Construct: ORF TRCN0000465354
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016361.1_s317c1
- Derived from:
- ccsbBroadEn_03731
- DNA Barcode:
- TGCGAGTGCCCGGAAGGCCCCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLDND1 (56650)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465354
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 56650 | CLDND1 | claudin domain containing 1 | NM_001040181.2 | 100% | 100% | |
2 | human | 56650 | CLDND1 | claudin domain containing 1 | NM_001040183.1 | 100% | 100% | |
3 | human | 56650 | CLDND1 | claudin domain containing 1 | NM_001040199.1 | 100% | 100% | |
4 | human | 56650 | CLDND1 | claudin domain containing 1 | NM_019895.2 | 100% | 100% | |
5 | human | 56650 | CLDND1 | claudin domain containing 1 | NM_001040182.1 | 91.6% | 91.6% | 1_69del |
6 | human | 56650 | CLDND1 | claudin domain containing 1 | NM_001040200.1 | 62.1% | 61.6% | (many diffs) |
7 | mouse | 224250 | Cldnd1 | claudin domain containing 1 | NM_171826.3 | 85.5% | 91.6% | (many diffs) |
8 | mouse | 224250 | Cldnd1 | claudin domain containing 1 | XM_006522094.3 | 85.5% | 91.6% | (many diffs) |
9 | mouse | 224250 | Cldnd1 | claudin domain containing 1 | NM_001252450.1 | 78.4% | 84% | (many diffs) |
10 | mouse | 224250 | Cldnd1 | claudin domain containing 1 | XM_011245886.2 | 70.2% | 73.9% | (many diffs) |
11 | mouse | 224250 | Cldnd1 | claudin domain containing 1 | NM_001252451.1 | 64.4% | 67.7% | (many diffs) |
12 | mouse | 224250 | Cldnd1 | claudin domain containing 1 | NR_045517.1 | 30.3% | (many diffs) | |
13 | mouse | 224250 | Cldnd1 | claudin domain containing 1 | NR_045518.1 | 24.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 825
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga taaccgtttt gctacagcat ttgtaattgc ttgtgtgctt agcctcattt 121 ccaccatcta catggcagcc tccattggca cagacttctg gtatgaatat cgaagtccag 181 ttcaagaaaa ttccagtgat ttgaataaaa gcatctggga tgaattcatt agtgatgagg 241 cagatgaaaa gacttataat gatgcacttt ttcgatacaa tggcacagtg ggattgtgga 301 gacggtgtat caccataccc aaaaacatgc attggtatag cccaccagaa aggacagagt 361 catttgatgt ggtcacaaaa tgtgtgagtt tcacactaac tgagcagttc atggagaaat 421 ttgttgatcc cggaaaccac aatagcggga ttgatctcct taggacctat ctttGGCGTT 481 GCCAGTTCCT TTTACCTTTT GTGAGTTTAG GTTTGATGTG CTTTGGGGCT TTGATCGGAC 541 TTTGTGCTTG CATTTGCCGA AGCTTATATC CCACCATTGC CACGGGCATT CTCCATCTCC 601 TTGCAGGTCT GTGTACACTG GGCTCAGTAA GTTGTTATGT TGCTGGAATT GAACTACTCC 661 ACCAGAAACT AGAGCTCCCT GACAATGTAT CCGGTGAATT TGGATGGTCC TTCTGCCTGG 721 CTTGTGTCTC TGCTCCCTTA CAGTTCATGG CTTCTGCTCT CTTCATCTGG GCTGCTCACA 781 CCAACCGGAA AGAGTACACC TTAATGAAGG CATATCGTGT GGCATGCCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA TGCGAGTGCC CGGAAGGCCC CTTAACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt