Construct: ORF TRCN0000465265
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007635.1_s317c1
- Derived from:
- ccsbBroadEn_01261
- DNA Barcode:
- TCGCCCCGCTTATTGGGACAGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1R8 (5511)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465265
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5511 | PPP1R8 | protein phosphatase 1 regul... | NM_138558.3 | 100% | 100% | |
| 2 | human | 5511 | PPP1R8 | protein phosphatase 1 regul... | NM_002713.4 | 60.7% | 60.7% | 0_1ins246 |
| 3 | human | 5511 | PPP1R8 | protein phosphatase 1 regul... | XM_017001594.2 | 60.7% | 60.7% | 0_1ins246 |
| 4 | human | 5511 | PPP1R8 | protein phosphatase 1 regul... | NM_014110.5 | 59.5% | 59.5% | 1_426del |
| 5 | mouse | 100336 | Ppp1r8 | protein phosphatase 1, regu... | NM_146154.3 | 54% | 57.8% | (many diffs) |
| 6 | mouse | 100336 | Ppp1r8 | protein phosphatase 1, regu... | NM_001290725.1 | 53.7% | 57.5% | (many diffs) |
| 7 | mouse | 100336 | Ppp1r8 | protein phosphatase 1, regu... | XM_006538464.3 | 50.3% | 53.8% | (many diffs) |
| 8 | mouse | 100336 | Ppp1r8 | protein phosphatase 1, regu... | XM_006538465.3 | 50% | 53.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tggagaggat gatgaactca agggcttact ggggcttcca gaggaggaaa 121 ctgagcttga taacctgaca gagttcaaca ctgcccacaa caagcggatt tctaccctta 181 ccattgagga gggaaatctg gacattcaaa gaccaaagag gaagaggaag aactcacggg 241 tgacattcag tgaggatgat gagatcatca acccagagga tgtggatccc tcagttggtc 301 gattcaggaa catggtgcaa actgcagtgg tcccagtcaa gaagaagcgt gtggagggcc 361 ctggctccct gggcctggag gaatcaggga gcaggcgcat gcagaacttt gccttcagcg 421 gaggactcta cgggggcctg ccccccacaC ACAGTGAAGC AGGCTCCCAG CCACATGGCA 481 TCCATGGGAC AGCACTCATC GGTGGCTTGC CCATGCCATA CCCAAACCTT GCCCCTGATG 541 TGGACTTGAC TCCTGTTGTG CCGTCAGCAG TGAACATGAA CCCTGCACCA AACCCTGCAG 601 TCTATAACCC TGAAGCTGTA AATGAACCCA AGAAGAAGAA ATATGCAAAA GAGGCTTGGC 661 CAGGCAAGAA GCCCACACCT TCCTTGCTGA TTTGCCCAAC TTTCTTGTAC AAAGTGGTTG 721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 841 GCCCCGCTTA TTGGGACAGC GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 901 ttgtgaaaga tt