Transcript: Mouse XM_006532429.3

PREDICTED: Mus musculus myosin IC (Myo1c), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Myo1c (17913)
Length:
4627
CDS:
162..3353

Additional Resources:

NCBI RefSeq record:
XM_006532429.3
NBCI Gene record:
Myo1c (17913)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006532429.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000100741 CCGCGTGAACAATATCAACAT pLKO.1 3194 CDS 100% 4.950 6.930 N Myo1c n/a
2 TRCN0000335217 CCGCGTGAACAATATCAACAT pLKO_005 3194 CDS 100% 4.950 6.930 N Myo1c n/a
3 TRCN0000348590 CCCTCTGCTGATGCCAAATAT pLKO_005 3595 3UTR 100% 15.000 10.500 N Myo1c n/a
4 TRCN0000100743 GAATTGATTATGCCAACCTAA pLKO.1 3034 CDS 100% 4.950 3.465 N Myo1c n/a
5 TRCN0000100744 GACAACAAGCAGAAGGGAGAT pLKO.1 3114 CDS 100% 4.050 2.835 N Myo1c n/a
6 TRCN0000335137 GACAACAAGCAGAAGGGAGAT pLKO_005 3114 CDS 100% 4.050 2.835 N Myo1c n/a
7 TRCN0000100740 CGACCCAATTTAAGAATGGTA pLKO.1 4387 3UTR 100% 3.000 2.100 N Myo1c n/a
8 TRCN0000100742 GCTTTGCCTATCGTCGCAAAT pLKO.1 2143 CDS 100% 1.080 0.756 N Myo1c n/a
9 TRCN0000335215 GCTTTGCCTATCGTCGCAAAT pLKO_005 2143 CDS 100% 1.080 0.756 N Myo1c n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006532429.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06611 pDONR223 100% 85.8% 93.6% None (many diffs) n/a
2 ccsbBroad304_06611 pLX_304 0% 85.8% 93.6% V5 (many diffs) n/a
3 TRCN0000465253 CTCCACCCCGAGGCCGGGTTTTAG pLX_317 9.2% 85.8% 93.6% V5 (many diffs) n/a
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