Construct: ORF TRCN0000465550
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007224.1_s317c1
- Derived from:
- ccsbBroadEn_13838
- DNA Barcode:
- GCCTACCGAGGCATGCGCGGTGGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CSTF3 (1479)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465550
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1479 | CSTF3 | cleavage stimulation factor... | NM_001033505.1 | 99.6% | 2.9% | 12delC |
| 2 | human | 1479 | CSTF3 | cleavage stimulation factor... | NM_001033506.1 | 41.7% | 6.8% | 12delC;130_131delGTinsAA;132_133ins177 |
| 3 | human | 1479 | CSTF3 | cleavage stimulation factor... | NM_001326.3 | 13.2% | .9% | (many diffs) |
| 4 | mouse | 228410 | Cstf3 | cleavage stimulation factor... | NM_177253.4 | 89.4% | 2.8% | (many diffs) |
| 5 | mouse | 228410 | Cstf3 | cleavage stimulation factor... | NM_001037326.2 | 40.1% | 6.8% | (many diffs) |
| 6 | mouse | 228410 | Cstf3 | cleavage stimulation factor... | NM_145529.3 | 12.9% | .9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 117
- ORF length:
- 51
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgGCATGTC AGGAGAGGAG CCACGGAGCA GGCAGCTGAG TATGTCCCAG AGAAGGTGAA 121 GAAAGCGGAA AAGAAATTAG AAGAGAATCC ATATGACCTT GATGCTTGGA GCATTCTCAT 181 TCGAGAGGCA CAGAATCAAC CTATAGACAA AGCACGGAAG ACTTATGAAC GCCTTGTTGC 241 CCAGTTCCCC AGTTCTGGCA GATTCTGGAA ACTGTACATT GAAGCAGAGG TTACTATTTT 301 ATTTTATTTT TTCTTATATC AGTATTGCAG CATTCACTGT AGTGATAGAA AACAAGTTAG 361 GAACATAGCC AATTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 421 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 481 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CCTACCGAGG CATGCGCGGT 541 GGCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att