Construct: ORF TRCN0000465452
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001434.1_s317c1
- Derived from:
- ccsbBroadEn_01283
- DNA Barcode:
- ACTCTCTGTTATTACCTATAAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKCE (5581)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465452
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5581 | PRKCE | protein kinase C epsilon | NM_005400.3 | 100% | 100% | |
| 2 | human | 5581 | PRKCE | protein kinase C epsilon | XM_005264428.1 | 100% | 100% | |
| 3 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532971.3 | 86% | 84.1% | (many diffs) |
| 4 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532975.3 | 86% | 84.1% | (many diffs) |
| 5 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004486.2 | 86% | 84.1% | (many diffs) |
| 6 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532978.2 | 84.3% | 84.2% | 1_1delAins345;4_5delCA |
| 7 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004487.2 | 84.3% | 84.2% | 1_1delAins345;4_5delCA |
| 8 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004488.2 | 84.3% | 84.2% | 1_1delAins345;4_5delCA |
| 9 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532980.3 | 79.6% | 79.6% | 0_1ins450 |
| 10 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532981.3 | 79.6% | 79.6% | 0_1ins450 |
| 11 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004489.2 | 79.6% | 79.6% | 0_1ins450 |
| 12 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004490.2 | 79.6% | 79.6% | 0_1ins450 |
| 13 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004491.2 | 79.6% | 79.6% | 0_1ins450 |
| 14 | human | 5581 | PRKCE | protein kinase C epsilon | XM_024452991.1 | 79.6% | 79.6% | 0_1ins450 |
| 15 | human | 5581 | PRKCE | protein kinase C epsilon | XM_024452992.1 | 79.6% | 79.6% | 0_1ins450 |
| 16 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532982.2 | 67.4% | 67.4% | 0_1ins720 |
| 17 | human | 5581 | PRKCE | protein kinase C epsilon | XM_005264431.4 | 65.9% | 65.2% | (many diffs) |
| 18 | human | 5581 | PRKCE | protein kinase C epsilon | XM_017004492.2 | 65.1% | 64.9% | (many diffs) |
| 19 | human | 5581 | PRKCE | protein kinase C epsilon | XM_006712050.3 | 62.4% | 62.4% | 0_1ins831 |
| 20 | human | 5581 | PRKCE | protein kinase C epsilon | XM_011532983.2 | 58.2% | 57.2% | (many diffs) |
| 21 | human | 5581 | PRKCE | protein kinase C epsilon | XR_939695.2 | 54.7% | (many diffs) | |
| 22 | human | 5581 | PRKCE | protein kinase C epsilon | XR_001738846.2 | 18% | (many diffs) | |
| 23 | mouse | 18754 | Prkce | protein kinase C, epsilon | NM_011104.3 | 91% | 97.9% | (many diffs) |
| 24 | mouse | 18754 | Prkce | protein kinase C, epsilon | XM_017317328.1 | 85.6% | 91.7% | (many diffs) |
| 25 | mouse | 18754 | Prkce | protein kinase C, epsilon | XM_006523833.2 | 74.3% | 79.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2280
- ORF length:
- 2211
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtagtgttc aatggccttc ttaagatcaa aatctgcgag gccgtgagct 121 tgaagcccac agcctggtcg ctgcgccatg cggtgggacc ccggccgcag actttccttc 181 tcgaccccta cattgccctc aatgtggacg actcgcgcat cggccaaacg gccaccaagc 241 agaagaccaa cagcccggcc tggcacgacg agttcgtcac cgatgtgtgc aacggacgca 301 agatcgagct ggctgtcttt cacgatgccc ccataggcta cgacgacttc gtggccaact 361 gcaccatcca gtttgaggag ctgctgcaga acgggagccg ccacttcgag gactggattg 421 atctggagcc agaaggaaga gtgtatgtga tcatcgatct ctcagggtcg tcgggtgaag 481 cccctaaaga caatgaagag cgtgtgttca gggaacgcat gcggccgagg aagcggcagg 541 gggccgtcag gcgcagggtc catcaggtca acggccacaa gttcatggcc acctatcttc 601 ggcagcccac ctactgctcc cattgcagag acttcatctg gggtgtcata ggaaagcagg 661 gataccagtg tcaagtctgc acctgcgtgg tccacaagcg gtgccacgag ctcataatca 721 caaagtgtgc tgggttaaag aagcaggaga cccccgacca ggtgggctcc cagcggttca 781 gcgtcaacat gccccacaag ttcggtatcc acaactacaa ggtccctacc ttctgcgatc 841 actgtgggtc cctgctctgg ggactcttgc ggcagggttt gcagtgtaaa gtctgcaaaa 901 tgaatgttca ccgtcgatgt gagaccaacg tggctcccaa ctgtggagtg gatgccagag 961 gaatcgccaa agtactggcc gacctgggcg ttaccccaga caaaatcacc aacagcggcc 1021 agagaaggaa aaagctcatt gctggtgccg agtccccgca gcctgcttct ggaagctcac 1081 catctgagga agatcgatcc aagtcagcac ccacctcccc ttgtgaccag gaaataaaag 1141 aacttgagaa caacattcgg aaagccttgt catttgacaa ccgaggagag gagcaccggg 1201 cagcatcgtc tcctgatggc cagctgatga gccccggtga gaatggcgaa gtccggcaag 1261 gccaggccaa gcgcctgggc ctggatgagt tcaacttcat caaggtgttg ggcaaaggca 1321 gctttggcaa ggtcatgttg gcagaactca agggcaaaga tgaagtatat gctgtgaagg 1381 tcttaaagaa ggacgtcatc cttcaggatg atgacgtgga ctgcacaatg acagagaaga 1441 ggattttggc tctggcacgg aaacacccgt accttaccca actctactgc tgcttccaga 1501 ccaaggaccg cctctttttc gtcatggaat atgtaaatgg tggagacctc atgtttcaga 1561 ttcagcgctc ccgaaaattc gacgagcctc gttcacggtt ctatgctgca gaggtcacat 1621 cggccctcat gttcctccac cagcatggag tcatctacag ggatttgaaa ctggacaaca 1681 tccttctgga tgcagaaggt cactgcaagc tggctgactt cgggatgtgc aaggaaggga 1741 ttctgaatgg tgtgacgacc accacgttct gtgggactcc tgactacata gctcctgaga 1801 tcctgcagga gttggagtat ggcccctccg tggactggtg ggccctgggg gtgctgatgt 1861 acgagatgat ggctggacag cctccctttg aggccgacaa tgaggacgac ctatttgagt 1921 ccatccTCCA TGACGACGTG CTGTACCCAG TCTGGCTCAG CAAGGAGGCT GTCAGCATCT 1981 TGAAAGCTTT CATGACGAAG AATCCCCACA AGCGCCTGGG CTGTGTGGCA TCGCAGAATG 2041 GCGAGGACGC CATCAAGCAG CACCCATTCT TCAAAGAGAT TGACTGGGTG CTCCTGGAGC 2101 AGAAGAAGAT CAAGCCACCC TTCAAACCAC GCATTAAAAC CAAAAGAGAC GTCAATAATT 2161 TTGACCAAGA CTTTACCCGG GAAGAGCCGG TACTCACCCT TGTGGACGAA GCAATTGTAA 2221 AGCAGATCAA CCAGGAGGAA TTCAAAGGTT TCTCCTACTT TGGTGAAGAC CTGATGCCCT 2281 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2341 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2401 TTTATATATC TTGTGGAAAG GACGAACTCT CTGTTATTAC CTATAAACGA CGCGTTAAGT 2461 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt