Construct: ORF TRCN0000465501

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002965.1_s317c1
Derived from:
ccsbBroadEn_05939
DNA Barcode:
CTGAATGTTGTTTTAGAGTTACGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CBLB (868)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465501
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 868 CBLB Cbl proto-oncogene B NM_001321788.2 99.8% 99.8% (many diffs)
2 human 868 CBLB Cbl proto-oncogene B NM_170662.5 99.8% 99.8% (many diffs)
3 human 868 CBLB Cbl proto-oncogene B XM_011513257.1 99.8% 99.8% (many diffs)
4 human 868 CBLB Cbl proto-oncogene B XM_017007395.1 97.6% 97.7% (many diffs)
5 human 868 CBLB Cbl proto-oncogene B NM_001321786.1 97% 97.1% (many diffs)
6 human 868 CBLB Cbl proto-oncogene B NM_001321791.2 95.3% 95.4% (many diffs)
7 human 868 CBLB Cbl proto-oncogene B NM_001321793.2 95.3% 95.4% (many diffs)
8 human 868 CBLB Cbl proto-oncogene B NM_001321794.2 94.8% 94.9% (many diffs)
9 human 868 CBLB Cbl proto-oncogene B NM_001321795.2 94.8% 94.9% (many diffs)
10 human 868 CBLB Cbl proto-oncogene B NM_001321796.2 94.8% 94.9% (many diffs)
11 human 868 CBLB Cbl proto-oncogene B XM_017007399.1 94.8% 94.9% (many diffs)
12 human 868 CBLB Cbl proto-oncogene B NM_001321790.2 93.2% 93.3% (many diffs)
13 human 868 CBLB Cbl proto-oncogene B XM_017007398.1 92.7% 92.8% (many diffs)
14 human 868 CBLB Cbl proto-oncogene B XM_017007396.1 92.7% 92.7% (many diffs)
15 human 868 CBLB Cbl proto-oncogene B NM_001321789.1 92.2% 92.2% (many diffs)
16 human 868 CBLB Cbl proto-oncogene B XM_017007397.1 92.2% 92.2% (many diffs)
17 human 868 CBLB Cbl proto-oncogene B NM_001321797.2 90.3% 90.4% (many diffs)
18 human 868 CBLB Cbl proto-oncogene B NM_001321798.2 90.3% 90.4% (many diffs)
19 human 868 CBLB Cbl proto-oncogene B NM_001321799.2 90.3% 90.4% (many diffs)
20 human 868 CBLB Cbl proto-oncogene B XM_017007400.1 87.8% 87.9% (many diffs)
21 human 868 CBLB Cbl proto-oncogene B XM_011513259.3 85.3% 84.5% (many diffs)
22 human 868 CBLB Cbl proto-oncogene B NM_001321806.2 73.3% 73.4% (many diffs)
23 human 868 CBLB Cbl proto-oncogene B NM_001321807.2 73.3% 73.4% (many diffs)
24 human 868 CBLB Cbl proto-oncogene B NM_001321808.2 68.9% 68.9% (many diffs)
25 human 868 CBLB Cbl proto-oncogene B NM_001321811.2 68.3% 68.4% (many diffs)
26 human 868 CBLB Cbl proto-oncogene B NM_001321813.1 68.3% 68.4% (many diffs)
27 human 868 CBLB Cbl proto-oncogene B NM_001321816.2 63.9% 63.9% (many diffs)
28 human 868 CBLB Cbl proto-oncogene B NM_001321822.2 54.7% 54.7% (many diffs)
29 human 868 CBLB Cbl proto-oncogene B NM_001321820.2 54.6% 54.5% (many diffs)
30 human 868 CBLB Cbl proto-oncogene B NR_135811.2 44.6% (many diffs)
31 human 868 CBLB Cbl proto-oncogene B NR_135810.2 44% (many diffs)
32 human 868 CBLB Cbl proto-oncogene B XR_001740339.1 43.5% (many diffs)
33 human 868 CBLB Cbl proto-oncogene B XR_001740338.1 43.5% (many diffs)
34 human 868 CBLB Cbl proto-oncogene B NR_135808.2 43.4% (many diffs)
35 human 868 CBLB Cbl proto-oncogene B NR_135807.2 43.1% (many diffs)
36 human 868 CBLB Cbl proto-oncogene B NR_135812.1 42.4% (many diffs)
37 human 868 CBLB Cbl proto-oncogene B NR_135806.2 42.1% (many diffs)
38 human 868 CBLB Cbl proto-oncogene B NR_135809.2 40.9% (many diffs)
39 mouse 208650 Cblb Casitas B-lineage lymphoma b XM_006521947.1 88% 95% (many diffs)
40 mouse 208650 Cblb Casitas B-lineage lymphoma b XM_006521948.1 88% 95% (many diffs)
41 mouse 208650 Cblb Casitas B-lineage lymphoma b XM_006521949.1 88% 95% (many diffs)
42 mouse 208650 Cblb Casitas B-lineage lymphoma b XM_006521950.2 88% 95% (many diffs)
43 mouse 208650 Cblb Casitas B-lineage lymphoma b NM_001033238.1 84.2% 91.1% (many diffs)
44 mouse 208650 Cblb Casitas B-lineage lymphoma b XM_006521951.1 84.2% 91.1% (many diffs)
45 mouse 208650 Cblb Casitas B-lineage lymphoma b XM_006521952.3 74.1% 79.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3012
