Construct: ORF TRCN0000465345
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015586.1_s317c1
- Derived from:
- ccsbBroadEn_05043
- DNA Barcode:
- ACAAAGGCTTGAAGTAACCCCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEKT4 (150483)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465345
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150483 | TEKT4 | tektin 4 | NM_144705.4 | 100% | 100% | |
| 2 | human | 150483 | TEKT4 | tektin 4 | XM_011510670.2 | 91.3% | 91.3% | 935_1057del |
| 3 | human | 150483 | TEKT4 | tektin 4 | XM_005263876.3 | 71.9% | 71.7% | (many diffs) |
| 4 | human | 150483 | TEKT4 | tektin 4 | NM_001286559.2 | 58.1% | 58.1% | 0_1ins546 |
| 5 | human | 100233156 | LOC100233156 | tektin 4 pseudogene | NR_037871.1 | 44.5% | (many diffs) | |
| 6 | human | 727764 | MAFIP | MAFF interacting protein (p... | NR_046439.2 | 42.5% | (many diffs) | |
| 7 | human | 727764 | MAFIP | MAFF interacting protein (p... | NR_046440.2 | 42.3% | (many diffs) | |
| 8 | human | 100233156 | LOC100233156 | tektin 4 pseudogene | NR_037872.1 | 29.8% | (many diffs) | |
| 9 | human | 100132288 | TEKT4P2 | tektin 4 pseudogene 2 | NR_038327.2 | 28.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1371
- ORF length:
- 1305
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcagacagtg ccgccctgcg agctgccctg caaagagtac gacgtggccc 121 gtaacacggg cgcctacacg tcctccggcc tggccaccgc cagcttccgc acctccaagt 181 acctgctgga ggagtggttc cagaactgct atgctcgcta ccaccaggcc ttcgccgacc 241 gcgaccagtc ggagcggcag cggcacgaga gccagcagct ggccacagag acccaggcgc 301 tggcgcagcg cacgcagcaa gactccacgc gcacagtggg cgagcgactg caggacacgc 361 acagctggaa gtcggagctg cagcgtgaga tggaggcgct ggctgcggag accaacttgc 421 tcctggccca gaagcaacgg ctggagcgcg ccctggacgc cacagaggtg cccttctcca 481 tcaccactga caacctgcag tgccgtgagc gccgcgagca ccccaacctc gtgcgcgacc 541 atgtggaaac ggagctgctg aaggaagccg agctcatccg gaacattcag gagctgctga 601 agagaaccat catgcaagca gtgagccaga tccgactgaa ccgggagcac aaggagacct 661 gcgagatgga ctggtcagac aagatggagg cctacaacat cgacgagacc tgcgggcgcc 721 accacagcca gagcaccgag gtgcaggctc atccgtactc caccaccttc caagagagcg 781 cctccacccc ggagacccgg gccaagttca cgcaggacaa tctgtgccgt gcccagcgcg 841 agcgcctggc ctcggccaac ctgcgggtgc tggtggactg catccttcgc gacacctccg 901 aggacctgcg gctccagtgc gacgccgtga acctggcctt cgggcgccgc tgtgaggagc 961 tggaggacgc gcggtacaag ctgcatcacc acctgcacaa gacactgcgg gaaaTCACAG 1021 ATCAGGAACA CAACGTGGCG GCACTGAAGC AGGCCATCAA GGACAAAGAG GCACCTCTGC 1081 ACGTAGCCCA GACCCGGCTG TACCTGCGCT CGCACCGGCC CAACATGGAG CTGTGCCGTG 1141 ACGCAGCCCA GTTCAGGCTG TTGAGTGAGG TGGAGGAGCT GAACATGTCC CTCACAGCAC 1201 TGCGAGAGAA GCTTCTAGAA GCGGAGCAGT CCCTGCGCAA CCTCGAGGAC ATCCACATGA 1261 GCCTGGAGAA GGACATTGCC GCCATGACCA ACAGTCTCTT CATCGACCGC CAGAAGTGCA 1321 TGGCCCATCG TACTCGCTAC CCCACCATCC TGCAGCTGGC TGGCTACCAG TGCCCAACTT 1381 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1441 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1501 CTTGTGGAAA GGACGAACAA AGGCTTGAAG TAACCCCAAC ACGCGTTAAG TCgacaatca 1561 acctctggat tacaaaattt gtgaaagatt