Construct: ORF TRCN0000465260
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009599.1_s317c1
- Derived from:
- ccsbBroadEn_02073
- DNA Barcode:
- CCGTCCAGCCGGTATACATTTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLDN10 (9071)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465260
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9071 | CLDN10 | claudin 10 | NM_006984.5 | 100% | 100% | |
| 2 | human | 9071 | CLDN10 | claudin 10 | XM_011521134.2 | 88.3% | 88.3% | 220_309del |
| 3 | human | 9071 | CLDN10 | claudin 10 | NM_182848.3 | 81% | 75.5% | (many diffs) |
| 4 | human | 9071 | CLDN10 | claudin 10 | NM_001160100.1 | 79.4% | 74.6% | (many diffs) |
| 5 | human | 9071 | CLDN10 | claudin 10 | XM_017020844.1 | 74.8% | 67.5% | (many diffs) |
| 6 | human | 9071 | CLDN10 | claudin 10 | XM_017020843.1 | 73% | 64.9% | (many diffs) |
| 7 | human | 9071 | CLDN10 | claudin 10 | XM_024449432.1 | 64% | 64% | 0_1ins246 |
| 8 | mouse | 58187 | Cldn10 | claudin 10 | NM_021386.4 | 87.1% | 92.6% | (many diffs) |
| 9 | mouse | 58187 | Cldn10 | claudin 10 | NM_001160099.1 | 74.8% | 78.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 750
- ORF length:
- 684
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tagcacggct tcggagatca tcgccttcat ggtctccatc tcaggctggg 121 tactggtgtc ctccacgctg cccaccgact actggaaggt gtctaccatc gacggcacgg 181 tcatcacaac cgccacctat tgggccaacc tgtggaaggc gtgcgttacc gactccacgg 241 gcgtctccaa ctgcaaggac ttcccctcca tgctggcgct ggacggttat atacaggcat 301 gtagaggact tatgatcgct gctgtcagcc tgggcttctt tggttccata tttgcgctct 361 ttggaatgaa gtgtaccaaa gtcggaggct ccgataaagc caaagctaaa attgcttgtt 421 tGGCTGGGAT TGTATTCATA CTGTCAGGGC TGTGCTCAAT GACTGGATGT TCCCTATATG 481 CAAACAAAAT CACAACGGAA TTCTTTGATC CTCTCTTTGT TGAGCAAAAG TATGAATTAG 541 GAGCCGCTCT GTTTATTGGA TGGGCAGGAG CCTCACTGTG CATAATTGGT GGTGTCATAT 601 TTTGCTTTTC AATATCTGAC AACAACAAAA CACCCAGATA CACATACAAC GGGGCCACAT 661 CTGTCATGTC TTCTCGGACA AAGTATCATG GTGGAGAAGA TTTTAAAACA ACAAACCCTT 721 CAAAACAGTT TGATAAAAAT GCTTATGTCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 781 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 841 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCGTC 901 CAGCCGGTAT ACATTTAAAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 961 tgaaagatt