Transcript: Human XM_017001056.2

PREDICTED: Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GCLM (2730)
Length:
6009
CDS:
1201..2028

Additional Resources:

NCBI RefSeq record:
XM_017001056.2
NBCI Gene record:
GCLM (2730)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017001056.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000296747 CCAAATAGTAACCAAGTTAAT pLKO_005 1747 CDS 100% 13.200 18.480 N GCLM n/a
2 TRCN0000120831 GTTAATCTTTCCTTGGAGCAT pLKO.1 1606 CDS 100% 2.640 3.696 N Gclm n/a
3 TRCN0000345313 GTTAATCTTTCCTTGGAGCAT pLKO_005 1606 CDS 100% 2.640 3.696 N Gclm n/a
4 TRCN0000048491 GCAAGTTTCCAAGAAGCTCTT pLKO.1 2072 3UTR 100% 4.050 3.240 N GCLM n/a
5 TRCN0000290800 GCAAGTTTCCAAGAAGCTCTT pLKO_005 2072 3UTR 100% 4.050 3.240 N GCLM n/a
6 TRCN0000296748 GCAGATATTCTTTCGTCATAT pLKO_005 2326 3UTR 100% 13.200 9.240 N GCLM n/a
7 TRCN0000048489 CCACCAGATTTGACTGCATTT pLKO.1 1789 CDS 100% 10.800 7.560 N GCLM n/a
8 TRCN0000290874 CCACCAGATTTGACTGCATTT pLKO_005 1789 CDS 100% 10.800 7.560 N GCLM n/a
9 TRCN0000048488 CCAGATTTGGTCAGGGAGTTT pLKO.1 1378 CDS 100% 4.950 3.465 N GCLM n/a
10 TRCN0000048490 CCTCCTATTGAAGATGGAGTT pLKO.1 1588 CDS 100% 4.050 2.835 N GCLM n/a
11 TRCN0000290799 CCTCCTATTGAAGATGGAGTT pLKO_005 1588 CDS 100% 4.050 2.835 N GCLM n/a
12 TRCN0000048492 CCTTGGAGCATTTACAGCCTT pLKO.1 1616 CDS 100% 2.640 1.848 N GCLM n/a
13 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 3469 3UTR 100% 4.950 2.475 Y CFLAR n/a
14 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 3469 3UTR 100% 4.950 2.475 Y C19orf31 n/a
15 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 3467 3UTR 100% 4.950 2.475 Y ERN2 n/a
16 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 3467 3UTR 100% 4.950 2.475 Y P3H4 n/a
17 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 3467 3UTR 100% 4.950 2.475 Y P3H4 n/a
18 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 1855 CDS 100% 4.950 2.475 Y n/a
19 TRCN0000107105 CGAGGTCAGGAGTTTGAGATT pLKO.1 3513 3UTR 100% 4.950 2.475 Y NLRP12 n/a
20 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 3633 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017001056.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00645 pDONR223 100% 84.5% 81% None (many diffs) n/a
2 ccsbBroad304_00645 pLX_304 0% 84.5% 81% V5 (many diffs) n/a
3 TRCN0000465267 TGCACAATCACATTTAATCCTCGT pLX_317 1.4% 84.5% 81% V5 (many diffs) n/a
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