Construct: ORF TRCN0000465554
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011212.1_s317c1
- Derived from:
- BRDN0000395123
- DNA Barcode:
- ACCGCACTCGCACGGCGTGGGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRMPD4 (9758)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465554
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | NM_014728.3 | 99.8% | 99.8% | 23_24delAGinsCT;3566_3567delGCinsAG |
| 2 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | NM_001368401.1 | 99.2% | 98.9% | (many diffs) |
| 3 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | NM_001368402.2 | 99.2% | 98.9% | (many diffs) |
| 4 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | NM_001368399.2 | 99.1% | 98.9% | (many diffs) |
| 5 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | XM_017029985.1 | 97.4% | 97.2% | (many diffs) |
| 6 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | NM_001368400.2 | 96.9% | 96.8% | 0_1ins120;3446_3447delGCinsAG |
| 7 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | NM_001368398.2 | 96.3% | 96.4% | (many diffs) |
| 8 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | NM_001368397.1 | 74.9% | 74.8% | (many diffs) |
| 9 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | NM_001368396.2 | 74.8% | 74.7% | (many diffs) |
| 10 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | XM_017029983.1 | 74.4% | 74.1% | (many diffs) |
| 11 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | XM_005274632.3 | 73.5% | 73.2% | (many diffs) |
| 12 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | NM_001368395.2 | 72.8% | 72.8% | (many diffs) |
| 13 | human | 9758 | FRMPD4 | FERM and PDZ domain contain... | XM_017029984.1 | 71.8% | 71.5% | (many diffs) |
| 14 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | XM_017318529.1 | 87.4% | 89.7% | (many diffs) |
| 15 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | NM_001033330.3 | 87.2% | 89.7% | (many diffs) |
| 16 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | NM_001290427.1 | 87.2% | 89.7% | (many diffs) |
| 17 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | XM_017318528.1 | 86.4% | 88.7% | (many diffs) |
| 18 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | NM_001290428.1 | 85.1% | 87.7% | (many diffs) |
| 19 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | XM_006528887.3 | 65.9% | 67.8% | (many diffs) |
| 20 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | XM_006528888.3 | 65.6% | 67.2% | (many diffs) |
| 21 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | XM_011247832.2 | 65.4% | 67.2% | (many diffs) |
| 22 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | XM_011247833.2 | 65.4% | 67.2% | (many diffs) |
| 23 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | XM_011247834.2 | 65.4% | 67.2% | (many diffs) |
| 24 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | XM_006528884.3 | 65.1% | 66.7% | (many diffs) |
| 25 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | XM_006528885.3 | 65.1% | 66.7% | (many diffs) |
| 26 | mouse | 333605 | Frmpd4 | FERM and PDZ domain contain... | XM_006528889.2 | 63.8% | 65.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4035
- ORF length:
- 3966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgtcttc agctttgtga ctattgcaaa gctttcgagc cacaggacga 121 agtcttcagg ctggccgcct ccctcgggaa cctggggctt gagccaggtg ccgccctatg 181 gatgggagat gacggcaaac cgagatgggc gagactactt catcaatcac atgacacagg 241 caatcccttt tgacgaccct cggttagaga gctgccaaat catccctccg gctcctcgga 301 aggtggagat gagaagggac cccgtgctgg gatttggttt tgtggcaggc agtgaaaagc 361 cagtggtcgt tcgctcagta acaccaggtg gcccctctga aggcaagctg atcccgggag 421 atcagattgt aatgattaat gatgaaccgg tcagcgctgc acccagagag cgggtcatcg 481 atctggtcag aagctgcaaa gaatcgatac tcctcactgt cattcagcct tacccttctc 541 ccaaatcagc atttattagt gctgcaaaaa aggcaagatt aaagtccaat cctgtcaaag 601 tacgcttctc tgaggaggtc atcatcaacg gccaagtgtc ggaaactgtt aaggacaact 661 cacttctttt tatgccaaat gttttgaaag tctatctgga aaatgggcag accaaatcat 721 ttcgttttga ctgcagcact tccattaagg atgtcatctt aacccttcaa gagaagctct 781 ccatcaaagg