Construct: ORF TRCN0000465430
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013410.1_s317c1
- Derived from:
- ccsbBroadEn_13433
- DNA Barcode:
- GCGACATGGAAGAGCTAACTACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIAA1324L (222223)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465430
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 222223 | KIAA1324L | KIAA1324 like | NM_001291991.1 | 97.9% | 97.5% | (many diffs) |
| 2 | human | 222223 | KIAA1324L | KIAA1324 like | NM_152748.4 | 93.1% | 93.1% | 1_177del |
| 3 | human | 222223 | KIAA1324L | KIAA1324 like | XM_017011844.1 | 93.1% | 93.1% | 1_177del |
| 4 | human | 222223 | KIAA1324L | KIAA1324 like | NM_001291990.1 | 87.7% | 87.7% | 1_336del |
| 5 | human | 222223 | KIAA1324L | KIAA1324 like | XM_006715894.3 | 87.7% | 87.7% | 1_336del |
| 6 | human | 222223 | KIAA1324L | KIAA1324 like | XM_011515921.1 | 87.7% | 87.7% | 1_336del |
| 7 | human | 222223 | KIAA1324L | KIAA1324 like | XM_011515918.2 | 82.4% | 82.4% | 1_513del |
| 8 | human | 222223 | KIAA1324L | KIAA1324 like | NM_001142749.3 | 78% | 78% | 1_678del |
| 9 | human | 222223 | KIAA1324L | KIAA1324 like | XM_017011843.1 | 71.8% | 71% | 1_678del;2864_2865ins166;2895_2896ins26 |
| 10 | human | 222223 | KIAA1324L | KIAA1324 like | XM_024446686.1 | 65.7% | 65.7% | 0_1ins825 |
| 11 | mouse | 231014 | 9330182L06Rik | RIKEN cDNA 9330182L06 gene | XM_006503570.1 | 78.1% | 83% | (many diffs) |
| 12 | mouse | 231014 | 9330182L06Rik | RIKEN cDNA 9330182L06 gene | XM_006503571.3 | 78.1% | 83% | (many diffs) |
| 13 | mouse | 231014 | 9330182L06Rik | RIKEN cDNA 9330182L06 gene | NM_172706.3 | 69.5% | 73.9% | (many diffs) |
| 14 | mouse | 231014 | 9330182L06Rik | RIKEN cDNA 9330182L06 gene | XR_376692.3 | 36.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2478
- ORF length:
- 2409
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggacaccacc actgacaagt gggtaaaact tacagacaat ggagaatggg 121 gctctcattc tgtaatgctg aaatcaggca caaacatact ctactggaga actacaggca 181 tccttatggg ttctaaggcg gtcaagcctg tgctggtaaa aaatatcaca attgaagggg 241 tggcgtacac atcagaatgt tttccttgca agccaggcac attcagcaac aaaccaggtt 301 cattcaactg ccaggtgtgt cccagaaaca cctattctga gaaaggagcc aaagaatgta 361 taaggtgtaa agacgactct caattttcag aggaaggatc cagtgagtgt acagagcgcc 421 ctccctgtac cacaaaagac tatttccaga tccatactcc atgtgatgaa gaaggaaaga 481 cacagataat gtacaagtgg atagagccca aaatctgccg ggaggatctc acagatgcta 541 ttagattgcc cccttctgga gagaagaagg attgtccgcc ttgcaaccct ggattttata 601 acaatggatc atcttcttgc catccctgtc ctcctggaac attttcagat ggaaccaaag 661 aatgtagacc atgtccagca ggaacggagc ctgcacttgg ctttgaatat aaatggtgga 721 atgtccttcc tggcaacatg aaaacttcct gcttcaatgt tgggaattca aagtgcgatg 781 gaatgaatgg ttgggaggtg gctggagatc atatccagag tggggctgga ggttctgaca 841 atgattacct gatcttaaac ttgcatatcc caggatttaa accaccaaca tctatgactg 901 gagccacggg ttctgaacta ggaagaataa catttgtctt tgagaccctc tgttcagctg 961 actgtgtttt gtacttcatg gtggatatta atagaaaaag tacaaatgtg gtagaatcgt 1021 ggggtggaac caaagaaaaa caagcttaca cccatatcat cttcaagaat gcaactttta 1081 catttacatg ggcattccag agaactaatc agggtcaaga taatagacgg ttcatcaatg 1141 acatggtgaa gatttattct atcacagcca ctaatgcagt tgatggggtg gcgtcctcat 1201 gccgtgcctg tgccctcggt tctgaacagt cgggttcatc gtgtgtcccc tgccctccag 1261 gccactacat tgagaaagaa accaaccagt gcaaggaatg tccacctgac acctacctgt 1321 ccatacatca ggtctatggc aaagaggctt gtattccatg cgggcctggg agtaaaaaca 1381 atcaggacca ttcggtttgc tatagtgact gctttttcta ccatgaaaaa gaaaatcaga 1441 gtttgcacta tgactttagc aacctcagca gtgtgggctc attaatgaat ggccccagct 1501 tcacctccaa aggaacaaaa tacttccatt tcttcaatat cagtttatgt gggcatgagg 1561 ggaagaagat ggctctctgt accaacaata taacagactt tacagtaaaa gaaatagtgg 1621 cagggtcaga tgattacaca aatttggtag gggcatttgt atgccagtca acaattattc 1681 cttctgaaag taagggtttc cgagcagcct tatcatcaca atccatcatt ctggcagata 1741 cattcatagg agtcacagtt gaaaccacat tgaaaaatat taatataaaa gaagatatgt 1801 tcccagttcc aacaagccaa ataccagatg tgcatttctt ttataagtct tctacagcaa 1861 caacatcttg tattaatggc cgatcaactg ctgtgaaaat gaggtgtaat cctactaaat 1921 ctggagcagg agtgatttca gtccccagca agtgcccagc aggtacctgt gatgggtgta 1981 cgttctattt cctgtgggag agtgctgaag cttgccctct gtgtacggag catgacttcc 2041 atgagattga gggagcctgc aagagaggat ttcaggaaac cttgtatgtg tggaatgaac 2101 ctaaatggtg cattaaagga atttctttgc cTGAGAAAAA GTTGGCAACC TGTGAAACGG 2161 TTGACTTTTG GCTGAAGGTG GGAGCCGGTG TGGGAGCTTT TACTGCCGTT TTGCTGGTGG 2221 CTCTGACCTG CTACTTCTGG AAAAAGAATC AAAAACTGGA ATACAAATAT TCCAAGTTAG 2281 TAATGACGAC TAACTCAAAA GAGTGTGAAC TCCCGGCTGC AGACAGTTGT GCTATCATGG 2341 AAGGAGAAGA TAATGAAGAG GAAGTTGTAT ATTCCAATAA ACAGTCACTA CTAGGAAAAC 2401 TCAAATCTTT GGCAACCAAG GAAAAAGAAG ACCATTTTGA ATCTGTTCAA CTGAAAACCT 2461 CAAGATCCCC AAATATATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2521 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2581 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCGACAT GGAAGAGCTA 2641 ACTACATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt