Construct: ORF TRCN0000465576
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015288.1_s317c1
- Derived from:
- ccsbBroadEn_12592
- DNA Barcode:
- TTCAATATGGCCTGAGTCCGTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VTCN1 (79679)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465576
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79679 | VTCN1 | V-set domain containing T c... | NM_001253849.1 | 100% | 100% | |
| 2 | human | 79679 | VTCN1 | V-set domain containing T c... | NM_001253850.1 | 82.3% | 71.8% | (many diffs) |
| 3 | human | 79679 | VTCN1 | V-set domain containing T c... | NM_024626.4 | 66.3% | 66.3% | 1_285del |
| 4 | human | 79679 | VTCN1 | V-set domain containing T c... | XM_017002335.2 | 65.1% | 65.1% | 1_300del |
| 5 | human | 79679 | VTCN1 | V-set domain containing T c... | XM_011542143.2 | 56.3% | 56.3% | 1_435del |
| 6 | human | 79679 | VTCN1 | V-set domain containing T c... | NR_045603.2 | 20.8% | 1_447del;1009_2695del | |
| 7 | human | 79679 | VTCN1 | V-set domain containing T c... | NR_045604.2 | 13.6% | 1_357del;431_432ins206;713_2399del | |
| 8 | mouse | 242122 | Vtcn1 | V-set domain containing T c... | NM_178594.3 | 56.3% | 59% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 627
- ORF length:
- 561
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cagaggccgg acagcagtgt ttgctgatca agtgatagtt ggcaatgcct 121 ctttgcggct gaaaaacgtg caactcacag atgctggcac ctacaaatgt tatatcatca 181 cttctaaagg caaggggaat gctaaccttg agtataaaac tggagccttc agcatgccgg 241 aagtgaatgt ggactataat gccagctcag agacctTGCG GTGTGAGGCT CCCCGATGGT 301 TCCCCCAGCC CACAGTGGTC TGGGCATCCC AAGTTGACCA GGGAGCCAAC TTCTCGGAAG 361 TCTCCAATAC CAGCTTTGAG CTGAACTCTG AGAATGTGAC CATGAAGGTT GTGTCTGTGC 421 TCTACAATGT TACGATCAAC AACACATACT CCTGTATGAT TGAAAATGAC ATTGCCAAAG 481 CAACAGGGGA TATCAAAGTG ACAGAATCGG AGATCAAAAG GCGGAGTCAC CTACAGCTGC 541 TAAACTCAAA GGCTTCTCTG TGTGTCTCTT CTTTCTTTGC CATCAGCTGG GCACTTCTGC 601 CTCTCAGCCC TTACCTGATG CTAAAATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 661 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 721 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATTCAATAT 781 GGCCTGAGTC CGTGCGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 841 aagatt