Construct: ORF TRCN0000465525
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016296.1_s317c1
- Derived from:
- ccsbBroadEn_00914
- DNA Barcode:
- GGACTCATCTGGATCCTTTCTGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KPNA5 (3841)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465525
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | NM_001366306.2 | 100% | 100% | |
| 2 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | NM_002269.3 | 100% | 100% | |
| 3 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | NM_001366307.2 | 99.4% | 99.4% | 0_1insATGGATGCC |
| 4 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | NM_001366304.1 | 96.3% | 96.2% | 1_57del;59G>T;61_63delCGT |
| 5 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | NM_001366305.2 | 96.3% | 96.2% | 1_57del;59G>T;61_63delCGT |
| 6 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | NM_001366308.1 | 89.3% | 88.6% | (many diffs) |
| 7 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | XM_017010841.2 | 61% | 59.7% | (many diffs) |
| 8 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | XM_024446426.1 | 60.6% | 58.8% | 0_1ins547;20_21ins89 |
| 9 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | XM_024446427.1 | 60.6% | 58.8% | 0_1ins547;20_21ins89 |
| 10 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | NM_001366310.2 | 56.9% | 56.9% | 921_922ins696 |
| 11 | human | 3841 | KPNA5 | karyopherin subunit alpha 5 | NM_001366309.2 | 54.8% | 54.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1683
- ORF length:
- 1617
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgccatggct agtccaggga aagataacta tagaatgaaa agttataaga 121 ataaagccct aaatcctcaa gagatgcgta gacgaagaga agaagaagga atacagctta 181 gaaaacaaaa aagagaagaa cagttgttca aacgcagaaa tgtctatttg cccagaaatg 241 atgaatctat gcttgaaagt cctatacagg atccagatat tagttccact gtacccattc 301 cagaggaaga agttgttact acagatatgg ttcagatgat tttttctaat aatgctgatc 361 aacagctaac agcaacacag aaatttagaa agctgctttc taaagaacct aatccaccaa 421 tagatcaagt tatacagaaa ccaggagttg tacagagatt tgtgaaattt cttgaaagaa 481 atgaaaattg cactttacaa tttgaagctg catgggcatt aacaaatata gcatctggaa 541 cttttctgca taccaaggta gtgattgaaa ctggggctgt tccgattttt atcaaacttc 601 ttaattctga acatgaagat gttcaggaac aggctgtttg ggcacttggt aatattgctg 661 gtgacaatgc agaatgcaga gattttgttt tgaattgtga aatacttcca cctcttttag 721 agttattaac aaattcaaac agactcacaa caacaagaaa tgccgtgtgg gccctctcaa 781 atttatgtag aggcaaaaac cctcctccaa actttagtaa ggtttcacct tgcttaaatg 841 tcctgtcacg actgttgttt agcagtgacc cagatgtgtt agcagacgtg tgttgggccc 901 tttcttatct ctccgatgga cccaatgata aaattcaagc agtcattgat tctggagtct 961 gtcgaagatt ggtggaactt ttgatgcaca atgattataa agttgtatca cctgcattaa 1021 gggcagttgg taatattgtg actggtgatg atattcaaac acaggtaatt ttgaattgtt 1081 ctgcattacc ctgtctctta catttattga gtagcccaaa ggagtcaatt agaaaagaag 1141 cctgctggac tgtttctaac atcactgctg gaaatagagc tcagattcag gctgttatag 1201 atgcaaatat ttttcctgtt ttgattgaga ttcttcagaa agcagagttt cgtaccagaa 1261 aagaagcagc ttgggctata actaatgcaa catcaggagg tactccagag caaataaggt 1321 atttggtagc tttaggctgc attaaaCCAC TTTGTGATCT TTTGACTGTT ATGGACTCCA 1381 AAATAGTCCA AGTGGCTTTA AATGGACTTG AAAATATTTT ACGTCTTGGA GAACAAGAAT 1441 CTAAGCAGAA TGGAATAGGC ATTAATCCAT ACTGTGCTCT CATTGAAGAA GCATATGGTC 1501 TGGATAAAAT TGAGTTTTTG CAAAGCCATG AAAATCAGGA AATTTACCAG AAGGCATTTG 1561 ATCTGATTGA ACATTACTTT GGTGTAGAAG AAGATGACCC CAGCATTGTA CCTCAGGTGG 1621 ATGAAAACCA ACAACAGTTT ATATTTCAGC AGCAGGAAGC ACCAATGGAT GGATTTCAAC 1681 TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1741 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1801 GGCTTTATAT ATCTTGTGGA AAGGACGAGG ACTCATCTGG ATCCTTTCTG TAACGCGTTA 1861 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt