Transcript: Human XM_024449728.1

PREDICTED: Homo sapiens synaptotagmin 16 (SYT16), transcript variant X19, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SYT16 (83851)
Length:
13848
CDS:
986..2005

Additional Resources:

NCBI RefSeq record:
XM_024449728.1
NBCI Gene record:
SYT16 (83851)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024449728.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000314674 TTTCCGAAACCTCGCTGTTAA pLKO_005 1660 CDS 100% 13.200 18.480 N SYT16 n/a
2 TRCN0000314745 AGGCGTACTATGAAGCGTAAA pLKO_005 1859 CDS 100% 10.800 15.120 N SYT16 n/a
3 TRCN0000001760 CGTTGATGATTTCCGTTTATA pLKO.1 1836 CDS 100% 15.000 12.000 N SYT16 n/a
4 TRCN0000314744 GTTGATGATTTCCGTTTATAA pLKO_005 1837 CDS 100% 15.000 10.500 N SYT16 n/a
5 TRCN0000314742 GTTACTATTTGCAACCATTTA pLKO_005 2369 3UTR 100% 13.200 9.240 N SYT16 n/a
6 TRCN0000001757 CAGACAGGATTGGAGCAGAAA pLKO.1 719 5UTR 100% 4.950 3.465 N SYT16 n/a
7 TRCN0000001759 GAGAGAATGATGGGAGAGAAA pLKO.1 1403 CDS 100% 4.950 3.465 N SYT16 n/a
8 TRCN0000314741 GAGAGAATGATGGGAGAGAAA pLKO_005 1403 CDS 100% 4.950 3.465 N SYT16 n/a
9 TRCN0000001758 GAGGAGATGAAGGAAACCAAA pLKO.1 1943 CDS 100% 4.950 3.465 N SYT16 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024449728.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12755 pDONR223 100% 59.8% 59.8% None 1_408del n/a
2 ccsbBroad304_12755 pLX_304 0% 59.8% 59.8% V5 1_408del n/a
3 TRCN0000479179 GACCTTACCGCCTCTGTGCTATGT pLX_317 77.2% 59.8% 59.8% V5 1_408del n/a
4 ccsbBroadEn_09117 pDONR223 100% 52.5% 52.5% None 0_1ins918 n/a
5 ccsbBroad304_09117 pLX_304 0% 52.5% 52.5% V5 0_1ins918 n/a
6 TRCN0000465420 AATATCTCAGCGAGGGGATTAATA pLX_317 16.5% 52.5% 52.5% V5 0_1ins918 n/a
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