Construct: ORF TRCN0000465220

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009163.1_s317c1
Derived from:
ccsbBroadEn_15129
DNA Barcode:
ATAAAGCACCCGCTCCGACAGGGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CELF6 (60677)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465220
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 60677 CELF6 CUGBP Elav-like family memb... NM_001172685.1 90.8% 88.9% (many diffs)
2 human 60677 CELF6 CUGBP Elav-like family memb... NM_052840.5 75.1% 72.4% (many diffs)
3 human 60677 CELF6 CUGBP Elav-like family memb... NM_001172684.1 69.5% 66.8% (many diffs)
4 mouse 76183 Celf6 CUGBP, Elav-like family mem... XM_006511543.2 84.6% 90% (many diffs)
5 mouse 76183 Celf6 CUGBP, Elav-like family mem... XM_006511544.2 84.6% 90% (many diffs)
6 mouse 76183 Celf6 CUGBP, Elav-like family mem... XM_006511545.2 84.6% 90% (many diffs)
7 mouse 76183 Celf6 CUGBP, Elav-like family mem... XM_011242834.2 84.6% 90% (many diffs)
8 mouse 76183 Celf6 CUGBP, Elav-like family mem... XM_017313681.1 81.6% 88% (many diffs)
9 mouse 76183 Celf6 CUGBP, Elav-like family mem... XM_006511546.2 79.7% 85.1% (many diffs)
10 mouse 76183 Celf6 CUGBP, Elav-like family mem... NM_001311092.1 79.4% 84.9% (many diffs)
11 mouse 76183 Celf6 CUGBP, Elav-like family mem... XM_006511540.3 64.8% 68.7% (many diffs)
12 mouse 76183 Celf6 CUGBP, Elav-like family mem... NM_175235.3 60.9% 65% (many diffs)
13 mouse 76183 Celf6 CUGBP, Elav-like family mem... XM_006511541.3 60.7% 64.8% (many diffs)
14 mouse 76183 Celf6 CUGBP, Elav-like family mem... XM_006511542.3 52.7% 56.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1170
ORF length:
1104
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat ctcagatccc ccctcaaaca acagctggaa ctctgttctc tatcttctag
121aggaccgaaa gctgtttgtg gggatgctgg gcaagcagca gggtgaggag gacgtcagac
181gcctgttcca gccctttggc cacatcgagg agtgcacggt cctgcggagt cctgacggca
241ccagtaaagg ctgtgccttt gtgaagttcg ggagtcaagg ggaagctcag gcggccatcc
301ggggtctgca cggcagccgg accatggcgg gcgcctcgtc cagcctcgtg gtcaagctgg
361cggacaccga ccgggagcgc gcgctgcggc ggatgcagca gatggccggc cacctgggcg
421ccttccaccc cgcgccactg ccgctagggg cctgcggcgc ctacaccacg gcgatcctgc
481agcaccaggc ggccctgctg gcggcggcac agggcccagg cctaggcccg gtggcggcag
541tggcggccca gatgcaacac gtggcggcct ttagcctggt agctgcgcct ctgttgcccg
601cggcagcagc caactccccg cctggcagcg gccctggcac cctcccaggt cttccggcgc
661ccatcggggt caatggattc ggccctctga ccccccagac caatggccag ccgggctccg
721acacgctcta caataacggg ctctcccctt atccagccca gagccccggc gtggctgacc
781ccctgcagca ggcctacgct gggatgcacc actacgcagc agcctatccg tcggcctatg
841ccccagtgag cacagctttt ccccagcagc cttcagccct gccccagcag cagagagaag
901gccccgaagg ctgtaacctc ttcatctatc acctgcctca ggagtttggt gatgcggaac
961tcatacagac attcctgccc tttGGAGCCG TTGTCTCTGC TAAAGTCTTT GTGGATCGAG
1021CCACCAACCA GAGCAAGTGT TTTGGGTTTG TTAGTTTTGA CAATCCAACT AGTGCCCAGA
1081CTGCTATTCA GGCGATGAAT GGCTTTCAAA TTGGCATGAA GAGGCTCAAG GTCCAGCTAA
1141AGCGGCCCAA GGATGCCAAC CGGCCTTACT GCCCAACTTT CTTGTACAAA GTGGTTGATA
1201TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC
1261CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAATAAA
1321GCACCCGCTC CGACAGGGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg
1381tgaaagatt

Download FASTA (ORF) (Full)