cattgaacac ttctctctca tgctggagca gaggacagaa ggggctggaa 841 cgaagctgct cttgcttcat gaacaggaga ctctaactca ggtgacacag aggcccagct 901 cccataagat gagatgtctt ttccgaatta gcttcgtccc aaaagatcca attgaccttt 961 taaggagaga tccagttgct ttcgagtatc tctatgttca gagttgtaac gatgtggttc 1021 aggagcgatt tgggccggag ctgaaatatg acatagccct gcggctggcc gcattacaaa 1081 tgtacattgc aaccgttacc accaagcaaa cgcagaaaat ctccctcaaa tacatcgaaa 1141 aagaatgggg attagagact tttcttccct ctgctgtgct gcaaagcatg aaagagaaga 1201 acataaagaa agcactttca caccttgtca aagcaaatca aaacttggta ccaccgggta 1261 aaaagctctc tgcactacaa gccaaggtcc attatctcaa gttcctcagt gacctacgat 1321 tgtatggggg ccgtgtgttc aaggcaacat tagtgcaggc agaaaagcgc tcggaagtga 1381 ctctcctggt tgggccccgg tatggcataa gccatgtcat caacaccaaa accaatctgg 1441 tggctctttt agccgacttt agccacgtca acaggatcga aatgttttcc gaggaggaga 1501 gcttggtgcg ggtagaactc cacgtgctag atgtgaagcc tatcacgctt ctgatggaat 1561 cctcagatgc catgaacctg gcctgcttga cggctggata ctaccggctg cttgttgatt 1621 ccaggaggtc gatatttaac atggccaaca agaaaaacac agcgacccag gaaacaggac 1681 ctgaaaacaa ggggaagcat aacctccttg gcccagattg gaactgtata ccccaaatga 1741 ccacctttat tggcgaaggg gaacaagaag cccagataac atacatagat tcaaagcaga 1801 agacggtgga gatcacagac agcaccatgt gtccaaaaga gcaccggcac ttgtacatag 1861 acaatgccta tagttcagat ggacttaacc agcagctgag ccagcccggg gaggccccct 1921 gtgaggcaga ctacagaagt ctagctcagc ggtccctatt gaccctctca ggaccagaaa 1981 ctctgaagaa agcacaggaa tctccgagag gagctaaagt gtcctttatt tttggagact 2041 tcgccttgga tgatggtatt agtcccccaa cccttggcta tgaaacgcta ctagatgagg 2101 gtcctgaaat gctggagaag cagagaaatc tctacattgg cagtgccaat gacatgaagg 2161 gcctggatct cactccagag gcagagggca tccagtttgt ggaaaattct gtttatgcaa 2221 acataggcga tgtgaagagc ttccaggccg cggaggggat cgaggaaccc ctcttgcatg 2281 acatctgtta tgcagaaaac actgatgacg cggaggacga ggacgaggtg agctgcgagg 2341 aggacctcgt ggtgggggag atgaaccagc cggccatcct caacctgtct gggtcaagcg 2401 atgacatcat tgacctcaca tccctgcccc ctccagaagg tgatgacaat gaggatgact 2461 tcctgttgcg ttccttgaac atggccattg ccgcaccccc acctggcttt agagacagtt 2521 cagatgaaga ggactctcag agccaggcag cttccttccc cgaggacaag gagaaaggca 2581 gcagcctgca aaatgatgag atccccgtgt ccctcattga cgctgtgccc accagcgccg 2641 aaggcaagtg tgagaaggga ctggataatg ccgtcgtctc cacgctggga gctctagagg 2701 ctctatccgt gtcagaagaa cagcagacca gtgacaattc aggtgtagcc atcttgcggg 2761 cttatagtcc tgagtcttcg tcagactcgg gcaatgaaac taactcttct gaaatgactg 2821 agagttctga actggccaca gcacaaaaac agtcagaaaa cctctcccgc atgttcttgg 2881 ccactcacga aggctaccac ccccttgcag aagagcagac cgagttcccg gcctccaaga 2941 cccccgctgg gggcttgcct ccaaagtcct cgcacgccct ggctgctagg ccagcaaccg 3001 acctcccgcc caaagttgtg ccttccaagc agttacttca ctcagaccac atggagatgg 3061 agcctgaaac tatggagact aagtcggtca ctgactattt tagcaaactg cacatggggt 3121 cggtggcata ctcctgcact agcaaaagga aaagcaagct ggccgatggt gaggggaagg 3181 caccccctaa tgggaacaca acaggaaaaa aacagcaggg gaccaaaacg gcagagatgg 3241 aggaggaggc cagtggtaaa tttggtactg tgtcttcacg agacagtcaa cacctgagca 3301 cttttaatct ggagagaact gcctttcgca aggacagtca aagatggtat gtggccactg 3361 aaggtgggat ggctgaaaaa agtggattag aagcagcaac agggaaaacc tttccaagag 3421 cttctggtct tggggcaagg gaggccgaag ggaaggaaga aggagctcct gatggagaaa 3481 ccagtgatgg ctcaggactt ggtcaagggg accgcttctt aactgacgtg acctgtgcat 3541 cttcagccaa agacttagat aacccagagg acgctgactc gtccacctgc gaccatcctt 3601 ccaagcttcc tgaggctgat gagagtgtgg cccagctttg tgactaccac ttggccaagc 3661 ggatgtcatc actgcaaagc gagggccatt tttctctgca gagctcccaa ggctcttcag 3721 tggatgcagg ctgtggcaca ggcagcagtg gcagtgcctg tgcCACACCC GTGGAGTCGC 3781 CGCTCTGCCC CTCCCTGGGG AAGCACTTGA TTCCTGACGC TTCTGGGAAA GGCGTGAATT 3841 ACATTCCTTC AGAGGAGAGA GCCCCTGGGC TTCCCAACCA CGGAGCCACC TTTAAGGAAC 3901 TGCACCCACA GACAGAAGGG ATGTGTCCAC GGATGACAGT GCCTGCTCTG CACACAGCCA 3961 TTAACACCGA ACCCCTGTTT GGCACATTGA GAGATGGATG CCATCGGCTC CCCAAGATTA 4021 AGGAAACCAC AGTGTTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 4081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 4141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ACCGCACTCG CACGGCGTGG 4201 GCACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt