Construct: ORF TRCN0000465493
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014272.1_s317c1
- Derived from:
- ccsbBroadEn_12087
- DNA Barcode:
- CTCGCACGTAGCCTCCAGTGACCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AFTPH (54812)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465493
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54812 | AFTPH | aftiphilin | NM_001002243.3 | 15.1% | 15% | 1_2253del;2597A>G;2668_2724del |
| 2 | human | 54812 | AFTPH | aftiphilin | NM_017657.5 | 15.1% | 15% | (many diffs) |
| 3 | human | 54812 | AFTPH | aftiphilin | NM_203437.3 | 14.7% | 14.6% | (many diffs) |
| 4 | human | 54812 | AFTPH | aftiphilin | XM_005264380.2 | 14.6% | 14.6% | (many diffs) |
| 5 | human | 54812 | AFTPH | aftiphilin | XR_244941.4 | 8.7% | (many diffs) | |
| 6 | human | 54812 | AFTPH | aftiphilin | XR_244940.4 | 8.7% | (many diffs) | |
| 7 | mouse | 216549 | Aftph | aftiphilin | NM_181411.4 | 13.7% | 13.8% | (many diffs) |
| 8 | mouse | 216549 | Aftph | aftiphilin | NM_001290545.1 | 13.3% | 13.4% | (many diffs) |
| 9 | mouse | 216549 | Aftph | aftiphilin | XM_006514625.3 | 12.8% | 13% | (many diffs) |
| 10 | mouse | 216549 | Aftph | aftiphilin | NM_001252503.2 | 7.6% | 7.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 480
- ORF length:
- 414
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta tgcagcagga ttgggtatgt tagagcccac caaggaacca ctgaaaccac 121 tttctgctgc agaaaaaata gcttccatcg gtcagacagc caccatgtca ccagatatga 181 acacatgtac atctgatcag ttccaggagt ctctaccacc cgtccagttt gactggagta 241 gcagtggcct tactaaccct ttagatggtg tggatCCGGA GTTGTATGAG TTAACAACTT 301 CTAAGCTGGA AATCTCCACC TCAAGCCTCA AAGTGACTGA TGCATTTGCA AGACTCATGT 361 CTACAGTAGA GAAGACAAGC ACATCTACCA GGAAACCGAA AAGAGAAGGG CACCTAAGTG 421 AAGAAGCTAT CAAGGTGATC GCTGGCCTTC CTGACTTAAC ATTCATGCAT GCCAAGGTGT 481 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 541 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 601 TTTATATATC TTGTGGAAAG GACGACTCGC ACGTAGCCTC CAGTGACCGA CGCGTTAAGT 661 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt