Construct: ORF TRCN0000465314
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013213.1_s317c1
- Derived from:
- ccsbBroadEn_12901
- DNA Barcode:
- AGCCAGTACTCTATTCCGAAGGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM86B1 (85002)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465314
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 348926 | FAM86EP | family with sequence simila... | NR_130742.1 | 7.3% | (many diffs) | |
| 2 | human | 645332 | FAM86C2P | family with sequence simila... | NR_024249.1 | 7.3% | (many diffs) | |
| 3 | human | 348926 | FAM86EP | family with sequence simila... | NR_130741.1 | 7.1% | (many diffs) | |
| 4 | human | 100125556 | FAM86JP | family with sequence simila... | NR_024250.1 | 7% | (many diffs) | |
| 5 | human | 729375 | FAM86HP | family with sequence simila... | NR_024252.1 | 6.9% | (many diffs) | |
| 6 | human | 85002 | FAM86B1 | family with sequence simila... | NR_003494.2 | 6.8% | 1_1292del;1443_2184del | |
| 7 | human | 653113 | FAM86FP | family with sequence simila... | NR_024254.1 | 6.8% | (many diffs) | |
| 8 | human | 85002 | FAM86B1 | family with sequence simila... | XR_001745614.2 | 6.7% | 1_1320del;1471_2208del | |
| 9 | human | 100125556 | FAM86JP | family with sequence simila... | NR_024251.1 | 6.7% | (many diffs) | |
| 10 | human | 85002 | FAM86B1 | family with sequence simila... | XR_001745612.2 | 6.4% | 1_1424del;1575_2312del | |
| 11 | human | 85002 | FAM86B1 | family with sequence simila... | XR_001745613.2 | 6.1% | 1_1566del;1717_2454del | |
| 12 | human | 692099 | FAM86DP | family with sequence simila... | NR_024241.1 | 5.7% | (many diffs) | |
| 13 | human | 348926 | FAM86EP | family with sequence simila... | NR_130740.1 | 4.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 183
- ORF end:
- 333
- ORF length:
- 150
- Sequence:
-
1 GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT 61 TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT 121 TCCATTTCAG GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG 181 GCATGAGGAG TACTGCAGAG GTCACAGGGA AGTCACAGAA CAGTAATACG CTAGCAGGGG 241 CATGGGGCAT GAAGAACAGA AGACAGGAAG CATTTCAGAG ACTCCAAAGA AGAAATCAGG 301 GCCAACCACA GCTTCCCGGG TCATTCACCA GGTGCCCAAC TTTCTTGTAC AAAGTGGTTG 361 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 421 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAG 481 CCAGTACTCT ATTCCGAAGG CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 541 ttgtgaaaga tt