Transcript: Human NM_001367654.1

Homo sapiens synaptotagmin 16 (SYT16), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-08-07
Taxon:
Homo sapiens (human)
Gene:
SYT16 (83851)
Length:
13333
CDS:
96..1490

Additional Resources:

NCBI RefSeq record:
NM_001367654.1
NBCI Gene record:
SYT16 (83851)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001367654.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000314674 TTTCCGAAACCTCGCTGTTAA pLKO_005 1145 CDS 100% 13.200 18.480 N SYT16 n/a
2 TRCN0000314745 AGGCGTACTATGAAGCGTAAA pLKO_005 1344 CDS 100% 10.800 15.120 N SYT16 n/a
3 TRCN0000001760 CGTTGATGATTTCCGTTTATA pLKO.1 1321 CDS 100% 15.000 12.000 N SYT16 n/a
4 TRCN0000314744 GTTGATGATTTCCGTTTATAA pLKO_005 1322 CDS 100% 15.000 10.500 N SYT16 n/a
5 TRCN0000314742 GTTACTATTTGCAACCATTTA pLKO_005 1854 3UTR 100% 13.200 9.240 N SYT16 n/a
6 TRCN0000001759 GAGAGAATGATGGGAGAGAAA pLKO.1 888 CDS 100% 4.950 3.465 N SYT16 n/a
7 TRCN0000314741 GAGAGAATGATGGGAGAGAAA pLKO_005 888 CDS 100% 4.950 3.465 N SYT16 n/a
8 TRCN0000001758 GAGGAGATGAAGGAAACCAAA pLKO.1 1428 CDS 100% 4.950 3.465 N SYT16 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001367654.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09117 pDONR223 100% 67.3% 62.4% None (many diffs) n/a
2 ccsbBroad304_09117 pLX_304 0% 67.3% 62.4% V5 (many diffs) n/a
3 TRCN0000465420 AATATCTCAGCGAGGGGATTAATA pLX_317 16.5% 67.3% 62.4% V5 (many diffs) n/a
4 ccsbBroadEn_12755 pDONR223 100% 43.7% 43.7% None 1_783del n/a
5 ccsbBroad304_12755 pLX_304 0% 43.7% 43.7% V5 1_783del n/a
6 TRCN0000479179 GACCTTACCGCCTCTGTGCTATGT pLX_317 77.2% 43.7% 43.7% V5 1_783del n/a
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