Construct: ORF TRCN0000465280
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000244.1_s317c1
- Derived from:
- ccsbBroadEn_11889
- DNA Barcode:
- CAGTTCAGCTACACACAGTAATTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AAMDC (28971)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465280
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28971 | AAMDC | adipogenesis associated Mth... | NM_001316960.1 | 69.6% | 63.1% | (many diffs) |
| 2 | human | 28971 | AAMDC | adipogenesis associated Mth... | NM_001316961.1 | 69.6% | 63.1% | (many diffs) |
| 3 | human | 28971 | AAMDC | adipogenesis associated Mth... | NM_001316962.1 | 69.6% | 63.1% | (many diffs) |
| 4 | human | 28971 | AAMDC | adipogenesis associated Mth... | NM_024684.3 | 69.6% | 63.1% | (many diffs) |
| 5 | human | 28971 | AAMDC | adipogenesis associated Mth... | XM_011544969.2 | 69.6% | 63.1% | (many diffs) |
| 6 | human | 28971 | AAMDC | adipogenesis associated Mth... | NM_001316958.1 | 60.5% | 56.8% | (many diffs) |
| 7 | human | 28971 | AAMDC | adipogenesis associated Mth... | NM_001363564.1 | 60.2% | 54.6% | (many diffs) |
| 8 | human | 28971 | AAMDC | adipogenesis associated Mth... | NM_001316957.1 | 56.4% | 53% | (many diffs) |
| 9 | human | 28971 | AAMDC | adipogenesis associated Mth... | XM_017017620.1 | 50% | 47% | (many diffs) |
| 10 | human | 28971 | AAMDC | adipogenesis associated Mth... | NR_133637.1 | 34.2% | (many diffs) | |
| 11 | mouse | 66273 | Aamdc | adipogenesis associated Mth... | NM_183251.3 | 62.8% | 57.3% | (many diffs) |
| 12 | mouse | 66273 | Aamdc | adipogenesis associated Mth... | XM_006508114.3 | 62.8% | 57.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 354
- ORF length:
- 264
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGACTTCCCC TGAAATTGCT TCCTTATCAT 121 GGGGGCAAAT GAAAGTAAAA GGCTCTAATA CAACCTATAA GGACTGCAAA GTATGGCCAG 181 GGGGTAGTCG GACTTGGGAT TGGAGAGAAA CAGGAACTGA GCATTCTCCT GGTGTGCAGC 241 CTGCAGATGT GAAGGAAGTT GTTGAGAAGG GTGTACAGAC TCTTGTGATT GGCCGAGGGA 301 TGAGTGAGGC CTTGAAGGAT GGAATACAGG GGGCACAGTT AGATCTGAAT TGTTACCCAA 361 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 421 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 481 TATCTTGTGG AAAGGACGAC AGTTCAGCTA CACACAGTAA TTTACGCGTT AAGTCgacaa 541 tcaacctctg gattacaaaa tttgtgaaag att