Construct: ORF TRCN0000465253
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011132.1_s317c1
- Derived from:
- ccsbBroadEn_06611
- DNA Barcode:
- CTCCACCCCGAGGCCGGGTTTTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYO1C (4641)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465253
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4641 | MYO1C | myosin IC | NM_033375.5 | 99.9% | 99.8% | 2278G>A;2372A>G |
| 2 | human | 4641 | MYO1C | myosin IC | XM_024450768.1 | 99.9% | 99.8% | 2278G>A;2372A>G |
| 3 | human | 4641 | MYO1C | myosin IC | XM_024450769.1 | 99.9% | 99.8% | 2278G>A;2372A>G |
| 4 | human | 4641 | MYO1C | myosin IC | NM_001363855.1 | 98.8% | 98.7% | 1_33del;2311G>A;2405A>G |
| 5 | human | 4641 | MYO1C | myosin IC | NM_001080950.2 | 98.4% | 98.2% | 1_48del;2326G>A;2420A>G |
| 6 | human | 4641 | MYO1C | myosin IC | NM_001080779.2 | 96.6% | 96.5% | 1_105del;2383G>A;2477A>G |
| 7 | mouse | 17913 | Myo1c | myosin IC | NM_001080774.1 | 88.7% | 96.8% | (many diffs) |
| 8 | mouse | 17913 | Myo1c | myosin IC | NM_008659.3 | 88.7% | 96.8% | (many diffs) |
| 9 | mouse | 17913 | Myo1c | myosin IC | NM_001080775.1 | 87.3% | 95.4% | (many diffs) |
| 10 | mouse | 17913 | Myo1c | myosin IC | XM_006532429.3 | 85.8% | 93.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3150
- ORF length:
- 3084
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gagtgcgctc accgcccgtg accgggtggg ggtgcaggat ttcgtgctgc 121 tggagaactt caccagcgag gccgccttca tcgagaacct gcggcggcga tttcgggaga 181 atctcatcta cacctacatt ggccccgtcc tggtctctgt caatccctac cgggacctgc 241 agatctacag ccggcagcat atggagcgtt accgtggcgt cagcttctat gaagtgcccc 301 ctcacctgtt tgccgtggcg gacactgtgt accgagcact gcgcacggag cgtcgggacc 361 aggctgtgat gatctctggg gagagcgggg caggcaagac cgaggccacc aagaggctgc 421 tgcagttcta tgcagagacc tgcccagccc ccgagcgcgg aggtgccgtg cgggaccggc 481 tgctacagag caacccggtg ctggaggcct ttggaaatgc caagaccctc cggaacgata 541 actccagcag gttcgggaag tacatggatg tgcagtttga cttcaagggt gcccccgtgg 601 gtggccacat cctcagttac ctcctggaaa agtcacgagt ggtgcaccag aatcatgggg 661 agcggaactt ccacatcttc taccagctgc tggagggggg cgaggaggag actcttcgca 721 ggctgggctt ggaacggaac ccccagagct acctgtacct ggtgaagggc cagtgtgcca 781 aagtctcctc catcaacgac aagagtgact ggaaggtcgt caggaaggct ctgacagtca 841 ttgatttcac cgaggatgaa gtggaggacc tgctgagcat cgtggccagc gtccttcatt 901 tgggcaacat ccactttgct gccaacgagg agagcaatgc ccaggtcacc accgagaacc 961 agctcaagta tctgaccagg ctcctcagcg tggaaggctc gacgctgcga gaagccctga 1021 cacacaggaa gatcatcgcc aagggggagg agctcctgag cccgctgaac ctggagcagg 1081 ccgcgtacgc acgagacgcc ctcgccaagg ctgtgtacag ccgcactttt acctggctcg 1141 