NC_000078.6 12 3174271 + Gm9077 NNNAAGAAGCTGCTGTCCTG NGG 2 0.4491 Tier III
31 NC_000071.6 5 28642411 - 9530036O11Rik NNNTAGAAGCTGCTGTCCTG NGG 2 0.4267 Tier III
32 NC_000081.6 15 98937573 + Gm41396 NNNCAGAAGCTGCAGTCCTG NGG 2 0.3302 Tier III
33 NC_000067.6 1 172284418 - LOC115487369 NNNCAGCAGCTGCTGTCCTG NGG 2 0.2333 Tier III
34 NC_000084.6 18 26082330 + Gm33228 NNNCAGTAGCTGCTGTCCTG NGG 2 0.2333 Tier III
35 NC_000079.6 13 10543285 + Gm36525 NNNCAGAAGTTGCTGGTCTG NGG 2 0.1615 Tier III
36 NC_000079.6 13 10543285 + Gm26861 NNNCAGAAGTTGCTGGTCTG NGG 2 0.1615 Tier III
37 NC_000082.6 16 85356974 - Gm41483 NNNCAAAAGTTGCTTTCCTG NGG 2 0.1429 Tier III
38 NC_000083.6 17 25389404 - Gm33617 NNNCTGAAGTTGCTGGCCTG NGG 2 0.1259 Tier III
39 NC_000084.6 18 78133370 - Gm36338 NNNCAGGAGTTGCTTTCCTG NGG 2 0.1008 Tier III
40 NC_000067.6 1 79033139 - Gm29536 NNNCAGAAGTTGCTGTCCTG NAA 2 0.0 Tier III
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Other clones with same target sequence:

(none)