Construct: ORF TRCN0000465267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015749.1_s317c1
- Derived from:
- ccsbBroadEn_00645
- DNA Barcode:
- TGCACAATCACATTTAATCCTCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCLM (2730)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465267
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2730 | GCLM | glutamate-cysteine ligase m... | NM_002061.4 | 100% | 100% | |
| 2 | human | 2730 | GCLM | glutamate-cysteine ligase m... | NM_001308253.1 | 91.9% | 91.9% | 125_126ins66 |
| 3 | human | 2730 | GCLM | glutamate-cysteine ligase m... | XM_017001056.2 | 84.5% | 81% | (many diffs) |
| 4 | human | 2730 | GCLM | glutamate-cysteine ligase m... | XM_017001057.2 | 77% | 73.2% | (many diffs) |
| 5 | human | 2730 | GCLM | glutamate-cysteine ligase m... | XM_011541261.2 | 67.8% | 67.8% | 0_1ins264 |
| 6 | mouse | 14630 | Gclm | glutamate-cysteine ligase, ... | NM_008129.4 | 91.3% | 96.3% | (many diffs) |
| 7 | mouse | 14630 | Gclm | glutamate-cysteine ligase, ... | XM_030252423.1 | 35.3% | 32.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 888
- ORF length:
- 822
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg caccgacagc cgcgcggcca aggcgctcct ggcgcgggcc cgcaccctgc 121 acctgcagac ggggaacctg ctgaactggg gccgcctgcg gaagaagtgc ccgtccacgc 181 acagcgagga gcttcatgat tgtatccaaa aaaccttgaa tgaatggagt tcccaaatca 241 acccagattt ggtcagggag tttccagatg tcttggaatg cactgtatct catgcagtag 301 aaaagataaa tcctgatgaa agagaagaaa tgaaagtttc tgcaaaactg ttcattgtag 361 aatcaaactc ttcatcatca actagaagtg cagttgacat ggcctgttca gtccttggag 421 ttgcacagct ggattctgtg atcattgctt cacctcctat tgaagatgga gttaatcttt 481 ccttggagca tttacagcct tactgggagg aattagaaaa cttagttcag agcaaaaaga 541 ttgttgccat aggtacctct gatctagaca aaacacagtt ggaacagctg tatcagtggg 601 cacaggtaaa accaaatagt aaccaagtta atcttgcctc ctgctgtgtg atgccaccag 661 atttgactgc atttgctaaa caatttgaca tacagctgtt gactcacaat gatccaaaag 721 aactgctttc tgaagcaagt ttccaagaag ctcttcagga aagcattcct gacattcaag 781 cgcacgagtg ggtgccgctg tggctactgc ggtattcggt cattgtgaaa agtagaggaa 841 ttatcaaatc aaaaggctac attttacaag ctaaaagaag gggttcTTAC CCAACTTTCT 901 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 961 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1021 GTGGAAAGGA CGATGCACAA TCACATTTAA TCCTCGTACG CGTTAAGTCg acaatcaacc 1081 tctggattac aaaatttgtg aaagatt