Transcript: Human XR_001745612.2

PREDICTED: Homo sapiens family with sequence similarity 86 member B1 (FAM86B1), transcript variant X13, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FAM86B1 (85002)
Length:
2312
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001745612.2
NBCI Gene record:
FAM86B1 (85002)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001745612.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000444970 CTGAGCTGCTGCGGGATATTT pLKO_005 220 3UTR 100% 15.000 7.500 Y EEF2KMT n/a
2 TRCN0000447299 TCTGAGCTGCTGCGGGATATT pLKO_005 219 3UTR 100% 13.200 6.600 Y FAM86C1 n/a
3 TRCN0000128827 GCTTTCTCTCAGAACTCATCA pLKO.1 304 3UTR 100% 4.950 2.475 Y FAM86B1 n/a
4 TRCN0000129161 GTGCTTTCTCTCAGAACTCAT pLKO.1 302 3UTR 100% 4.950 2.475 Y FAM86B1 n/a
5 TRCN0000130496 GACCAGAAACTGTTTCCCTAT pLKO.1 754 3UTR 100% 4.050 2.025 Y FAM86B1 n/a
6 TRCN0000131177 GCCTTCATTAACAGACGTGCT pLKO.1 552 3UTR 100% 2.160 1.080 Y FAM86B1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001745612.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488399 TACCTGCGCGCCTAACAATCATGA pLX_317 37.3% 26.4% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_09791 pDONR223 100% 26.3% None (many diffs) n/a
3 ccsbBroad304_09791 pLX_304 0% 26.3% V5 (many diffs) n/a
4 TRCN0000466174 GGAGAGATGACCTTCCATCATCTG pLX_317 21.3% 26.3% V5 (many diffs) n/a
5 TRCN0000488297 ACTAGACGAAAAATGAGGCATGGA pLX_317 30.4% 26.3% V5 (not translated due to prior stop codon) (many diffs) n/a
6 ccsbBroadEn_03547 pDONR223 100% 15.8% None (many diffs) n/a
7 ccsbBroad304_03547 pLX_304 0% 15.8% V5 (many diffs) n/a
8 TRCN0000473048 AGTGCATTTCATCTCTGTAGCAAC pLX_317 92.4% 15.8% V5 (many diffs) n/a
9 ccsbBroadEn_12901 pDONR223 100% 6.4% None 1_1424del;1575_2312del n/a
10 ccsbBroad304_12901 pLX_304 0% 6.4% V5 1_1424del;1575_2312del n/a
11 TRCN0000465314 AGCCAGTACTCTATTCCGAAGGCA pLX_317 100% 6.4% V5 1_1424del;1575_2312del n/a
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