ORF length:
2946
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc aaactcaatg aatggcagaa accctggtgg tcgaggagga aatccccgaa
121aaggtcgaat tttgggtatt attgatgcta ttcaggatgc agttggaccc cctaagcaag
181ctgccgcaga tcgcaggacc gtggagaaga cttggaagct catggacaaa gtggtaagac
241tgtgccaaaa tcccaaactt cagttgaaaa atagcccacc atatatactt gatattttgc
301ctgatacata tcagcattta cgacttatat tgagtaaata tgatgacaac cagaaacttg
361cccaactcag tgagaatgag tactttaaaa tctacattga tagccttatg aaaaagtcaa
421aacgggcaat aagactcttt aaagaaggca aggagagaat gtatgaagaa cagtcacagg
481acagacgaaa tctcacaaaa ctgtccctta tcttcagtca catgctggca gaaatcaaag
541caatctttcc caatggtcaa ttccagggag ataactttcg tatcacaaaa gcagatgctg
601ctgaattctg gagaaagttt tttggagaca aaactatcgt accatggaaa gtattcagac
661agtgccttca tgaggtccac cagattagct ctggcctgga agcaatggct ctaaaatcaa
721caattgattt aacttgcaat gattacattt cagtttttga atttgatatt tttaccaggc
781tgtttcagcc ttggggctct attttgcgga attggaattt cttagctgtg acacatccag
841gttacatggc atttctcaca tatgatgaag ttaaagcacg actacagaaa tatagcacca
901aacccggaag ctatattttc cggttaagtt gcactcgatt gggacagtgg gccattggct
961atgtgactgg ggatgggaat atcttacaga ccatacctca taacaagccc ttatttcaag
1021ccctgattga tggcagcagg gaaggatttt atctttatcc tgatgggagg agttataatc
1081ctgatttaac tggattatgt gaacctacac ctcatgacca tataaaagtt acacaggaac
1141aatatgaatt atattgtgaa atgggctcca cttttcagct ctgtaagatt tgtgcagaga
1201atgacaaaga tgtcaagatt gagccttgtg ggcatttgat gtgcacctct tgccttacgg
1261catggcagga gtcggatggt cagggctgcc ctttctgtcg ttgtgaaata aaaggaactg
1321agcccataat cgtggatccc tttgatccaa gagatgaagg ctccaggtgt tgcagcatca
1381ttgacccctt tggcatgccg atgctcgact tggacgacga tgatgatcgt gaggagtcct
1441tgatgatgaa tcggttggca aacgtccgaa agtgcactga caggcagaac tcaccagtca
1501catcaccagg atcctctccc cttgcccaga gaagaaagcc acagcctgac ccactccaga
1561tcccacatct aagcctgcca cccgtgcctc ctcgcctgga tctaattcag aaaggcatag
1621ttagatctcc ctgtggcagc ccaacgggtt caccaaagtc ttctccttgc atggtgagaa
1681aacaagataa accactccca gcaccacctc ctcccttaag agatcctcct ccaccgccac
1741ctgaaagacc tccaccaatc ccaccagaca atagactgag tagacacatc catcatgtgg
1801aaagcgtgcc ttccaaagac ccgccaatgc ctcttgaagc atggtgccct cgggatgtgt
1861ttgggactaa tcagcttgtg ggatgtcgac tcctagggga gggctctcca aaacctggaa
1921tcacagcgag ttcaaatgtc aatggaaggc acagtagagt gggctctgac ccagtgctta
1981tgcggaaaca cagacgccat gatttgcctt tagaaggagc taaggtcttt tccaatggtc
2041accttggaag tgaagaatat gatgttcctc cccggctttc tcctcctcct ccagttacca
2101ccctcctccc tagcataaag tgtactggtc cgttagcaaa ttctctttca gagaaaacaa
2161gagacccagt agaggaagat gatgatgaat acaagattcc ttcatcccac cctgtttccc
2221tgaattcaca accatctcat tgtcataatg taaaacctcc tgttcggtct tgtgataatg
2281gtcactgtat gctgaatgga acacatggtc catcttcaga gaagaaatca aacatccctg
2341acttaagcat atatttaaag ggagatgttt ttgattcagc ctctgatccc gtgccattac
2401cacctgccag gcctccaact cgggacaatc caaagcatgg ttcttcactc aacaggacgc
2461cctctgatta tgatcttctc atccctccat taggtgaaga tgcttttgat gccctccctc
2521catctctccc acctccccca cctcctgcaa ggcatagtct cattgaacat tcaaaacctc
2581ctggctccag tagccggcca tcctcaggac aggatctttt tcttcttcct tcagatccct
2641ttgttgatct agcaagtggc caagttcctt tgcctcccgc tagaaggtta ccaggtgaaa
2701atgtcaaaac taacagaaca tcacaggact atgatcagct tccttcatgt tcagatggtt
2761cacaggcacc agccagaccc cctaaaccac gaccgcgcag gactgcacca gaaattcacc
2821acagaaaacc ccatgggcct gaggcggcat tggaaaatgt cgatgcaaaa attgcaaaac
2881tcatgggaga gggttatgcc tttgaagagg tgaagagagc cttagagata gcccagaata
2941atgtcgaagt tgcccggagc atcctccgag aatttgcctt ccctcctcca gtatccccac
3001gtcTAAATCT ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
3061ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG CCCGTAACTT GAAAGTATTT
3121CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTG AATGTTGTTT TAGAGTTACG
3181AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

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