Construct: ORF TRCN0000465581

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011803.1_s317c1
Derived from:
ccsbBroadEn_06362
DNA Barcode:
GAGGAACTTTTGAAGCTGCAGCAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HGF (3082)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465581
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3082 HGF hepatocyte growth factor NM_001010934.3 99.8% 100% 129A>G
2 human 3082 HGF hepatocyte growth factor NM_001010931.3 72% 71.7% (many diffs)
3 human 3082 HGF hepatocyte growth factor XM_017012097.1 72% 71.7% (many diffs)
4 human 3082 HGF hepatocyte growth factor NM_001010933.3 70.3% 70% (many diffs)
5 human 3082 HGF hepatocyte growth factor XM_017012098.1 70.3% 70% (many diffs)
6 human 3082 HGF hepatocyte growth factor NM_000601.6 28.7% 28.5% (many diffs)
7 human 3082 HGF hepatocyte growth factor XM_006715956.2 28.7% 28.5% (many diffs)
8 human 3082 HGF hepatocyte growth factor NM_001010932.3 28% 27.8% (many diffs)
9 human 3082 HGF hepatocyte growth factor XM_011516115.2 28% 27.8% (many diffs)
10 mouse 15234 Hgf hepatocyte growth factor NM_001289460.2 88.1% 89% (many diffs)
11 mouse 15234 Hgf hepatocyte growth factor NM_001289458.1 25.4% 25.5% (many diffs)
12 mouse 15234 Hgf hepatocyte growth factor NM_001289459.1 25.4% 25.5% (many diffs)
13 mouse 15234 Hgf hepatocyte growth factor NM_010427.5 25.4% 25.5% (many diffs)
14 mouse 15234 Hgf hepatocyte growth factor NM_001289461.1 24.7% 24.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
696
ORF length:
630
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg ggtgaccaaa ctcctgccag ccctgctgct gcagcatgtc ctcctgcatc
121tcctcctgct ccccatcgcc atcccctatg cagagggaca aaggaaaaga agaaatacaa
181ttcatgaatt caagaaatca gcaaagacta ccctaatcaa aatagatcca gcactgaaga
241taaaaaccaa aaaagtgaat actgcagacc aatgtgctaa tagatgtact aggaataaag
301gacttccatt cacttgcaag gcttttgttt ttgataaagc aagaaaacaa tgcctctggt
361tccccttcaa tagcatgtca agtggagtga aaaaagaatt tggccatgaa tttgacctct
421atgaaaacaa agactacatt agaaactgca tcattggtaa aggacgcagc tacaagggaa
481cagtatCTAT CACTAAGAGT GGCATCAAAT GTCAGCCCTG GAGTTCCATG ATACCACACG
541AACACAGCTT TTTGCCTTCG AGCTATCGGG GTAAAGACCT ACAGGAAAAC TACTGTCGAA
601ATCCTCGAGG GGAAGAAGGG GGACCCTGGT GTTTCACAAG CAATCCAGAG GTACGCTACG
661AAGTCTGTGA CATTCCTCAG TGTTCAGAAG GTAAATACCC AACTTTCTTG TACAAAGTGG
721TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
781CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
841AGAGGAACTT TTGAAGCTGC AGCAAACGCG TTAAGTCgac aatcaacctc tggattacaa
901aatttgtgaa agatt

Download FASTA (ORF) (Full)