Construct: ORF TRCN0000465225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006153.1_s317c1
- Derived from:
- ccsbBroadEn_12039
- DNA Barcode:
- GTCGCAGCGTATTGCCTGGATTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANLN (54443)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465225
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54443 | ANLN | anillin actin binding protein | XM_017012356.2 | 37.3% | 37.3% | 1_2034del |
| 2 | human | 54443 | ANLN | anillin actin binding protein | NM_001284302.3 | 37.2% | 37.2% | 1_2043del |
| 3 | human | 54443 | ANLN | anillin actin binding protein | NM_001284301.3 | 37.2% | 37.2% | 1_2046del |
| 4 | human | 54443 | ANLN | anillin actin binding protein | XM_006715747.4 | 36.6% | 36.6% | 1_2046del;2770_2823del |
| 5 | human | 54443 | ANLN | anillin actin binding protein | XM_017012355.2 | 36.1% | 36.1% | 1_2145del |
| 6 | human | 54443 | ANLN | anillin actin binding protein | NM_018685.5 | 36% | 36% | 1_2157del |
| 7 | human | 54443 | ANLN | anillin actin binding protein | XM_017012354.2 | 35.5% | 35.5% | 1_2145del;2869_2922del |
| 8 | human | 54443 | ANLN | anillin actin binding protein | XM_006715746.2 | 35.4% | 35.4% | 1_2157del;2881_2934del |
| 9 | mouse | 68743 | Anln | anillin, actin binding protein | XM_011242594.1 | 54.9% | 56.3% | (many diffs) |
| 10 | mouse | 68743 | Anln | anillin, actin binding protein | NM_028390.3 | 31.5% | 32.3% | (many diffs) |
| 11 | mouse | 68743 | Anln | anillin, actin binding protein | XM_006510581.3 | 31% | 31.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1281
- ORF length:
- 1215
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggaactcaat aacgaaataa atatgcaaca gacagtgatc tatcaagcta 121 gccaggctct taactgctgt gttgatgaag aacatggaaa agggtcccta gaagaagctg 181 aagcagaaag acttcttcta attgcaactg ggaagagaac acttttgatt gatgaattga 241 ataaattgaa gaacgaagga cctcagagga agaataaggc tagtccccaa agtgaattta 301 tgccatccaa aggatcagtt actttgtcag aaatccgctt gcctctaaaa gcagattttg 361 tctgcagtac ggttcagaaa ccagatgcag caaattacta ttacttaatt atactaaaag 421 caggagctga aaatatggta gccacaccat tagcaagtac ttcaaactct cttaacggtg 481 atgctctgac attcactact acatttactc tgcaagatgt atccaatgac tttgaaataa 541 atattgaagt ttacagcttg gtgcaaaaga aagatccctc aggccttgat aagaagaaaa 601 aaacatccaa gtccaaggct attactccaa agcgactcct cacatctata accacaaaaa 661 gcaacattca ttcttcagtc atggccagtc caggaggtct tagtgctgtg cgaaccagca 721 acttcgccct tgttggatct tacacattat cattgtcttc agtaggaaat actaagtttg 781 ttctggacaa ggtccccttt ttatcttctt tggaaggtca tatttattta aaaataaaat 841 gtcaagtgaa ttccagtgtt gaagaaagag gttttctaac catatttgaa gatgttagtg 901 gttttggtgc ctggcatcga agatggtgtg ttctttctgg aaactgtata tcttattgga 961 cttatccaga tgatgagaaa cgcaagaatc ccataggaag gataaatctg gctaattgta 1021 ccagtcgtca gataGAACCA GCCAACAGAG AATTTTGTGC AAGACGCAAC ACTTTTGAAT 1081 TAATTACTGT CCGACCACAA AGAGAAGATG ACCGAGAGAC TCTTGTCAGC CAATGCAGGG 1141 ACACACTCTG TGTTACCAAG AACTGGCTGT CTGCAGATAC TAAAGAAGAG CGGGATCTCT 1201 GGATGCAAAA ACTCAATCAA GTTCTTGTTG ATATTCGCCT CTGGCAACCT GATGCTTGCT 1261 ACAAACCTAT TGGAAAGCCT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1321 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1381 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTCG CAGCGTATTG 1441 CCTGGATTGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt