Transcript: Human NM_001366309.2

Homo sapiens karyopherin subunit alpha 5 (KPNA5), transcript variant 7, mRNA.

Source:
NCBI, updated 2019-07-07
Taxon:
Homo sapiens (human)
Gene:
KPNA5 (3841)
Length:
2139
CDS:
125..1108

Additional Resources:

NCBI RefSeq record:
NM_001366309.2
NBCI Gene record:
KPNA5 (3841)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001366309.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000417281 CAGTTGTTCAAACGCAGAAAT pLKO_005 320 CDS 100% 13.200 18.480 N KPNA5 n/a
2 TRCN0000064943 CCACCAATAGATCAAGTTATA pLKO.1 533 CDS 100% 13.200 9.240 N KPNA5 n/a
3 TRCN0000423556 CTATGCTTGAAAGTCCTATAC pLKO_005 366 CDS 100% 10.800 7.560 N KPNA5 n/a
4 TRCN0000064946 CCAGATATTAGTTCCACTGTA pLKO.1 392 CDS 100% 4.950 3.465 N KPNA5 n/a
5 TRCN0000064944 CCTCCAAACTTTAGTAAGGTT pLKO.1 923 CDS 100% 3.000 2.100 N KPNA5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001366309.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00914 pDONR223 100% 54.8% 54.7% None (many diffs) n/a
2 ccsbBroad304_00914 pLX_304 0% 54.8% 54.7% V5 (many diffs) n/a
3 TRCN0000465525 GGACTCATCTGGATCCTTTCTGTA pLX_317 21.1% 54.8% 54.7% V5 (many diffs) n/a
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