Construct: ORF TRCN0000465581
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011803.1_s317c1
- Derived from:
- ccsbBroadEn_06362
- DNA Barcode:
- GAGGAACTTTTGAAGCTGCAGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HGF (3082)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465581
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3082 | HGF | hepatocyte growth factor | NM_001010934.3 | 99.8% | 100% | 129A>G |
| 2 | human | 3082 | HGF | hepatocyte growth factor | NM_001010931.3 | 72% | 71.7% | (many diffs) |
| 3 | human | 3082 | HGF | hepatocyte growth factor | XM_017012097.1 | 72% | 71.7% | (many diffs) |
| 4 | human | 3082 | HGF | hepatocyte growth factor | NM_001010933.3 | 70.3% | 70% | (many diffs) |
| 5 | human | 3082 | HGF | hepatocyte growth factor | XM_017012098.1 | 70.3% | 70% | (many diffs) |
| 6 | human | 3082 | HGF | hepatocyte growth factor | NM_000601.6 | 28.7% | 28.5% | (many diffs) |
| 7 | human | 3082 | HGF | hepatocyte growth factor | XM_006715956.2 | 28.7% | 28.5% | (many diffs) |
| 8 | human | 3082 | HGF | hepatocyte growth factor | NM_001010932.3 | 28% | 27.8% | (many diffs) |
| 9 | human | 3082 | HGF | hepatocyte growth factor | XM_011516115.2 | 28% | 27.8% | (many diffs) |
| 10 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289460.2 | 88.1% | 89% | (many diffs) |
| 11 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289458.1 | 25.4% | 25.5% | (many diffs) |
| 12 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289459.1 | 25.4% | 25.5% | (many diffs) |
| 13 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_010427.5 | 25.4% | 25.5% | (many diffs) |
| 14 | mouse | 15234 | Hgf | hepatocyte growth factor | NM_001289461.1 | 24.7% | 24.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 696
- ORF length:
- 630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg ggtgaccaaa ctcctgccag ccctgctgct gcagcatgtc ctcctgcatc 121 tcctcctgct ccccatcgcc atcccctatg cagagggaca aaggaaaaga agaaatacaa 181 ttcatgaatt caagaaatca gcaaagacta ccctaatcaa aatagatcca gcactgaaga 241 taaaaaccaa aaaagtgaat actgcagacc aatgtgctaa tagatgtact aggaataaag 301 gacttccatt cacttgcaag gcttttgttt ttgataaagc aagaaaacaa tgcctctggt 361 tccccttcaa tagcatgtca agtggagtga aaaaagaatt tggccatgaa tttgacctct 421 atgaaaacaa agactacatt agaaactgca tcattggtaa aggacgcagc tacaagggaa 481 cagtatCTAT CACTAAGAGT GGCATCAAAT GTCAGCCCTG GAGTTCCATG ATACCACACG 541 AACACAGCTT TTTGCCTTCG AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA 601 ATCCTCGAGG GGAAGAAGGG GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG 661 AAGTCTGTGA CATTCCTCAG TGTTCAGAAG GTAAATACCC AACTTTCTTG TACAAAGTGG 721 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 781 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 841 AGAGGAACTT TTGAAGCTGC AGCAAACGCG TTAAGTCgac aatcaacctc tggattacaa 901 aatttgtgaa agatt