Transcript: Human NM_001308253.1

Homo sapiens glutamate-cysteine ligase modifier subunit (GCLM), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-07-06
Taxon:
Homo sapiens (human)
Gene:
GCLM (2730)
Length:
5000
CDS:
436..1194

Additional Resources:

NCBI RefSeq record:
NM_001308253.1
NBCI Gene record:
GCLM (2730)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001308253.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000296747 CCAAATAGTAACCAAGTTAAT pLKO_005 916 CDS 100% 13.200 18.480 N GCLM n/a
2 TRCN0000120831 GTTAATCTTTCCTTGGAGCAT pLKO.1 775 CDS 100% 2.640 3.696 N Gclm n/a
3 TRCN0000345313 GTTAATCTTTCCTTGGAGCAT pLKO_005 775 CDS 100% 2.640 3.696 N Gclm n/a
4 TRCN0000048491 GCAAGTTTCCAAGAAGCTCTT pLKO.1 1039 CDS 100% 4.050 3.240 N GCLM n/a
5 TRCN0000290800 GCAAGTTTCCAAGAAGCTCTT pLKO_005 1039 CDS 100% 4.050 3.240 N GCLM n/a
6 TRCN0000296748 GCAGATATTCTTTCGTCATAT pLKO_005 1293 3UTR 100% 13.200 9.240 N GCLM n/a
7 TRCN0000048489 CCACCAGATTTGACTGCATTT pLKO.1 958 CDS 100% 10.800 7.560 N GCLM n/a
8 TRCN0000290874 CCACCAGATTTGACTGCATTT pLKO_005 958 CDS 100% 10.800 7.560 N GCLM n/a
9 TRCN0000048490 CCTCCTATTGAAGATGGAGTT pLKO.1 757 CDS 100% 4.050 2.835 N GCLM n/a
10 TRCN0000290799 CCTCCTATTGAAGATGGAGTT pLKO_005 757 CDS 100% 4.050 2.835 N GCLM n/a
11 TRCN0000048492 CCTTGGAGCATTTACAGCCTT pLKO.1 785 CDS 100% 2.640 1.848 N GCLM n/a
12 TRCN0000007228 CACCTGTAATCCCAGCACTTT pLKO.1 2436 3UTR 100% 4.950 2.475 Y CFLAR n/a
13 TRCN0000166635 CACCTGTAATCCCAGCACTTT pLKO.1 2436 3UTR 100% 4.950 2.475 Y C19orf31 n/a
14 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 2434 3UTR 100% 4.950 2.475 Y ERN2 n/a
15 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 2434 3UTR 100% 4.950 2.475 Y P3H4 n/a
16 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 2434 3UTR 100% 4.950 2.475 Y P3H4 n/a
17 TRCN0000107105 CGAGGTCAGGAGTTTGAGATT pLKO.1 2480 3UTR 100% 4.950 2.475 Y NLRP12 n/a
18 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 2600 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001308253.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00645 pDONR223 100% 91.9% 91.9% None 125_126ins66 n/a
2 ccsbBroad304_00645 pLX_304 0% 91.9% 91.9% V5 125_126ins66 n/a
3 TRCN0000465267 TGCACAATCACATTTAATCCTCGT pLX_317 1.4% 91.9% 91.9% V5 125_126ins66 n/a
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