Transcript: Human NR_046439.2

Homo sapiens MAFF interacting protein (pseudogene) (MAFIP), transcript variant 1, non-coding RNA.

Source:
NCBI, updated 2018-05-12
Taxon:
Homo sapiens (human)
Gene:
MAFIP (727764)
Length:
2760
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_046439.2
NBCI Gene record:
MAFIP (727764)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_046439.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000159185 GAGCAACAAGAAACGAATAAA pLKO.1 121 3UTR 100% 15.000 7.500 Y TEKT4P2 n/a
2 TRCN0000159736 GCAACAAGAAACGAATAAATC pLKO.1 123 3UTR 100% 13.200 6.600 Y TEKT4P2 n/a
3 TRCN0000180024 CTTCTCCATCACCACTGACAA pLKO.1 728 3UTR 100% 4.950 2.475 Y TEKT4 n/a
4 TRCN0000161734 CCAAGAGCAACAAGAAACGAA pLKO.1 117 3UTR 100% 3.000 1.500 Y TEKT4P2 n/a
5 TRCN0000162856 CGGGAAATCACAGATCAGGAA pLKO.1 2199 3UTR 100% 2.640 1.320 Y TEKT4P2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_046439.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05043 pDONR223 100% 42.5% None (many diffs) n/a
2 ccsbBroad304_05043 pLX_304 0% 42.5% V5 (many diffs) n/a
3 TRCN0000465345 ACAAAGGCTTGAAGTAACCCCAAC pLX_317 27.6% 42.5% V5 (many diffs) n/a
4 ccsbBroadEn_05743 pDONR223 100% 13.2% None (many diffs) n/a
5 ccsbBroad304_05743 pLX_304 0% 13.2% V5 (many diffs) n/a
6 TRCN0000474564 GCGGCATACTTCGGGCCGAACAGT pLX_317 100% 13.2% V5 (many diffs) n/a
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