Transcript: Human NR_135806.2

Homo sapiens Cbl proto-oncogene B (CBLB), transcript variant 22, non-coding RNA.

Source:
NCBI, updated 2019-07-31
Taxon:
Homo sapiens (human)
Gene:
CBLB (868)
Length:
6974
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_135806.2
NBCI Gene record:
CBLB (868)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_135806.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000381172 ACCAAACCCGGAAGCTATATT pLKO_005 1255 3UTR 100% 15.000 21.000 N CBLB n/a
2 TRCN0000315183 CTACACCTCATGACCATATAA pLKO_005 1463 3UTR 100% 15.000 21.000 N CBLB n/a
3 TRCN0000007752 CGGGATGTGTTTGGGACTAAT pLKO.1 2209 3UTR 100% 13.200 18.480 N CBLB n/a
4 TRCN0000007750 CCGGTTAAGTTGCACTCGATT pLKO.1 1278 3UTR 100% 4.950 6.930 N CBLB n/a
5 TRCN0000350496 CCGGTTAAGTTGCACTCGATT pLKO_005 1278 3UTR 100% 4.950 6.930 N CBLB n/a
6 TRCN0000007751 GCCCAGAATAATGTCGAAGTT pLKO.1 3382 3UTR 100% 4.950 6.930 N CBLB n/a
7 TRCN0000315250 GCCCAGAATAATGTCGAAGTT pLKO_005 3382 3UTR 100% 4.950 6.930 N CBLB n/a
8 TRCN0000039566 ATGCTATTCAGGATGCAGTT pLKO.1 371 3UTR 100% 0.000 0.000 N CBLB n/a
9 TRCN0000315252 ATATCAGCATTTACGACTTAT pLKO_005 534 3UTR 100% 13.200 10.560 N CBLB n/a
10 TRCN0000379559 CACCCTCCTCCCTAGCATAAA pLKO_005 2457 3UTR 100% 13.200 9.240 N CBLB n/a
11 TRCN0000007749 CCACATCAACAGCTAAATCAT pLKO.1 3901 3UTR 100% 5.625 3.938 N CBLB n/a
12 TRCN0000315181 CCACATCAACAGCTAAATCAT pLKO_005 3901 3UTR 100% 5.625 3.938 N CBLB n/a
13 TRCN0000011198 CCCTTATTTCAAGCCCTGATT pLKO.1 1366 3UTR 100% 4.950 3.465 N CBLB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_135806.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05939 pDONR223 100% 42.1% None (many diffs) n/a
2 ccsbBroad304_05939 pLX_304 0% 42.1% V5 (many diffs) n/a
3 TRCN0000465501 CTGAATGTTGTTTTAGAGTTACGA pLX_317 .6% 42.1% V5 (many diffs) n/a
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