tcgggaagat caacaggtcg ctggcctcca aggacgtgga gagccccagc tggcggagca 1201 ccacggttct cgggctcctg gatatttatg gctttgaagt gtttcagcat aacagctttg 1261 agcagttctg catcaattac tgcaacgaga agctgcagca gctcttcatc gagctcacgc 1321 tcaagtcgga gcaggaggag tacgaggcag agggcatcgc gtgggagccc gtccagtatt 1381 tcaacaacaa aatcatctgt gatctggtgg aggagaagtt taagggcatc atctcgattt 1441 tggatgagga gtgtctgcgc cccggggagg ccacagacct gaccttcctg gagaagctgg 1501 aggatactgt caagcaccat ccacacttcc tgacgcacaa gctggctgac cagcggacca 1561 ggaaatctct gggccgaggg gaattccgcc ttctgcacta tgcgggggag gtgacctaca 1621 gcgtgaccgg gtttctggac aaaaacaatg accttctctt ccggaacctt aaggagacca 1681 tgtgtagctc aaagaatccc attatgagcc agtgctttga ccggagcgag ctcagtgaca 1741 agaagcggcc agagacggtc gccacccagt tcaagatgag cctcctgcag ctggtggaga 1801 tcctgcagtc taaggagccc gcctacgtcc gctgcatcaa acccaatgat gccaaacagc 1861 ccggccgctt tgacgaggtg ctgatccgcc accaggtgaa gtacctgggg ctgttggaaa 1921 acctgcgcgt gcgcagagcc ggctttgcct atcgccgcaa atacgaagct ttcctgcaaa 1981 ggtacaagtc actgtgccca gagacgtggc ccacgtgggc aggacggccg caggatgggg 2041 tggctgtgct ggtccgacac ctgggctaca agccagaaga gtacaagatg ggcaggacca 2101 agatcttcat ccgcttcccc aagaccctgt ttgccacaga ggatgccctg gaggtccggc 2161 ggcagagcct ggccacaaag atccaagctg cctggagggg ctttcactgg cggcagaaat 2221 tcctccgggt gaagagatca gccatctgca tccagtcgtg gtggcgtgga acactgggcc 2281 ggaggaaggc agccaagagg aagtgggcgg cacagaccat ccggcggctc atccgaggct 2341 tcatcctgcg ccacgccccc cgctgccccg agaacgcctt cttcctggac catgtgcgca 2401 cctctttttt gctaaacctg aggcggcagc tgccccggaa tgtcctggac acctcgtggc 2461 ccacgccccc acctgccctg cgtgaggcct cagagcttct gcgggagttg tgcataaaga 2521 acatggtgtg gaaatactgc cggagtatca gccctgagtg gaagcagcag ctgcagcaga 2581 aggccgtggc tagtgagatc ttcaagggca agaaggataa ttaccctcag agtgtaccca 2641 ggctcttcat cagcactcgg cttggtacag atgagatcag cccccgagtg ctgcaggcct 2701 tgggctctga gcccattcag tatgcggtgc ctgttgtgaa atacgaccgc aagggctaca 2761 agcctcgctc ccggcagctg ctgctcacgc ccaacgccgt cgtcatcgtg gaggacgcca 2821 aagtcaagca gaggattgat tacgccaacc tgaccggaat ctctgtcagc agccTGAGCG 2881 ACAGTCTTTT TGTGCTTCAT GTACAGCGTG CGGACAATAA GCAAAAGGGA GATGTGGTGC 2941 TGCAGAGTGA CCACGTGATT GAGACGCTGA CCAAGACAGC CCTCAGTGCC AACCGCGTGA 3001 ACAGCATCAA CATCAACCAG GGCAGCATCA CGTTTGCAGG GGGCCCCGGC AGGGATGGCA 3061 CCATTGACTT CACACCCGGC TCGGAGCTGC TCATCACCAA GGCCAAGAAC GGGCACCTGG 3121 CTGTGGTCGC CCCACGGCTG AATTCTCGGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 3181 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 3241 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTCCA 3301 CCCCGAGGCC GGGTTTTAGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 3361 tgaaagatt