Construct: ORF TRCN0000465420
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008522.1_s317c1
- Derived from:
- ccsbBroadEn_09117
- DNA Barcode:
- AATATCTCAGCGAGGGGATTAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYT16 (83851)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465420
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83851 | SYT16 | synaptotagmin 16 | NM_001367656.1 | 99.9% | 99.8% | 29T>C |
| 2 | human | 83851 | SYT16 | synaptotagmin 16 | NM_001367661.1 | 99.9% | 99.8% | 29T>C |
| 3 | human | 83851 | SYT16 | synaptotagmin 16 | NM_001367663.1 | 99.9% | 99.8% | 29T>C |
| 4 | human | 83851 | SYT16 | synaptotagmin 16 | XM_006720272.3 | 99.9% | 99.8% | 29T>C |
| 5 | human | 83851 | SYT16 | synaptotagmin 16 | XM_024449720.1 | 99.9% | 99.8% | 29T>C |
| 6 | human | 83851 | SYT16 | synaptotagmin 16 | XM_024449721.1 | 99.9% | 99.8% | 29T>C |
| 7 | human | 83851 | SYT16 | synaptotagmin 16 | XM_024449723.1 | 99.9% | 99.8% | 29T>C |
| 8 | human | 83851 | SYT16 | synaptotagmin 16 | XM_024449724.1 | 99.9% | 99.8% | 29T>C |
| 9 | human | 83851 | SYT16 | synaptotagmin 16 | XM_024449725.1 | 88% | 87.7% | (many diffs) |
| 10 | human | 83851 | SYT16 | synaptotagmin 16 | XM_024449726.1 | 84.9% | 84% | (many diffs) |
| 11 | human | 83851 | SYT16 | synaptotagmin 16 | XM_024449727.1 | 84% | 83.7% | (many diffs) |
| 12 | human | 83851 | SYT16 | synaptotagmin 16 | NM_001367652.1 | 78.2% | 71% | (many diffs) |
| 13 | human | 83851 | SYT16 | synaptotagmin 16 | NM_001367659.1 | 78.1% | 71.8% | (many diffs) |
| 14 | human | 83851 | SYT16 | synaptotagmin 16 | XM_011537227.2 | 75.8% | 72.9% | (many diffs) |
| 15 | human | 83851 | SYT16 | synaptotagmin 16 | NM_001367657.1 | 68.4% | 63.8% | (many diffs) |
| 16 | human | 83851 | SYT16 | synaptotagmin 16 | XR_002957566.1 | 68.1% | (many diffs) | |
| 17 | human | 83851 | SYT16 | synaptotagmin 16 | NM_001367654.1 | 67.3% | 62.4% | (many diffs) |
| 18 | human | 83851 | SYT16 | synaptotagmin 16 | XM_011537229.2 | 64.1% | 62.7% | (many diffs) |
| 19 | human | 83851 | SYT16 | synaptotagmin 16 | XM_017021697.2 | 62.6% | 55.6% | (many diffs) |
| 20 | human | 83851 | SYT16 | synaptotagmin 16 | NM_001367650.1 | 52.5% | 52.5% | 0_1ins918 |
| 21 | human | 83851 | SYT16 | synaptotagmin 16 | XM_024449728.1 | 52.5% | 52.5% | 0_1ins918 |
| 22 | human | 83851 | SYT16 | synaptotagmin 16 | XM_024449729.1 | 52.5% | 52.5% | 0_1ins918 |
| 23 | human | 83851 | SYT16 | synaptotagmin 16 | XM_024449730.1 | 52.5% | 52.5% | 0_1ins918 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2004
- ORF length:
- 1935
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtgttggcc atggcgtctc aggatgctca gaacttcttc cagcctttct 121 cttcctggat atctcgggtt tatgaagctc tccagcaagc aggagatatg ttatctgctt 181 cgctggttaa cataagcaaa caagactcta aattgagtga caaactagat caggacttag 241 ataatattca gattcaggaa acgtactttg aagatgaaga acaagacaat gattggagtc 301 aagaggatgc aaattccttg tttcttgaag tggatcattt ctcatgttgt aatagtgatt 361 tgcaggactc tgcccaaaat tcaagcccaa gccttagcca acatgcaaag gactcatgtt 421 ccacaatgtc ccagtggccc aattgggcca gtgatgaccg caagttacca catgtgcttt 481 cttctattgc ggaggaagag catcaccttg aaaagcaaag aagtggcctt caacatggct 541 ttgacagcca gctccctggt actttagaaa ctgttaatgg aaaaaagcaa gtcaacagct 601 ttggggatga cgaagagctg tccacatctt ctgacagtga cgaggaggtg atcaaacaat 661 ttgagatttc cgtgtcccgg tcccagagtt tccgttcagt gacatctgag aaaggaaagc 721 agacaggatt ggagcagaaa ccaaaattca gccgttcgtt gttgacacac ggagaagatg 781 gcacagaagt atctgcctgc gaagatttgg atggagccag ccaacggcgt tattctgaga 841 atctctccta cggtgaagat gaccacatcc ctgctcactc acagtcccca tgtgaaagag 901 gggatgccaa acaccacggc acatctcacc aagagtccag tgtggtccaa agcctcaggc 961 gccaatccac agagggcagc ttggagatgg agacagcttt taatagccgg ggatttgaag 1021 attcctatgc cactgacagc tcctccatgt ggagtccaga ggaacaggac aggaccaatt 1081 tgcaggtgcc atccggggtc tcagagccca tctcaaagtg tggtgaccta gatgtcatct 1141 ttgaatatag agccgccagc cagaagctca cagtgaccat tgtgagggca cagggcctcc 1201 cagataagga ccgaagtggt gtcaactcct ggcaagttca tgtagtgctg ctgcctggta 1261 agaaacacag gggcaggacg aacatacaga gagggcccaa ccccgtcttc agggagaagg 1321 tcacctttgc caagctggag cccagagatg tggctgcctg tgctgtccgc ttccgcctgt 1381 acgctgcccg gaagatgacc cgagagagaa tgatgggaga gaaactattc tatctcagcc 1441 acctgcaccc agaaggggaa atgaaagtga ctctggttct ggagccaaga agtaatataa 1501 gcagtggagg gtctccgctc agcccatctg cggtttctca cagtgatagt acttcatcca 1561 cgcagtcgct gtctcatgga ggggcgccag agctgttggt ggggctctcg tacaatgcca 1621 caacggggcg attatctgtg gaaatgatca aaggcagcca tttccgaaac ctcgctgtta 1681 accgagcacc TGATACATAT GGAAAACTCT TTCTCCTCAA TTCTGTGGGT CAAGAGATGT 1741 CCCGTTGCAA GACGTCCATT CGGCGTGGTC AGCCCAATCC TGTCTATAAG GAGACCTTTG 1801 TTTTCCAGGT GGCCCTCTTT CAGCTGTCTG ATGTCACGTT GATGATTTCC GTTTATAACA 1861 GGCGTACTAT GAAGCGTAAA GAGATGATTG GCTGGATTGC CCTGGGCCAG AACAGCAGTG 1921 GAGAGGAGGA ACAAGATCAC TGGGAGGAGA TGAAGGAAAC CAAAGGCCAG CAGATCTGCA 1981 GATGGCACAC TTTGCTGGAA TCCTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 2041 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2101 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ATATCTCAGC 2161 GAGGGGATTA ATAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2221 att